SRR1544964_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544964_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1837075
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	5365	0.29204033585999484	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	4712	0.25649469945429554	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	4399	0.23945674509750553	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4105	0.22345304356109577	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	3816	0.2077215138195229	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3005	0.16357524869697754	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	2729	0.1485513656219806	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2346	0.12770300613747396	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2184	0.11888463998475837	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1911	0.10402405998666357	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	20	6.312446E-4	46.0	15
ACGCAAC	20	6.312446E-4	46.0	31
CTAACGG	120	0.0	46.0	1
TCGTATC	20	6.312446E-4	46.0	45
GTATCGG	20	6.312446E-4	46.0	1
TACGGGA	340	0.0	43.294117	3
ACGTAGG	160	0.0	43.125	1
CTTACGT	80	0.0	43.125	22
GCTACGA	340	0.0	42.617645	10
GGACTAA	585	0.0	42.46154	7
TTACGGG	570	0.0	42.368423	2
CGCACTT	1195	0.0	41.76569	34
CGTAGGG	540	0.0	41.74074	2
TAGTAGG	555	0.0	41.44144	1
ATCAACG	1425	0.0	41.157894	14
ACGGGAT	590	0.0	40.9322	4
CGCAAGC	1425	0.0	40.835087	19
GCGATTG	175	0.0	40.74286	8
AATGCGG	215	0.0	40.65116	1
TATGACC	1360	0.0	40.588234	27