##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544964_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1837075
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94498754814039	34.0	33.0	34.0	31.0	34.0
2	33.26786331532463	34.0	33.0	34.0	31.0	34.0
3	33.33292652722398	34.0	34.0	34.0	31.0	34.0
4	36.59868159982581	37.0	37.0	37.0	35.0	37.0
5	36.600373964046106	37.0	37.0	37.0	35.0	37.0
6	36.66562170842236	37.0	37.0	37.0	35.0	37.0
7	36.46775934569901	37.0	37.0	37.0	35.0	37.0
8	36.50201053304846	37.0	37.0	37.0	35.0	37.0
9	38.32242668372277	39.0	39.0	39.0	37.0	39.0
10	38.18329137351496	39.0	39.0	39.0	35.0	39.0
11	37.7381919627669	39.0	37.0	39.0	35.0	39.0
12	37.68916565736293	39.0	37.0	39.0	35.0	39.0
13	37.68929521113727	39.0	37.0	39.0	35.0	39.0
14	39.07625382741586	41.0	38.0	41.0	35.0	41.0
15	39.11642420695943	41.0	38.0	41.0	35.0	41.0
16	39.111575738606206	41.0	38.0	41.0	35.0	41.0
17	39.14022617476151	41.0	38.0	41.0	35.0	41.0
18	39.11768871711825	41.0	38.0	41.0	35.0	41.0
19	39.070994923996025	41.0	37.0	41.0	35.0	41.0
20	39.00045724861533	41.0	37.0	41.0	35.0	41.0
21	38.944691969571196	41.0	37.0	41.0	35.0	41.0
22	38.91528544016983	41.0	37.0	41.0	35.0	41.0
23	38.8956509668903	41.0	37.0	41.0	35.0	41.0
24	38.8228994461304	41.0	36.0	41.0	35.0	41.0
25	38.78014452322306	41.0	36.0	41.0	35.0	41.0
26	38.72460079201992	41.0	36.0	41.0	35.0	41.0
27	38.688086768368194	41.0	36.0	41.0	35.0	41.0
28	38.703139501653446	41.0	36.0	41.0	35.0	41.0
29	38.720371242328156	41.0	36.0	41.0	35.0	41.0
30	38.65805696555666	41.0	36.0	41.0	35.0	41.0
31	38.60447069390199	41.0	36.0	41.0	35.0	41.0
32	38.52933277084496	41.0	36.0	41.0	35.0	41.0
33	38.43999618959487	40.0	36.0	41.0	35.0	41.0
34	38.3589091354463	40.0	35.0	41.0	35.0	41.0
35	38.28283276404066	40.0	35.0	41.0	35.0	41.0
36	38.223158553679085	40.0	35.0	41.0	35.0	41.0
37	38.15493814895962	40.0	35.0	41.0	35.0	41.0
38	38.06332893322265	40.0	35.0	41.0	35.0	41.0
39	37.954848332267325	40.0	35.0	41.0	35.0	41.0
40	37.85387150769566	40.0	35.0	41.0	35.0	41.0
41	37.81734605282855	40.0	35.0	41.0	35.0	41.0
42	37.74672019378632	40.0	35.0	41.0	35.0	41.0
43	37.65685124450553	40.0	35.0	41.0	35.0	41.0
44	37.58204700406897	40.0	35.0	41.0	35.0	41.0
45	37.526010641917175	39.0	35.0	41.0	34.0	41.0
46	37.526198440455616	39.0	35.0	41.0	35.0	41.0
47	37.461459112992124	39.0	35.0	41.0	35.0	41.0
48	37.43366220758543	39.0	35.0	41.0	34.0	41.0
49	37.36999795871154	39.0	35.0	41.0	34.0	41.0
50	37.296825116013224	39.0	35.0	41.0	34.0	41.0
51	37.218580624089924	39.0	35.0	41.0	34.0	41.0
52	37.01360205761877	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	7.0
13	18.0
14	18.0
15	31.0
16	52.0
17	78.0
18	165.0
19	259.0
20	433.0
21	693.0
22	1083.0
23	1735.0
24	3023.0
25	4877.0
26	7029.0
27	8506.0
28	9042.0
29	9733.0
30	11478.0
31	14196.0
32	18175.0
33	25974.0
34	79455.0
35	273908.0
36	74629.0
37	118015.0
38	202275.0
39	962334.0
40	9851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.86396853694052	21.949457697698787	22.755521685287754	19.43105208007294
2	28.74183144400746	24.094824653321176	24.939047126546278	22.22429677612509
3	29.19369105779568	23.985792632309515	24.12503572254807	22.695480587346733
4	26.40235156430739	26.830804403739638	20.939319298341115	25.827524733611856
5	25.06223208088946	36.65217805478818	19.656192588762025	18.629397275560333
6	86.771797558619	3.848454744634813	6.76766054733748	2.6120871494087066
7	85.89409795462896	4.000489909230706	5.610440510049944	4.494971626090388
8	73.93323625872651	7.375202427772409	11.169168379080876	7.522392934420206
9	46.528367105316875	22.843052134507303	15.381788985207463	15.24679177496836
10	35.49359715852646	23.01511914320319	25.223956561381545	16.267327136888802
11	35.92210443231768	17.72186764285617	26.276989235605512	20.079038689220635
12	22.69945429555135	27.07820856524638	28.753153790672677	21.46918334852959
13	17.07796361063103	29.06713117319652	30.198277152538683	23.656628063633768
14	16.202278077922784	25.045575167045442	38.126913707932445	20.62523304709933
15	18.988228569873304	19.944041478981532	39.548358123647645	21.519371827497515
16	19.926132574881265	22.5987507314617	27.929398636419307	29.54571805723773
17	20.641889960943345	24.269395642529563	29.754963733108337	25.33375066341875
18	23.646666575942735	27.904195528217414	26.78192234938693	21.66721554645292
19	27.229209477021897	23.450920621096035	25.84276635412272	23.477103547759345
20	24.256222527659457	28.138589877930947	25.879727283861577	21.725460310548016
21	21.77608426438768	24.57286719377271	25.431188165970358	28.21986037586925
22	22.19501109100064	26.68312398786114	25.01449314807507	26.10737177306316
23	22.08358395819441	22.316290842779964	32.46350856660724	23.136616632418384
24	22.093055536654738	22.294625967910946	29.82387763156104	25.788440863873273
25	19.344120408802034	27.191540900616467	28.648585387096336	24.81575330348516
26	19.893090918988065	26.64850373555788	28.406298055332524	25.052107290121523
27	21.327327409060597	25.86170951104337	32.08018181075895	20.73078126913708
28	20.31833213124124	25.429990610073077	28.336077732264602	25.91559952642108
29	21.116503136779937	25.245349264455726	28.779336717335983	24.858810881428358
30	24.556482451723525	23.80387300464053	25.673094457221396	25.966550086414546
31	26.59771647864132	23.789230162078304	26.19876706176939	23.41428629751099
32	23.282446280091996	25.549963937237187	27.111195786780616	24.056393995890204
33	19.059210973966767	24.369772600465414	30.245743913558236	26.32527251200958
34	21.958493801287375	23.444334063660982	29.77543105208007	24.82174108297157
35	23.824775798484	26.091531374603647	25.775975395669747	24.3077174312426
36	22.798143788359212	28.243593756379028	25.28492304342501	23.673339411836753
37	23.031830491406176	26.711429854524177	26.921002136548587	23.33573751752106
38	22.22636528176585	27.95487391641604	24.367812963542587	25.450947838275518
39	25.31420872854946	25.060163575248694	27.497461998013144	22.128165698188695
40	18.093708748962346	23.985411591796744	31.42403004776615	26.496849611474765
41	20.8315392675857	26.122776696650924	26.492440428398407	26.55324360736497
42	21.71773063157465	22.679857926323095	29.02309377679191	26.57931766531034
43	22.4385504130207	25.897472884884937	28.29106051739858	23.37291618469578
44	20.194766136385287	26.285535429963392	28.14887797177579	25.37082046187554
45	21.604833771076304	25.68839051209123	26.63364315555979	26.073132561272676
46	22.917463903215708	23.20063143856402	29.01917450294626	24.86273015527401
47	21.876352353605597	21.883592123348258	31.507967829293847	24.732087693752298
48	21.155532572159547	22.047548412558008	28.204401017922514	28.592517997359934
49	20.45708531224909	23.7660411251582	30.912401507831746	24.864472054760963
50	20.259760760992336	24.05982336050515	28.859409659377	26.821006219125508
51	20.22998516663718	22.173074044336786	30.18483186587374	27.412108923152296
52	21.482737503912468	20.61418287222895	32.48109086455371	25.421988759304874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	679.0
2	1154.0
3	4560.5
4	7967.0
5	5301.5
6	2636.0
7	2582.5
8	2529.0
9	2537.5
10	2546.0
11	2511.5
12	2477.0
13	2290.5
14	2088.5
15	2073.0
16	1986.0
17	1899.0
18	2236.0
19	2573.0
20	2890.5
21	3208.0
22	3808.5
23	4409.0
24	5627.0
25	6845.0
26	8360.0
27	9875.0
28	14723.0
29	19571.0
30	20019.5
31	20468.0
32	22127.0
33	23786.0
34	28376.0
35	32966.0
36	34143.5
37	35321.0
38	41170.5
39	55141.5
40	63263.0
41	77641.0
42	92019.0
43	117166.0
44	142313.0
45	163211.5
46	184110.0
47	187679.5
48	191249.0
49	175558.0
50	159867.0
51	139225.5
52	118584.0
53	110007.5
54	101431.0
55	95607.0
56	89783.0
57	85484.5
58	81186.0
59	81771.0
60	82356.0
61	82517.5
62	82679.0
63	76012.0
64	59566.5
65	49788.0
66	41186.0
67	32584.0
68	27135.5
69	21687.0
70	18923.5
71	16160.0
72	13688.5
73	11217.0
74	9266.0
75	7315.0
76	6026.5
77	4738.0
78	3337.0
79	1936.0
80	1536.5
81	1137.0
82	769.5
83	402.0
84	306.0
85	210.0
86	139.0
87	68.0
88	38.5
89	6.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1837075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.9901953263873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83656443853901	14.658831284887672
2	11.967343406317434	5.023937512731122
3	4.478915627085805	2.8203994158881853
4	2.227521704248818	1.8702446266379922
5	1.2738102413285863	1.3368762887119787
6	0.7961700284178431	1.0027058645703513
7	0.6204360634876759	0.9116151912098802
8	0.44055323680581265	0.7397838793780933
9	0.36778171981592006	0.6947829122769729
>10	5.857448822219322	32.647770525807076
>50	1.8923736521245447	25.759697932826036
>100	0.2231171364118724	7.72504937695616
>500	0.010674215233184223	1.6041710096515334
>1k	0.007029361251121318	2.9109515020309273
>5k	2.6034671300449323E-4	0.29318267643605783
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	5365	0.29204033585999484	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	4712	0.25649469945429554	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	4399	0.23945674509750553	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4105	0.22345304356109577	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	3816	0.2077215138195229	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3005	0.16357524869697754	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	2729	0.1485513656219806	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2346	0.12770300613747396	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2184	0.11888463998475837	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1911	0.10402405998666357	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2434848876610917	0.0
2	0.0	0.0	0.0	0.9474300178272526	0.0
3	0.0	0.0	0.0	1.5124586639086592	0.0
4	0.0	0.0	0.0	1.89039641821918	0.0
5	5.443435896738021E-5	0.0	0.0	3.9456745097505546	0.0
6	5.443435896738021E-5	0.0	0.0	4.358776859954003	0.0
7	5.443435896738021E-5	0.0	0.0	6.116571179728645	0.0
8	5.443435896738021E-5	0.0	0.0	8.078712083066831	0.0
9	5.443435896738021E-5	0.0	0.0	9.843201828994461	0.0
10	5.443435896738021E-5	0.0	0.0	10.740552236571723	0.0
11	5.443435896738021E-5	0.0	0.0	11.605133160050624	0.0
12	5.443435896738021E-5	0.0	0.0	12.33743859123879	0.0
13	5.443435896738021E-5	0.0	0.0	12.62980553325259	0.0
14	5.443435896738021E-5	0.0	0.0	12.848468353224556	0.0
15	5.443435896738021E-5	0.0	0.0	13.56749180082468	0.0
16	5.443435896738021E-5	0.0	0.0	14.331859069444633	0.0
17	5.443435896738021E-5	0.0	0.0	15.290067090347428	0.0
18	5.443435896738021E-5	0.0	0.0	15.787488262591348	0.0
19	1.0886871793476042E-4	0.0	0.0	16.422138453792034	0.0
20	1.0886871793476042E-4	0.0	0.0	17.117972864472055	0.0
21	1.0886871793476042E-4	0.0	0.0	17.76051603772301	0.0
22	1.0886871793476042E-4	0.0	0.0	18.446116788917166	0.0
23	1.0886871793476042E-4	0.0	0.0	18.952410761672766	0.0
24	1.6330307690214064E-4	0.0	0.0	19.404596981614795	0.0
25	2.1773743586952083E-4	0.0	0.0	19.82896724412449	0.0
26	2.1773743586952083E-4	0.0	0.0	20.24119864458446	0.0
27	2.1773743586952083E-4	0.0	0.0	20.648857558891173	0.0
28	2.1773743586952083E-4	0.0	0.0	21.053304846018808	0.0
29	2.1773743586952083E-4	0.0	0.0	21.55339330185213	0.0
30	2.1773743586952083E-4	0.0	0.0	22.042486017174042	0.0
31	2.1773743586952083E-4	0.0	0.0	22.50664779608889	0.0
32	2.1773743586952083E-4	0.0	0.0	22.94430004218663	0.0
33	2.1773743586952083E-4	0.0	0.0	23.33807819495666	0.0
34	2.1773743586952083E-4	0.0	0.0	23.833920770790524	0.0
35	2.1773743586952083E-4	0.0	0.0	24.25535157791598	0.0
36	2.1773743586952083E-4	0.0	0.0	24.700951240422956	0.0
37	2.1773743586952083E-4	0.0	0.0	25.1467686403658	0.0
38	2.1773743586952083E-4	0.0	0.0	25.57832423825919	0.0
39	2.7217179483690103E-4	0.0	0.0	26.044500088455834	0.0
40	2.7217179483690103E-4	0.0	0.0	26.53762094634133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	20	6.312446E-4	46.0	15
ACGCAAC	20	6.312446E-4	46.0	31
CTAACGG	120	0.0	46.0	1
TCGTATC	20	6.312446E-4	46.0	45
GTATCGG	20	6.312446E-4	46.0	1
TACGGGA	340	0.0	43.294117	3
ACGTAGG	160	0.0	43.125	1
CTTACGT	80	0.0	43.125	22
GCTACGA	340	0.0	42.617645	10
GGACTAA	585	0.0	42.46154	7
TTACGGG	570	0.0	42.368423	2
CGCACTT	1195	0.0	41.76569	34
CGTAGGG	540	0.0	41.74074	2
TAGTAGG	555	0.0	41.44144	1
ATCAACG	1425	0.0	41.157894	14
ACGGGAT	590	0.0	40.9322	4
CGCAAGC	1425	0.0	40.835087	19
GCGATTG	175	0.0	40.74286	8
AATGCGG	215	0.0	40.65116	1
TATGACC	1360	0.0	40.588234	27
>>END_MODULE