FastQCFastQC Report
Thu 26 May 2016
SRR1544963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544963_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1122337
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT44690.39818699731007706No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA44580.39720689953195876No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT35940.3202246740506639Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT31760.2829809584821671No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.22274949502689478No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTTG22590.2012764437063021Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18170.16189433298554712No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT16510.14710376651576132No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT13870.12358141984092122No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13850.12340322024489969No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG206.311389E-446.00000436
AAACTCG206.311389E-446.00000428
CGTATGA206.311389E-446.00000438
TCGGTAC206.311389E-446.00000435
CGGTCTA301.8613646E-646.030
ACGTAGG650.046.01
ACATCGG253.417104E-546.01
CGATACG301.8613646E-646.09
AATGCGG1550.043.0322571
CTAACGG1300.042.461541
TCGGCGT6950.042.3597154
GCGATTG1200.042.1666688
CGCACTT7200.042.16666834
CATGCGG2700.041.740741
CGAATAT1600.041.68750414
CCGCACT7300.041.58904333
CGTTTTT18250.041.589041
CAACGCA8850.041.5819216
CGCAAGC8800.041.55681619
GGACTAA4050.041.4567877
Produced by FastQC (version 0.11.5)