FastQCFastQC Report
Thu 26 May 2016
SRR1544962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544962_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1223629
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT33320.27230475904052615No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT30850.2521189020528281Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT25980.2123192568989457No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.17268305997978145No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT17360.14187306773540018No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGA14620.11948065957900639No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA13430.1097554896132733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT351.0196345E-746.0000048
TCCGCGA206.311615E-446.020
TTAATCG1100.046.019
GACGTAT206.311615E-446.030
ATTCGAA253.417286E-546.026
CGATTGC206.311615E-446.09
TTATCGG253.417286E-546.01
ATCCGTC206.311615E-446.027
TACGCAC206.311615E-446.040
CTCGGTA206.311615E-446.031
TCGTACA301.8615028E-646.033
TTACGTC206.311615E-446.041
ACGTATA206.311615E-446.031
ACCCGTA253.417286E-546.032
ACTCCGA206.311615E-446.020
ATCGGGT206.311615E-446.03
ATGACGG1400.044.3571431
CAAACGG1150.044.0000041
TAATCGT1150.044.00000420
CTAGCGG2900.043.620691
Produced by FastQC (version 0.11.5)