SRR1544961_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544961_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	738777
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2972	0.40228648157698466	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	2567	0.34746615013732157	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2037	0.27572596331504634	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1539	0.20831725947071983	No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTTG	1420	0.19220955714647317	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.18828415069770715	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	760	0.1028727207262814	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	741	0.10030090270812438	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	6.309982E-4	46.0	24
TCCGCGT	20	6.309982E-4	46.0	20
TCGATCA	20	6.309982E-4	46.0	16
AATGCGG	115	0.0	46.0	1
ATAACGG	45	3.092282E-10	46.0	1
ATACCGG	25	3.4159617E-5	46.0	1
GCGATTG	70	0.0	45.999996	8
ATGACGG	70	0.0	45.999996	1
ACCACCC	35	1.0189069E-7	45.999996	21
TCGGCGT	135	0.0	44.2963	4
AACGGGA	135	0.0	44.2963	3
CTAGCGG	200	0.0	43.7	1
CTCGAGG	175	0.0	43.37143	1
CGTAGGG	310	0.0	43.032257	2
CAAACGG	75	0.0	42.93333	1
CGTTCAT	60	1.8189894E-12	42.166668	16
ACGATGG	60	1.8189894E-12	42.166668	1
ACGTAGG	60	1.8189894E-12	42.166668	1
GCGTAGG	120	0.0	42.166668	1
TAATCGT	60	1.8189894E-12	42.166668	20