FastQCFastQC Report
Thu 26 May 2016
SRR1544960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544960_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences456471
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT21620.47363359337175853TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT16630.36431668167309644TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT14440.31633992082739104TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.18007715714689432No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT7370.16145603992367533TruSeq Adapter, Index 20 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC6310.13823441138648457TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC5100.11172670333931399TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC4830.10581176022135032TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC4650.10186846480937453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.01776095E-746.00000424
ACCGGGT351.01776095E-746.0000043
TCCGCAA351.01776095E-746.00000430
GCGATGT351.01776095E-746.0000048
CGGTCCG351.01776095E-746.00000427
AATACGG351.01776095E-746.0000041
TACACGG351.01776095E-746.0000041
TATCACT206.307436E-446.039
TATCACC206.307436E-446.044
CGGGTAC301.8589199E-646.05
TCGTTTC405.5988494E-946.039
ACTATCA206.307436E-446.037
GATACGA206.307436E-446.08
ATCTCGG405.5988494E-946.01
TCGTTCA405.5988494E-946.015
CCCCTAC206.307436E-446.013
GCGACTA206.307436E-446.08
CGTATAT253.413896E-546.033
CACGGGT301.8589199E-646.03
CAAAACT253.413896E-546.023
Produced by FastQC (version 0.11.5)