##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544960_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	456471
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91786334728822	34.0	33.0	34.0	31.0	34.0
2	33.20998486212706	34.0	33.0	34.0	31.0	34.0
3	33.264571024227166	34.0	34.0	34.0	31.0	34.0
4	36.54849924748779	37.0	37.0	37.0	35.0	37.0
5	36.5340470698029	37.0	37.0	37.0	35.0	37.0
6	36.65354863726283	37.0	37.0	37.0	35.0	37.0
7	36.492465019683614	37.0	37.0	37.0	35.0	37.0
8	36.53061640279448	37.0	37.0	37.0	35.0	37.0
9	38.30421647815524	39.0	39.0	39.0	37.0	39.0
10	38.102679031088506	39.0	39.0	39.0	35.0	39.0
11	37.2312479872763	39.0	35.0	39.0	35.0	39.0
12	37.12250285341238	39.0	35.0	39.0	35.0	39.0
13	37.10063508963329	39.0	35.0	39.0	35.0	39.0
14	38.326918906129855	40.0	35.0	41.0	35.0	41.0
15	38.414197177914915	40.0	35.0	41.0	35.0	41.0
16	38.43911004203991	40.0	35.0	41.0	35.0	41.0
17	38.539214977512266	40.0	36.0	41.0	35.0	41.0
18	38.494066435764815	40.0	36.0	41.0	35.0	41.0
19	38.38566743560927	40.0	35.0	41.0	35.0	41.0
20	38.29633865020998	40.0	35.0	41.0	35.0	41.0
21	38.24269668828907	40.0	35.0	41.0	35.0	41.0
22	38.21407931719649	40.0	35.0	41.0	35.0	41.0
23	38.21509800184459	40.0	35.0	41.0	35.0	41.0
24	38.17517870795735	40.0	35.0	41.0	35.0	41.0
25	38.14576391490368	40.0	35.0	41.0	35.0	41.0
26	38.105432765717865	40.0	35.0	41.0	35.0	41.0
27	38.127705812636506	40.0	35.0	41.0	35.0	41.0
28	38.15070617848669	40.0	35.0	41.0	35.0	41.0
29	38.158095475944805	40.0	35.0	41.0	35.0	41.0
30	38.04366761524828	40.0	35.0	41.0	35.0	41.0
31	37.91948009840713	40.0	35.0	41.0	35.0	41.0
32	37.85411340479461	40.0	35.0	41.0	35.0	41.0
33	37.742651778535766	40.0	35.0	41.0	35.0	41.0
34	37.58132718179249	39.0	35.0	41.0	35.0	41.0
35	37.5501817201969	39.0	35.0	41.0	35.0	41.0
36	37.4307108228124	39.0	35.0	41.0	34.0	41.0
37	37.35573782343238	39.0	35.0	41.0	34.0	41.0
38	37.27994549489453	39.0	35.0	41.0	35.0	41.0
39	37.25268417927973	39.0	35.0	41.0	34.0	41.0
40	37.24081705080936	39.0	35.0	41.0	34.0	41.0
41	37.257674638695555	39.0	35.0	41.0	34.0	41.0
42	37.15686867292774	39.0	35.0	41.0	34.0	41.0
43	37.07524903005886	38.0	35.0	41.0	34.0	41.0
44	36.982881716472676	38.0	35.0	41.0	34.0	41.0
45	36.94243007770483	38.0	35.0	41.0	34.0	41.0
46	36.9491490149429	38.0	35.0	41.0	34.0	41.0
47	36.94764837196667	38.0	35.0	41.0	34.0	41.0
48	37.00242293595869	38.0	35.0	41.0	34.0	41.0
49	36.91320806798241	38.0	35.0	41.0	34.0	41.0
50	36.831787780603804	37.0	35.0	41.0	34.0	41.0
51	36.75013308622015	37.0	35.0	41.0	34.0	41.0
52	36.58350475714777	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	9.0
15	7.0
16	11.0
17	14.0
18	40.0
19	71.0
20	120.0
21	190.0
22	275.0
23	523.0
24	863.0
25	1575.0
26	2188.0
27	2513.0
28	2553.0
29	2682.0
30	3057.0
31	4016.0
32	5310.0
33	7621.0
34	23225.0
35	115168.0
36	14519.0
37	22527.0
38	41729.0
39	204401.0
40	1261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.00190592611579	21.84826637398652	26.028378582648187	22.121449117249508
2	24.887670848750524	24.449526914086547	28.308917762574183	22.353884474588746
3	25.78630405874634	25.745775744789924	26.466960661246823	22.000959535216914
4	25.905041065040272	26.828867551279274	24.84407552725146	22.422015856428995
5	23.27749188886041	35.59284160439546	21.203318502161146	19.926348004582987
6	87.38671240889344	2.714520747210666	7.1542332371607396	2.7445336067351485
7	86.99172565179387	2.0575239171820336	5.507031114791521	5.443719316232576
8	77.09514952757131	3.850627969794357	12.147759660526079	6.906462842108262
9	51.40786599805902	16.52635107159053	17.14172422782608	14.924058702524368
10	45.37046165035676	17.164726784395942	24.382271820115626	13.082539745131674
11	41.911972502086655	16.226660620280366	24.51722015199213	17.34414672564084
12	18.558462640562052	37.26304628333454	28.1816369495543	15.996854126549112
13	12.664988575396904	40.40059499946327	28.935025445209007	17.99939097993082
14	9.895042620451244	20.601746879867505	51.883690311104104	17.61952018857715
15	11.358662434196258	15.871544961235214	51.814682641394526	20.955109963174003
16	12.191793125959808	19.186541970902862	28.689007625895186	39.93265727724214
17	16.987497562824363	21.90894930893748	37.93340650337042	23.170146624867733
18	23.884321238370017	26.012824472967615	27.950077880084383	22.15277640857798
19	26.312076780343112	21.710031962600034	25.75957727873184	26.21831397832502
20	17.545473863618938	32.995086215772744	26.89918965279284	22.56025026781548
21	19.53442825502606	23.78004298191999	23.71716932729571	32.968359435758245
22	15.09318226130466	32.908771860643945	22.870456173557578	29.127589704493822
23	15.266468187464262	21.74048296605918	40.62382933417457	22.369219512301985
24	16.268284294073446	21.928227642062694	37.42822654670286	24.375261517161004
25	13.90405962262663	31.178103318721234	30.996492657803014	23.921344400849122
26	16.507072738465315	31.44580926280092	26.38984732874597	25.657270669987795
27	17.083670156483105	29.066030481673533	37.5730331171093	16.277266244734058
28	15.257048092868988	25.33392044620578	32.01101493851745	27.398016522407776
29	18.111336755237463	32.04058965410727	26.626883197399177	23.221190393256087
30	28.007255663558034	27.072913722887105	20.78708176423037	24.132748849324493
31	28.765901886428715	28.52601808220018	21.859219972353117	20.848860059017987
32	25.977334814259834	30.287356699549367	21.49906565805933	22.23624282813147
33	18.430086467705507	31.222574928089625	23.996267013676665	26.351071590528203
34	18.50522815250038	27.373699534033925	29.93640340788352	24.184668905582175
35	33.31449314414278	22.98678338821086	22.1878279233511	21.510895544295256
36	25.41081470673931	27.05911218894519	25.84041483467734	21.68965826963816
37	21.791307662480204	28.358647099158546	26.022682711497552	23.8273625268637
38	18.73656815000296	25.966600287860565	26.018082200183585	29.278749361952894
39	25.58716764044156	20.495277903744157	25.10038972902988	28.817164726784394
40	15.462975742161058	19.831489842728235	28.99483209229064	35.71070232282007
41	20.98468467876382	25.718610820840755	23.636112699382874	29.660591801012547
42	21.058731003722034	23.296989293952958	26.142076933693488	29.502202768631523
43	23.805674402097836	28.03726852308252	26.206045948154426	21.95101112666522
44	17.277548847571918	32.4920969787785	26.209989243566405	24.020364930083183
45	23.033226645285243	28.76918796593869	25.982592541475803	22.214992847300266
46	23.604128192152405	22.19461915433839	27.734730136197044	26.466522517312164
47	22.49759568515853	19.046335911810388	30.204328423930544	28.25173997910053
48	21.354259087652885	17.494649167197913	28.767435390200035	32.38365635494917
49	21.096849526037797	24.85108583020608	29.33789879313253	24.714165850623587
50	20.065020559904134	26.764460392883667	25.736355650194646	27.434163397017553
51	18.451993664438703	20.844478619671346	32.46361762302534	28.239910092864605
52	22.877466476512197	19.573423065211152	32.955215117718325	24.593895340558326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	114.5
2	139.0
3	780.5
4	1422.0
5	980.0
6	538.0
7	645.5
8	753.0
9	814.5
10	876.0
11	862.5
12	849.0
13	774.0
14	670.5
15	642.0
16	569.0
17	496.0
18	530.0
19	564.0
20	536.5
21	509.0
22	594.0
23	679.0
24	758.0
25	837.0
26	846.0
27	855.0
28	1298.0
29	1741.0
30	1917.5
31	2094.0
32	2683.0
33	3272.0
34	3741.5
35	4211.0
36	5245.5
37	6280.0
38	7484.0
39	10649.5
40	12611.0
41	19566.5
42	26522.0
43	35880.5
44	45239.0
45	51509.0
46	57779.0
47	60028.5
48	62278.0
49	61135.0
50	59992.0
51	52317.0
52	44642.0
53	37789.5
54	30937.0
55	24757.5
56	18578.0
57	16287.5
58	13997.0
59	13598.0
60	13199.0
61	11975.0
62	10751.0
63	9522.5
64	7033.5
65	5773.0
66	4624.0
67	3475.0
68	2869.0
69	2263.0
70	1826.5
71	1390.0
72	1137.5
73	885.0
74	874.5
75	864.0
76	675.5
77	487.0
78	298.0
79	109.0
80	112.5
81	116.0
82	71.0
83	26.0
84	26.0
85	26.0
86	14.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	456471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.214714638888967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81360280248865	17.603300725774904
2	12.317735894695335	6.211763911638853
3	4.612645131968966	3.489195921991735
4	2.22939822230391	2.248545599673578
5	1.200658783952289	1.5137134308016207
6	0.8006013386835277	1.211216057665059
7	0.5632732813682273	0.9941942537387326
8	0.441576128647552	0.8907372860154679
9	0.35899757586805237	0.8146819288409249
>10	6.73837440951919	45.639274325981454
>50	0.8005731191096679	12.917209962329345
>100	0.11647860799206895	4.719543261539625
>500	0.0034769733728975804	0.5917785169812375
>1k	0.0026077300296731857	1.1548448170274594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2162	0.47363359337175853	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	1663	0.36431668167309644	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1444	0.31633992082739104	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.18007715714689432	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	737	0.16145603992367533	TruSeq Adapter, Index 20 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	631	0.13823441138648457	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	510	0.11172670333931399	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	483	0.10581176022135032	TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	465	0.10186846480937453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.371546056595052	0.0
2	0.0	0.0	0.0	1.452447143411082	0.0
3	0.0	0.0	0.0	2.295874217639237	0.0
4	0.0	0.0	0.0	2.811350556771405	0.0
5	0.0	0.0	0.0	5.620729465836822	0.0
6	0.0	0.0	0.0	6.239388701582357	0.0
7	0.0	0.0	0.0	9.576731051917866	0.0
8	0.0	0.0	0.0	12.843532228772474	0.0
9	0.0	0.0	0.0	16.399727474472638	0.0
10	0.0	0.0	0.0	17.545254791651605	0.0
11	0.0	0.0	0.0	18.788488206260638	0.0
12	0.0	0.0	0.0	19.835652210107543	0.0
13	0.0	0.0	0.0	20.168422528484832	0.0
14	0.0	0.0	0.0	20.461978964709697	0.0
15	0.0	0.0	0.0	21.492055355104707	0.0
16	0.0	0.0	0.0	22.556306972403505	0.0
17	0.0	0.0	0.0	24.46530009573445	0.0
18	0.0	0.0	0.0	24.9834052984746	0.0
19	0.0	0.0	0.0	25.75979635069917	0.0
20	2.1907196733198824E-4	0.0	0.0	26.60322342492732	0.0
21	6.572159019959647E-4	0.0	0.0	27.291766618251764	0.0
22	6.572159019959647E-4	0.0	0.0	28.14855708248717	0.0
23	6.572159019959647E-4	0.0	0.0	28.7028091598371	0.0
24	8.76287869327953E-4	0.0	0.0	29.137447943023762	0.0
25	8.76287869327953E-4	0.0	0.0	29.596403714584277	0.0
26	8.76287869327953E-4	0.0	0.0	29.991609543651183	0.0
27	8.76287869327953E-4	0.0	0.0	30.431506054053816	0.0
28	8.76287869327953E-4	0.0	0.0	30.804585614420194	0.0
29	8.76287869327953E-4	0.0	0.0	31.43135051295701	0.0
30	8.76287869327953E-4	0.0	0.0	32.02722626410002	0.0
31	8.76287869327953E-4	0.0	0.0	32.44696815350811	0.0
32	8.76287869327953E-4	0.0	0.0	32.86846261865485	0.0
33	8.76287869327953E-4	0.0	0.0	33.25753443263647	0.0
34	8.76287869327953E-4	0.0	0.0	33.927237436770355	0.0
35	8.76287869327953E-4	0.0	0.0	34.35771385257771	0.0
36	8.76287869327953E-4	0.0	0.0	34.77657945411647	0.0
37	8.76287869327953E-4	0.0	0.0	35.17967187400733	0.0
38	8.76287869327953E-4	0.0	0.0	35.52251950288189	0.0
39	0.0010953598366599412	0.0	0.0	36.01345978167288	0.0
40	0.0013144318039919295	0.0	0.0	36.43604960665628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.01776095E-7	46.000004	24
ACCGGGT	35	1.01776095E-7	46.000004	3
TCCGCAA	35	1.01776095E-7	46.000004	30
GCGATGT	35	1.01776095E-7	46.000004	8
CGGTCCG	35	1.01776095E-7	46.000004	27
AATACGG	35	1.01776095E-7	46.000004	1
TACACGG	35	1.01776095E-7	46.000004	1
TATCACT	20	6.307436E-4	46.0	39
TATCACC	20	6.307436E-4	46.0	44
CGGGTAC	30	1.8589199E-6	46.0	5
TCGTTTC	40	5.5988494E-9	46.0	39
ACTATCA	20	6.307436E-4	46.0	37
GATACGA	20	6.307436E-4	46.0	8
ATCTCGG	40	5.5988494E-9	46.0	1
TCGTTCA	40	5.5988494E-9	46.0	15
CCCCTAC	20	6.307436E-4	46.0	13
GCGACTA	20	6.307436E-4	46.0	8
CGTATAT	25	3.413896E-5	46.0	33
CACGGGT	30	1.8589199E-6	46.0	3
CAAAACT	25	3.413896E-5	46.0	23
>>END_MODULE