FastQCFastQC Report
Thu 26 May 2016
SRR1544959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544959_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences261504
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT15950.6099333088595204TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT11430.43708700440528636TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT9810.3751376651982379TruSeq Adapter, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTTG7070.2703591532060695TruSeq Adapter, Index 20 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6380.24397332354380813No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.20037934410181107No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT4330.16558064121390112TruSeq Adapter, Index 20 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4130.15793257464512972No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC3740.14301884483602545TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC3440.13154674498286834TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC3090.11816262848751836TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC2950.11280898188937837TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT206.302469E-446.00000422
GAAATGC206.302469E-446.00000430
CGAACGT206.302469E-446.00000437
AATTTGG206.302469E-446.0000041
CTCGTCT206.302469E-446.00000435
CGCGGGT405.5824785E-946.0000043
GACGTCT206.302469E-446.00000419
AGAAGTA206.302469E-446.00000411
ATAGCGG405.5824785E-946.0000041
GCCGGTA206.302469E-446.00000432
CAACCGG206.302469E-446.0000041
CCTACAT206.302469E-446.00000417
ATTAACC206.302469E-446.00000420
GCCGCAT206.302469E-446.00000418
TCTCGGT206.302469E-446.00000411
AGCGCCA206.302469E-446.00000421
GAACGTT206.302469E-446.00000438
CGGGACG206.302469E-446.00000416
AGACAGT206.302469E-446.00000411
ATTGGGC206.302469E-446.0000043
Produced by FastQC (version 0.11.5)