##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544959_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	261504
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92646383994126	34.0	33.0	34.0	31.0	34.0
2	33.2666536649535	34.0	33.0	34.0	31.0	34.0
3	33.38669389378365	34.0	34.0	34.0	31.0	34.0
4	36.60177664586393	37.0	37.0	37.0	35.0	37.0
5	36.60456819016153	37.0	37.0	37.0	35.0	37.0
6	36.41779093245228	37.0	37.0	37.0	35.0	37.0
7	36.26656571218796	37.0	37.0	37.0	35.0	37.0
8	36.40280454601077	37.0	37.0	37.0	35.0	37.0
9	38.26272638277044	39.0	39.0	39.0	37.0	39.0
10	38.131057268722465	39.0	39.0	39.0	35.0	39.0
11	37.34714956558982	39.0	35.0	39.0	35.0	39.0
12	37.304691324033286	39.0	35.0	39.0	35.0	39.0
13	37.29865699951053	39.0	35.0	39.0	35.0	39.0
14	38.48476122736172	41.0	35.0	41.0	35.0	41.0
15	38.542637971120904	41.0	35.0	41.0	35.0	41.0
16	38.589872430249635	41.0	35.0	41.0	35.0	41.0
17	38.70649014929026	41.0	36.0	41.0	35.0	41.0
18	38.674349149535	40.0	36.0	41.0	35.0	41.0
19	38.58927205702398	40.0	35.0	41.0	35.0	41.0
20	38.50827138399413	40.0	35.0	41.0	35.0	41.0
21	38.46148816079295	40.0	35.0	41.0	35.0	41.0
22	38.42585581864905	40.0	35.0	41.0	35.0	41.0
23	38.405076021781696	40.0	35.0	41.0	35.0	41.0
24	38.382040045276554	40.0	35.0	41.0	35.0	41.0
25	38.34626238986784	40.0	35.0	41.0	35.0	41.0
26	38.29456910792952	40.0	35.0	41.0	35.0	41.0
27	38.379711209006366	40.0	35.0	41.0	35.0	41.0
28	38.401905898188936	40.0	35.0	41.0	35.0	41.0
29	38.38884682452276	40.0	36.0	41.0	35.0	41.0
30	38.297899840920216	40.0	35.0	41.0	35.0	41.0
31	38.186677832843856	40.0	35.0	41.0	35.0	41.0
32	38.12896934654919	40.0	35.0	41.0	35.0	41.0
33	38.04490944689183	40.0	35.0	41.0	35.0	41.0
34	37.879772393538914	40.0	35.0	41.0	35.0	41.0
35	37.86239216226138	40.0	35.0	41.0	35.0	41.0
36	37.77975862701909	40.0	35.0	41.0	35.0	41.0
37	37.70201603034753	40.0	35.0	41.0	35.0	41.0
38	37.67202413729809	40.0	35.0	41.0	35.0	41.0
39	37.654142957660305	40.0	35.0	41.0	35.0	41.0
40	37.6535081681351	40.0	35.0	41.0	35.0	41.0
41	37.628368973323546	40.0	35.0	41.0	35.0	41.0
42	37.57268340063632	40.0	35.0	41.0	35.0	41.0
43	37.51672249755262	39.0	35.0	41.0	35.0	41.0
44	37.30044282305433	39.0	35.0	41.0	35.0	41.0
45	37.32547112090064	39.0	35.0	41.0	35.0	41.0
46	37.28097084557024	39.0	35.0	41.0	35.0	41.0
47	37.253866097650516	39.0	35.0	41.0	35.0	41.0
48	37.25188142437592	39.0	35.0	41.0	35.0	41.0
49	37.177844315956925	39.0	35.0	41.0	35.0	41.0
50	37.10442287689672	38.0	35.0	41.0	35.0	41.0
51	37.033024351443956	38.0	35.0	41.0	34.0	41.0
52	36.58695086882036	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	5.0
17	9.0
18	21.0
19	38.0
20	62.0
21	93.0
22	125.0
23	262.0
24	445.0
25	769.0
26	1094.0
27	1355.0
28	1250.0
29	1245.0
30	1331.0
31	1625.0
32	2229.0
33	3590.0
34	11863.0
35	63314.0
36	7753.0
37	12644.0
38	24253.0
39	125571.0
40	553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.60910731767009	21.41458639255996	24.964436490455213	24.011869799314734
2	24.728876040137056	24.739583333333336	28.087142070484582	22.44439855604503
3	25.890617351933432	25.751040137053355	26.36976872246696	21.988573788546255
4	25.734596793930493	26.663072075379347	25.164433431228584	22.437897699461576
5	23.29830518844836	35.076710107684775	21.16105298580519	20.463931718061673
6	86.05183859520314	2.715828438570729	7.871772515907978	3.3605604503181596
7	85.69735070974059	1.967847528144885	6.15898800783162	6.175813754282918
8	74.88221977484092	4.01064610866373	13.416620778267255	7.6905133382280955
9	50.607638888888886	15.938953132648068	17.63414708761625	15.819260890846795
10	43.99626774351444	16.771062775330396	26.07914219285365	13.153527288301516
11	40.58025881057269	16.250994248653942	24.74302496328928	18.42572197748409
12	17.934333700440526	35.876697870778266	29.446968306412142	16.742000122369067
13	12.602866495349977	38.84070606950563	29.546010768477725	19.010416666666664
14	9.95281142927068	20.653986172295642	50.7177710474792	18.67543135095448
15	11.341317914831132	15.71371757219775	51.32885156632403	21.616112946647085
16	12.255644273127754	20.00466532060695	28.968199339207047	38.77149106705825
17	16.693052496328928	22.201954845814978	37.811658712677435	23.29333394517866
18	23.37593306412139	26.845096059716106	27.83590308370044	21.943067792462063
19	25.58087065589819	21.90750428291728	25.839375305922662	26.672249755261866
20	17.201648923152227	33.610575746451296	26.48487212432697	22.702903206069504
21	19.17026125795399	23.87114537444934	23.88185266764562	33.07674069995105
22	15.274336147821831	33.353600709740576	22.61686245717083	28.755200685266765
23	15.244508688203624	22.304056534508078	39.94508688203622	22.50634789525208
24	16.533972711698482	22.705962432697014	36.022011135584926	24.73805372001958
25	14.086591409691628	31.312331742535488	30.85918379833578	23.741893049437103
26	17.06207170827215	30.172387420460105	26.317379466470875	26.448161404796867
27	18.007755139500734	28.83627019089574	36.776875305922665	16.37909936368086
28	16.08809043073911	25.126193098384732	31.68402777777778	27.101688693098385
29	18.80391886930984	31.745212310327947	26.09596793930494	23.35490088105727
30	27.975480298580518	26.91507586882036	20.02837432697014	25.081069505628978
31	28.703193832599116	28.84659508076358	20.84480543318649	21.605405653450806
32	25.49215308370044	30.25039769946158	21.453973935389133	22.803475281448847
33	18.152686000978953	31.54712738619677	23.75336514929026	26.54682146353402
34	19.141963411649535	27.447763705335294	29.853463044542337	23.556809838472834
35	32.95551884483602	23.08148250122369	22.032550171316693	21.930448482623593
36	25.71509422418013	26.557146353401862	25.590430739109156	22.13732868330886
37	22.0405806412139	27.59575379344102	26.50360988742046	23.86005567792462
38	18.615011625061186	25.536511869799316	25.637848751835534	30.210627753303964
39	25.591577949094468	20.122827949094468	25.13422356828194	29.151370533529125
40	15.61429270680372	19.32016336270191	28.823650881057265	36.2418930494371
41	21.148816079295155	25.87799804209496	23.23406142927068	29.739124449339204
42	20.896429882525698	22.97211514929026	26.217572197748407	29.913882770435634
43	24.592740455212923	27.65540871267744	25.893676578560942	21.858174253548704
44	17.41005873715125	31.19378671071953	27.107424743024964	24.28872980910426
45	22.949170949583944	28.10932146353402	26.149122001957902	22.792385584924133
46	23.926211453744493	21.320132770435634	27.919267009300047	26.834388766519822
47	22.377095570239845	18.239491556534507	30.533758565834557	28.84965430739109
48	20.99470753793441	16.971442119432208	28.436276309348997	33.59757403328438
49	20.76450073421439	23.639408957415565	29.908911527165934	25.68717878120411
50	20.109826235927557	25.935740944689183	26.2630781938326	27.691354625550662
51	18.5090859030837	20.193954968184045	32.28975465002447	29.00720447870778
52	22.87001346059716	19.11748959862947	33.1119218061674	24.90057513460597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	64.0
2	87.0
3	488.0
4	889.0
5	628.5
6	368.0
7	428.0
8	488.0
9	488.0
10	488.0
11	494.5
12	501.0
13	478.5
14	423.5
15	391.0
16	350.5
17	310.0
18	320.5
19	331.0
20	319.5
21	308.0
22	345.0
23	382.0
24	424.0
25	466.0
26	455.5
27	445.0
28	675.5
29	906.0
30	996.5
31	1087.0
32	1363.0
33	1639.0
34	1880.0
35	2121.0
36	2703.5
37	3286.0
38	3982.5
39	5657.5
40	6636.0
41	10170.0
42	13704.0
43	19035.0
44	24366.0
45	28054.0
46	31742.0
47	34239.0
48	36736.0
49	36278.0
50	35820.0
51	31318.0
52	26816.0
53	22559.0
54	18302.0
55	14670.5
56	11039.0
57	9587.0
58	8135.0
59	7867.5
60	7600.0
61	6959.0
62	6318.0
63	5790.5
64	4440.5
65	3618.0
66	2847.0
67	2076.0
68	1698.0
69	1320.0
70	1069.5
71	819.0
72	671.0
73	523.0
74	529.0
75	535.0
76	414.0
77	293.0
78	176.0
79	59.0
80	62.0
81	65.0
82	42.0
83	19.0
84	24.5
85	30.0
86	15.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	261504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.48748776309349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92760203697665	21.92891886930984
2	10.485914256327924	6.393783651492903
3	3.470636931490354	3.174330029368576
4	1.8250006271479817	2.2255873715124816
5	1.0824574166520333	1.6500703622124326
6	0.8102751925344305	1.4821953010279003
7	0.6748112284574668	1.4401309348996574
8	0.6108421343100118	1.4898433675966716
9	0.5280586007074229	1.4489262114537445
>10	8.31723653513283	49.89751590797846
>50	0.20946742593382336	4.084449951052374
>100	0.050171838547023555	2.6473782427802255
>500	0.0050171838547023555	1.0898494860499266
>1k	0.0025085919273511777	1.0470203132648066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1595	0.6099333088595204	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	1143	0.43708700440528636	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	981	0.3751376651982379	TruSeq Adapter, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTTG	707	0.2703591532060695	TruSeq Adapter, Index 20 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	638	0.24397332354380813	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.20037934410181107	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	433	0.16558064121390112	TruSeq Adapter, Index 20 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	413	0.15793257464512972	No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	374	0.14301884483602545	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	344	0.13154674498286834	TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	309	0.11816262848751836	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	295	0.11280898188937837	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44205824767498775	0.0
2	0.0	0.0	0.0	1.713166911404797	0.0
3	0.0	0.0	0.0	2.6515846793930495	0.0
4	0.0	0.0	0.0	3.229013705335291	0.0
5	0.0	0.0	0.0	6.38919481155164	0.0
6	0.0	0.0	0.0	7.020925110132159	0.0
7	0.0	0.0	0.0	10.767330518844837	0.0
8	0.0	0.0	0.0	14.302266886930983	0.0
9	0.0	0.0	0.0	18.214252936857562	0.0
10	0.0	0.0	0.0	19.548458149779737	0.0
11	0.0	0.0	0.0	20.815742780225158	0.0
12	0.0	0.0	0.0	21.953775085658346	0.0
13	0.0	0.0	0.0	22.304438937836515	0.0
14	0.0	0.0	0.0	22.58665259422418	0.0
15	0.0	0.0	0.0	23.652410670582476	0.0
16	0.0	0.0	0.0	24.762909936368086	0.0
17	0.0	0.0	0.0	26.496726627508565	0.0
18	0.0	0.0	0.0	27.031708883994128	0.0
19	0.0	0.0	0.0	27.800339574155654	0.0
20	0.0	0.0	0.0	28.66227667645619	0.0
21	0.0011472099853157122	0.0	0.0	29.367810817425354	0.0
22	0.0011472099853157122	0.0	0.0	30.215216593245227	0.0
23	0.0011472099853157122	0.0	0.0	30.73604992657856	0.0
24	0.0011472099853157122	0.0	0.0	31.17734336759667	0.0
25	0.0011472099853157122	0.0	0.0	31.65687714145864	0.0
26	0.0011472099853157122	0.0	0.0	32.06222466960352	0.0
27	0.0011472099853157122	0.0	0.0	32.508489353891335	0.0
28	0.0011472099853157122	0.0	0.0	32.8878334557024	0.0
29	0.0011472099853157122	0.0	0.0	33.48055861478218	0.0
30	0.0011472099853157122	0.0	0.0	34.03810266764562	0.0
31	0.0011472099853157122	0.0	0.0	34.44689182574645	0.0
32	0.0011472099853157122	0.0	0.0	34.866005873715125	0.0
33	0.0011472099853157122	0.0	0.0	35.235789892315225	0.0
34	0.0011472099853157122	0.0	0.0	35.84381118453255	0.0
35	0.0011472099853157122	0.0	0.0	36.261778022515905	0.0
36	0.0011472099853157122	0.0	0.0	36.66597834067548	0.0
37	0.0011472099853157122	0.0	0.0	37.05029368575624	0.0
38	0.0011472099853157122	0.0	0.0	37.39254466470876	0.0
39	0.0011472099853157122	0.0	0.0	37.8311612824278	0.0
40	0.002676823299069995	0.0	0.0	38.235361600587375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGT	20	6.302469E-4	46.000004	22
GAAATGC	20	6.302469E-4	46.000004	30
CGAACGT	20	6.302469E-4	46.000004	37
AATTTGG	20	6.302469E-4	46.000004	1
CTCGTCT	20	6.302469E-4	46.000004	35
CGCGGGT	40	5.5824785E-9	46.000004	3
GACGTCT	20	6.302469E-4	46.000004	19
AGAAGTA	20	6.302469E-4	46.000004	11
ATAGCGG	40	5.5824785E-9	46.000004	1
GCCGGTA	20	6.302469E-4	46.000004	32
CAACCGG	20	6.302469E-4	46.000004	1
CCTACAT	20	6.302469E-4	46.000004	17
ATTAACC	20	6.302469E-4	46.000004	20
GCCGCAT	20	6.302469E-4	46.000004	18
TCTCGGT	20	6.302469E-4	46.000004	11
AGCGCCA	20	6.302469E-4	46.000004	21
GAACGTT	20	6.302469E-4	46.000004	38
CGGGACG	20	6.302469E-4	46.000004	16
AGACAGT	20	6.302469E-4	46.000004	11
ATTGGGC	20	6.302469E-4	46.000004	3
>>END_MODULE