FastQCFastQC Report
Thu 26 May 2016
SRR1544958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544958_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences460573
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT22440.48721918132413317No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT19300.4190432352743213Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT15230.3306750504263168No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.23883293202163394No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT7340.15936670191261754No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC7130.15480716411947726No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC5760.12506160804041921No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC5640.12245615787291049No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC5380.11681101584330822No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT4710.1022639190747178No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG4650.10096119399096344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGC351.01777914E-746.00000419
CGCGGCA351.01777914E-746.00000425
TAACCGG351.01777914E-746.0000041
CGCGTGC351.01777914E-746.00000445
AACTAAC351.01777914E-746.00000412
TATACGG351.01777914E-746.0000041
ATCGCCG351.01777914E-746.00000421
GCGTAGG551.8189894E-1246.0000041
GCGAGAC206.307497E-446.020
TGATCCC206.307497E-446.043
TCACGAC206.307497E-446.024
CGGGTCA405.5988494E-946.05
CGTGGAC253.4139448E-546.013
CTGTCGG301.8589562E-646.01
CTCACGA206.307497E-446.023
CCCCTAG206.307497E-446.010
CCCTCGC253.4139448E-546.026
GTTTAGC206.307497E-446.030
CTATAGG253.4139448E-546.01
ATTACGG206.307497E-446.032
Produced by FastQC (version 0.11.5)