##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544958_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	460573
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87677089191073	34.0	33.0	34.0	31.0	34.0
2	33.181708437099005	34.0	33.0	34.0	31.0	34.0
3	33.22682180674942	34.0	33.0	34.0	31.0	34.0
4	36.533279197868744	37.0	37.0	37.0	35.0	37.0
5	36.52666352565174	37.0	37.0	37.0	35.0	37.0
6	36.641366298067844	37.0	37.0	37.0	35.0	37.0
7	36.45816189833099	37.0	37.0	37.0	35.0	37.0
8	36.493806627830985	37.0	37.0	37.0	35.0	37.0
9	38.26950125170168	39.0	39.0	39.0	37.0	39.0
10	38.120632342755655	39.0	39.0	39.0	35.0	39.0
11	37.494937827445376	39.0	35.0	39.0	35.0	39.0
12	37.4132873616126	39.0	35.0	39.0	35.0	39.0
13	37.39774368015494	39.0	35.0	39.0	35.0	39.0
14	38.71622739500579	41.0	35.0	41.0	35.0	41.0
15	38.779739585255754	41.0	35.0	41.0	35.0	41.0
16	38.79191137995497	41.0	35.0	41.0	35.0	41.0
17	38.83607376029424	41.0	36.0	41.0	35.0	41.0
18	38.81858033362789	41.0	36.0	41.0	35.0	41.0
19	38.73369042475351	41.0	36.0	41.0	35.0	41.0
20	38.64792769007302	41.0	35.0	41.0	35.0	41.0
21	38.58788509096278	41.0	35.0	41.0	35.0	41.0
22	38.562514519956665	40.0	35.0	41.0	35.0	41.0
23	38.56293573440041	40.0	35.0	41.0	35.0	41.0
24	38.520692702351205	40.0	35.0	41.0	35.0	41.0
25	38.485273778532395	40.0	35.0	41.0	35.0	41.0
26	38.445838119038676	40.0	35.0	41.0	35.0	41.0
27	38.42598458876226	40.0	35.0	41.0	35.0	41.0
28	38.44471994667512	40.0	36.0	41.0	35.0	41.0
29	38.45451209688801	40.0	36.0	41.0	35.0	41.0
30	38.3864555673042	40.0	35.0	41.0	35.0	41.0
31	38.32122595115215	40.0	35.0	41.0	35.0	41.0
32	38.22197566943785	40.0	35.0	41.0	35.0	41.0
33	38.12804267727375	40.0	35.0	41.0	35.0	41.0
34	38.05189188250288	40.0	35.0	41.0	35.0	41.0
35	37.974110510168856	40.0	35.0	41.0	35.0	41.0
36	37.940545798385926	40.0	35.0	41.0	35.0	41.0
37	37.870684994561124	40.0	35.0	41.0	35.0	41.0
38	37.79689864581727	40.0	35.0	41.0	35.0	41.0
39	37.71424942408695	40.0	35.0	41.0	35.0	41.0
40	37.637386472936974	40.0	35.0	41.0	34.0	41.0
41	37.60169397685058	40.0	35.0	41.0	34.0	41.0
42	37.4964272764578	40.0	35.0	41.0	34.0	41.0
43	37.4056295093286	39.0	35.0	41.0	34.0	41.0
44	37.33696721258085	39.0	35.0	41.0	34.0	41.0
45	37.300034956456415	39.0	35.0	41.0	34.0	41.0
46	37.31504669183821	39.0	35.0	41.0	34.0	41.0
47	37.25798081954435	39.0	35.0	41.0	34.0	41.0
48	37.29002134297929	39.0	35.0	41.0	34.0	41.0
49	37.208218892553404	39.0	35.0	41.0	34.0	41.0
50	37.11209732224859	39.0	35.0	41.0	34.0	41.0
51	37.03674770340424	39.0	35.0	41.0	34.0	41.0
52	36.834137042336394	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	8.0
16	6.0
17	18.0
18	35.0
19	64.0
20	104.0
21	178.0
22	300.0
23	489.0
24	890.0
25	1622.0
26	2319.0
27	2610.0
28	2561.0
29	2874.0
30	3278.0
31	4119.0
32	5297.0
33	7436.0
34	20091.0
35	87726.0
36	16219.0
37	24093.0
38	44764.0
39	231806.0
40	1662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.854885110503655	22.257926539332527	25.12327036105026	20.763917989113562
2	27.214795483017895	24.077616360490083	25.86365245031732	22.8439357061747
3	25.986325729037524	25.23791016842064	26.03018414019059	22.745579962351243
4	25.677797005035032	27.492493046704862	23.21043569640426	23.619274251855842
5	23.56021738139231	35.82537404493967	20.72635608253198	19.88805249113604
6	86.1659280939178	2.6137007597058446	8.225840420519658	2.9945307258567047
7	85.66372757413049	2.4632360125322155	6.19228656477909	5.680749848558209
8	74.21537953809711	4.724115395387919	13.535964982749748	7.524540083765223
9	46.15229290470783	19.033030594498594	17.98108008936694	16.833596411426637
10	39.31146636906636	18.93662893830077	26.752110957437804	14.999793735195071
11	36.27481419883493	16.957138173536006	26.95577031219807	19.812277315430997
12	20.219596024951528	31.297753016351372	30.426447056166992	18.05620390253011
13	14.472190076274554	34.722834382388896	30.92473940070304	19.880236140633514
14	12.639473004279452	22.979636235732446	45.850712047818696	18.530178712169405
15	14.602679705497282	18.502170122868687	45.71761696842846	21.177533203205574
16	15.3387193778185	21.516893087523584	29.43203357556782	33.712353959090095
17	18.25682356542828	22.406654319727817	35.12429082903253	24.21223128581137
18	22.499364921521668	27.686381963336977	28.873815877179076	20.94043723796228
19	24.218962032077435	23.064530487023774	27.41997468370921	25.296532797189585
20	19.456199125871468	30.447724899201646	28.12778864588241	21.968287329044472
21	19.632067012178307	24.017908127484677	27.054777418563397	29.295247441773615
22	16.338778000447267	29.467424273676485	25.061173798724635	29.132623927151613
23	16.88114587698367	22.630505913286274	37.376702498843834	23.111645710886222
24	18.97332236149318	22.197784064632533	34.14637853282759	24.682515041046695
25	15.191511443354255	29.327598448020183	30.16850748958363	25.312382619041934
26	17.75440592479368	27.28731384601355	28.147329522138726	26.810950707054037
27	19.565628032906833	25.78462046190289	34.470974199529714	20.17877730566056
28	17.3056171334403	24.018776610873847	30.062986757799525	28.612619497886328
29	18.36429838483802	26.521962859307862	27.380458689501992	27.73328006635213
30	27.123170485460502	25.062476523808385	22.94012024152523	24.87423274920588
31	25.52711513701411	25.23313350978021	22.270736669322776	26.969014683882904
32	23.95298899414425	26.08185890184618	25.14715365425242	24.81799844975715
33	18.806573550772622	26.452484188174296	25.620911343044423	29.120030918008656
34	18.707783565254584	24.658414626997242	29.528869473460233	27.10493233428794
35	24.05351594644063	26.009774780545104	26.581888213160564	23.354821059853702
36	19.569536208158098	29.690190262998485	29.709731139254796	21.03054238958862
37	18.60313131685965	33.26790758468256	26.12550019215195	22.00346090630584
38	16.74349125980029	30.660720450395484	28.09413491455209	24.50165337525213
39	22.590338556537183	25.661730062335398	28.884454798696407	22.863476582431016
40	15.791633465270435	22.41251658260471	32.827803627220874	28.96804632490398
41	20.967143102179243	25.05813410686254	25.434404535220256	28.54031825573796
42	22.319154618268982	20.86183949124243	29.874308741502432	26.94469714898615
43	22.09573726640511	24.87141017819108	28.51100694135349	24.521845614050324
44	16.968428457595213	29.824370946625184	28.207254875991428	24.999945719788176
45	22.41751036209244	27.873323012855728	25.720135570257046	23.989031054794786
46	22.253149880692096	23.832486923896973	28.19010232905533	25.724260866355603
47	20.344223391297362	21.70861079568277	32.16862473484117	25.7785410781787
48	20.434328542923705	19.47986529822634	29.73882533279198	30.346980826057973
49	20.405234349386525	23.61297774728436	31.709848384512334	24.27193951881678
50	19.07797460988812	24.994951940300453	29.164323570856304	26.762749878955127
51	18.841964248881286	21.091770468525077	31.79539399834554	28.270871284248102
52	20.245433405779323	19.330486155289172	35.68402837335232	24.740052065579178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	145.0
2	202.0
3	1103.0
4	2004.0
5	1352.5
6	701.0
7	783.5
8	866.0
9	895.0
10	924.0
11	917.5
12	911.0
13	844.0
14	754.0
15	731.0
16	724.0
17	717.0
18	742.5
19	768.0
20	819.5
21	871.0
22	801.0
23	731.0
24	837.0
25	943.0
26	1101.0
27	1259.0
28	1941.5
29	2624.0
30	2858.5
31	3093.0
32	3832.5
33	4572.0
34	5148.5
35	5725.0
36	6335.0
37	6945.0
38	7987.5
39	11482.5
40	13935.0
41	20542.0
42	27149.0
43	37479.5
44	47810.0
45	56954.5
46	66099.0
47	65450.0
48	64801.0
49	56303.0
50	47805.0
51	41458.5
52	35112.0
53	30126.0
54	25140.0
55	22362.5
56	19585.0
57	18102.0
58	16619.0
59	15533.5
60	14448.0
61	13163.0
62	11878.0
63	10394.0
64	7492.0
65	6074.0
66	5026.5
67	3979.0
68	3271.5
69	2564.0
70	2133.0
71	1702.0
72	1368.5
73	1035.0
74	827.5
75	620.0
76	519.5
77	419.0
78	313.5
79	208.0
80	168.0
81	128.0
82	90.5
83	53.0
84	34.5
85	16.0
86	9.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	460573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.52012058827985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.23788480576889	20.734308293320066
2	11.74787572502193	6.935974161175458
3	4.090774572872145	3.622804760719642
4	1.9716708565018555	2.328158457773272
5	1.1927589158716965	1.7605193514639208
6	0.7933608666950339	1.405206507291577
7	0.6171139112559478	1.2752093953886428
8	0.5513331819671718	1.3020337612792758
9	0.4761788027817731	1.2651170111730603
>10	7.992092869034267	48.81391445284876
>50	0.2560995778429517	4.971845671387465
>100	0.06623264944214266	3.429413430416959
>500	0.0036795916356745927	0.6788874300046241
>1k	0.0029436733085396742	1.4766073157572577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2244	0.48721918132413317	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	1930	0.4190432352743213	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1523	0.3306750504263168	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.23883293202163394	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	734	0.15936670191261754	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	713	0.15480716411947726	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	576	0.12506160804041921	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	564	0.12245615787291049	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	538	0.11681101584330822	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	471	0.1022639190747178	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	465	0.10096119399096344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4281623108606019	0.0
2	0.0	0.0	0.0	1.5274451607019952	0.0
3	0.0	0.0	0.0	2.491244601832934	0.0
4	0.0	0.0	0.0	3.102656907808317	0.0
5	0.0	0.0	0.0	6.234625130001107	0.0
6	0.0	0.0	0.0	6.850814094616923	0.0
7	0.0	0.0	0.0	9.932193159390586	0.0
8	0.0	0.0	0.0	13.146015941012609	0.0
9	0.0	0.0	0.0	16.31619743232886	0.0
10	2.1712084729239448E-4	0.0	0.0	17.639548996576004	0.0
11	2.1712084729239448E-4	0.0	0.0	18.996988533848054	0.0
12	2.1712084729239448E-4	0.0	0.0	20.093883054369233	0.0
13	2.1712084729239448E-4	0.0	0.0	20.47840407492406	0.0
14	2.1712084729239448E-4	0.0	0.0	20.788018403163015	0.0
15	2.1712084729239448E-4	0.0	0.0	21.867977497595387	0.0
16	2.1712084729239448E-4	0.0	0.0	23.021974800954464	0.0
17	2.1712084729239448E-4	0.0	0.0	24.74287463659398	0.0
18	2.1712084729239448E-4	0.0	0.0	25.410304121170803	0.0
19	2.1712084729239448E-4	0.0	0.0	26.38756505483387	0.0
20	2.1712084729239448E-4	0.0	0.0	27.359397967314628	0.0
21	2.1712084729239448E-4	0.0	0.0	28.180766132621756	0.0
22	2.1712084729239448E-4	0.0	0.0	29.080297802954146	0.0
23	2.1712084729239448E-4	0.0	0.0	29.69909221773747	0.0
24	2.1712084729239448E-4	0.0	0.0	30.231689656145715	0.0
25	4.3424169458478897E-4	0.0	0.0	30.781439641490056	0.0
26	4.3424169458478897E-4	0.0	0.0	31.273869723149208	0.0
27	4.3424169458478897E-4	0.0	0.0	31.801473382069727	0.0
28	4.3424169458478897E-4	0.0	0.0	32.28435014644801	0.0
29	4.3424169458478897E-4	0.0	0.0	32.96784657372447	0.0
30	4.3424169458478897E-4	0.0	0.0	33.59272037223198	0.0
31	4.3424169458478897E-4	0.0	0.0	34.0803737952507	0.0
32	6.513625418771834E-4	0.0	0.0	34.56998130589505	0.0
33	6.513625418771834E-4	0.0	0.0	35.05611488298272	0.0
34	6.513625418771834E-4	0.0	0.0	35.731143597214775	0.0
35	6.513625418771834E-4	0.0	0.0	36.22639624988872	0.0
36	8.684833891695779E-4	0.0	0.0	36.69928545529156	0.0
37	8.684833891695779E-4	0.0	0.0	37.17195753984711	0.0
38	8.684833891695779E-4	0.0	0.0	37.63594479051095	0.0
39	8.684833891695779E-4	0.0	0.0	38.14487605656432	0.0
40	8.684833891695779E-4	0.0	0.0	38.67009138616463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGC	35	1.01777914E-7	46.000004	19
CGCGGCA	35	1.01777914E-7	46.000004	25
TAACCGG	35	1.01777914E-7	46.000004	1
CGCGTGC	35	1.01777914E-7	46.000004	45
AACTAAC	35	1.01777914E-7	46.000004	12
TATACGG	35	1.01777914E-7	46.000004	1
ATCGCCG	35	1.01777914E-7	46.000004	21
GCGTAGG	55	1.8189894E-12	46.000004	1
GCGAGAC	20	6.307497E-4	46.0	20
TGATCCC	20	6.307497E-4	46.0	43
TCACGAC	20	6.307497E-4	46.0	24
CGGGTCA	40	5.5988494E-9	46.0	5
CGTGGAC	25	3.4139448E-5	46.0	13
CTGTCGG	30	1.8589562E-6	46.0	1
CTCACGA	20	6.307497E-4	46.0	23
CCCCTAG	20	6.307497E-4	46.0	10
CCCTCGC	25	3.4139448E-5	46.0	26
GTTTAGC	20	6.307497E-4	46.0	30
CTATAGG	25	3.4139448E-5	46.0	1
ATTACGG	20	6.307497E-4	46.0	32
>>END_MODULE