FastQCFastQC Report
Thu 26 May 2016
SRR1544957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544957_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences296022
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT19160.6472491909385114No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT16340.5519860010404631Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT12850.43408935822337524No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTG9720.32835397369114455Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.2540351730614616No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6490.21924046185756463No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT4930.16654167595651673No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC4410.14897541398950076No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC4380.147961975799096No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC4360.14728635033882617No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT4110.13884103208545312No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC3930.13276040294302452No Hit
CTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTGAAA3460.11688320462668315Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG351.01612386E-746.0000044
ACTAAGG351.01612386E-746.0000041
GCGATAG351.01612386E-746.0000048
TGCTAGG351.01612386E-746.0000041
ACAACGG206.3038245E-446.01
CTATGTT206.3038245E-446.010
TTGGCGT206.3038245E-446.023
CCTTACT206.3038245E-446.046
TCGTTCA253.4109675E-546.015
CTCGTTC253.4109675E-546.014
TCCGCCG206.3038245E-446.015
TCCGCAA206.3038245E-446.031
CACGGGT301.856688E-646.03
GGATGCC253.4109675E-546.07
TAGACGG600.046.01
CCCTAGG206.3038245E-446.01
AACCCGG206.3038245E-446.01
GGCGTTT206.3038245E-446.036
CAGTTGG253.4109675E-546.01
CTATAGG253.4109675E-546.01
Produced by FastQC (version 0.11.5)