##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544957_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	296022
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87560384025512	34.0	33.0	34.0	31.0	34.0
2	33.231175385613234	34.0	33.0	34.0	31.0	34.0
3	33.35329806568431	34.0	34.0	34.0	31.0	34.0
4	36.59376330137625	37.0	37.0	37.0	35.0	37.0
5	36.59522940862504	37.0	37.0	37.0	35.0	37.0
6	36.38811304565201	37.0	37.0	37.0	35.0	37.0
7	36.19850889460918	37.0	37.0	37.0	35.0	37.0
8	36.34616008269656	37.0	37.0	37.0	35.0	37.0
9	38.22175378856977	39.0	39.0	39.0	37.0	39.0
10	38.15155630324773	39.0	39.0	39.0	35.0	39.0
11	37.62065319469499	39.0	37.0	39.0	35.0	39.0
12	37.60505300281736	39.0	37.0	39.0	35.0	39.0
13	37.59687117849349	39.0	37.0	39.0	35.0	39.0
14	38.873897885967935	41.0	37.0	41.0	35.0	41.0
15	38.90828046564107	41.0	37.0	41.0	35.0	41.0
16	38.948841640148366	41.0	37.0	41.0	35.0	41.0
17	39.016171095391556	41.0	37.0	41.0	35.0	41.0
18	39.01163764855315	41.0	37.0	41.0	35.0	41.0
19	38.96172243954841	41.0	37.0	41.0	35.0	41.0
20	38.88702528866098	41.0	36.0	41.0	35.0	41.0
21	38.82979981217612	41.0	35.0	41.0	35.0	41.0
22	38.807646053333876	41.0	35.0	41.0	35.0	41.0
23	38.782212132882016	41.0	35.0	41.0	35.0	41.0
24	38.765017464918145	41.0	35.0	41.0	35.0	41.0
25	38.725402166055225	41.0	35.0	41.0	35.0	41.0
26	38.67803744316301	41.0	35.0	41.0	35.0	41.0
27	38.75260960334029	41.0	36.0	41.0	35.0	41.0
28	38.747359993513996	41.0	36.0	41.0	35.0	41.0
29	38.75871725750113	41.0	36.0	41.0	35.0	41.0
30	38.68956023538791	41.0	36.0	41.0	35.0	41.0
31	38.61935599381127	41.0	35.0	41.0	35.0	41.0
32	38.54843221111945	41.0	35.0	41.0	35.0	41.0
33	38.46557012654465	41.0	35.0	41.0	35.0	41.0
34	38.37352967009209	41.0	35.0	41.0	35.0	41.0
35	38.30743660944119	40.0	35.0	41.0	35.0	41.0
36	38.27596259737452	40.0	35.0	41.0	35.0	41.0
37	38.212906473167536	40.0	35.0	41.0	35.0	41.0
38	38.17311551168494	40.0	35.0	41.0	35.0	41.0
39	38.11578193512644	40.0	35.0	41.0	35.0	41.0
40	38.06478572538528	40.0	35.0	41.0	35.0	41.0
41	38.03916938605914	40.0	35.0	41.0	35.0	41.0
42	37.9762213619258	40.0	35.0	41.0	35.0	41.0
43	37.907496740107156	40.0	35.0	41.0	35.0	41.0
44	37.76450736769564	40.0	35.0	41.0	35.0	41.0
45	37.75998405523914	40.0	35.0	41.0	35.0	41.0
46	37.70793049165265	40.0	35.0	41.0	35.0	41.0
47	37.626500733053625	40.0	35.0	41.0	35.0	41.0
48	37.600539824742754	40.0	35.0	41.0	35.0	41.0
49	37.53704116585929	39.0	35.0	41.0	35.0	41.0
50	37.44234212322057	39.0	35.0	41.0	35.0	41.0
51	37.35349737519509	39.0	35.0	41.0	35.0	41.0
52	36.918897919749206	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	3.0
16	7.0
17	6.0
18	9.0
19	32.0
20	49.0
21	105.0
22	169.0
23	255.0
24	496.0
25	855.0
26	1264.0
27	1539.0
28	1435.0
29	1380.0
30	1568.0
31	1895.0
32	2527.0
33	3647.0
34	11628.0
35	52301.0
36	9533.0
37	14772.0
38	29512.0
39	159872.0
40	1161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.525359601651225	21.689941963772963	24.088750160461046	22.69594827411476
2	26.942592104640873	24.325894697015762	25.9426664234415	22.788846774901867
3	26.034213673308066	24.923147603894304	26.106843410287073	22.935795312510557
4	25.806527893197128	27.122646289802788	23.430690962158216	23.64013485484187
5	23.54048009945207	35.18319584355217	21.01566775442366	20.260656302572105
6	84.411631567924	2.5572423671213627	9.145941855672888	3.8851842092817424
7	83.81606772469614	2.377525994689584	7.208248035618975	6.598158244995305
8	71.61562316314328	4.736810101951882	15.059353696684704	8.588213038220132
9	44.837545858078116	18.49254447304592	18.75941652985251	17.91049313902345
10	37.54653370357609	18.460452263683106	28.6424657626798	15.35054827006101
11	34.60722513867213	16.927458094330827	27.375668024673843	21.089648742323206
12	19.49314577970556	29.4076115964354	31.86621264635737	19.233029977501673
13	14.239820013377383	32.83303268000351	32.077007789961556	20.850139516657546
14	12.49569288769078	22.732432048969333	44.90544621683524	19.866428846504654
15	14.266845031788177	18.283438393092403	45.1895467228787	22.260169852240715
16	15.048543689320388	22.152407591327673	30.19268838126896	32.60636033808298
17	17.6304463857416	22.63142604265899	35.34264345217586	24.395484119423553
18	21.604813155778963	28.14047604569931	29.350183432312466	20.90452736620927
19	23.340494963212194	22.962482518191216	27.63477038868733	26.062252129909265
20	18.822925323117875	31.18450655694509	27.730033578585378	22.262534541351656
21	18.78880623737425	24.16509583747154	26.87063799312213	30.175459932032076
22	16.135287242164434	29.771773719520844	25.081919587057715	29.011019451257003
23	16.423779313699658	22.774996452966334	36.787468498962916	24.013755734371095
24	19.203640269979935	22.417590584483584	33.13436163528387	25.244407510252614
25	15.36102046469519	28.753268338164055	30.40415915033342	25.48155204680733
26	17.859145604042943	25.93354547972786	27.964813426029146	28.242495490200053
27	20.577186830708527	24.84207254866192	34.12617981096	20.45456080966955
28	17.818945889156886	23.802622778036767	29.291404017268984	29.087027315537355
29	18.698610238428227	25.921384221443	26.752741350305044	28.62726418982373
30	26.894960509691845	24.85626068332759	22.320638330934862	25.928140476045698
31	25.338995074690395	24.92956604576687	21.42543459607732	28.30600428346542
32	23.356709974258667	25.296430670693393	25.324131314564458	26.02272804048348
33	18.549634824438723	25.875779502874785	25.488646114140167	30.085939558546325
34	19.369506320476184	24.119153306173192	29.67448365324199	26.836856720108642
35	22.82229023518522	26.287911033639393	26.82739796366486	24.06240076751052
36	19.515441419894465	29.68630709879671	29.472809453351438	21.32544202795738
37	18.31249028788401	33.72418266209944	26.56897122511165	21.394355824904906
38	16.48965279607597	30.80075129551182	27.575315348183583	25.134280560228632
39	22.55778286748958	25.643364344541958	29.229584287654298	22.569268500314166
40	15.833620474153948	21.948368702326178	33.164089155535734	29.053921667984135
41	20.89641986068603	24.947132307733884	25.23934031930059	28.917107512279493
42	21.929451189438623	20.225185965907936	30.25045435812203	27.594908486531406
43	22.903365290417604	24.339069393491027	28.510043172466908	24.24752214362446
44	16.810574889704142	28.40701028977576	29.506928539095068	25.27548628142503
45	21.935194005850917	27.11352534608914	25.86159136820912	25.089689279850823
46	22.40745620257954	22.52974441088838	28.848869340792238	26.213930045739843
47	19.968448291005398	20.567052448804482	32.852288005621205	26.61221125456892
48	20.10154650667856	18.73745870239374	29.492402591699268	31.668592199228435
49	20.29714007742668	22.063562843302186	32.23206383309349	25.407233246177647
50	19.385383518792523	23.75566680854801	30.137962718987104	26.72098695367236
51	18.561796082723582	19.90189918316882	32.302666693691684	29.233638040415915
52	19.773868158447684	18.848261277877995	36.412158555783016	24.965712007891305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	84.5
2	128.0
3	759.5
4	1391.0
5	943.0
6	495.0
7	546.5
8	598.0
9	586.5
10	575.0
11	569.0
12	563.0
13	560.0
14	507.0
15	457.0
16	429.5
17	402.0
18	451.0
19	500.0
20	496.5
21	493.0
22	459.0
23	425.0
24	477.0
25	529.0
26	605.5
27	682.0
28	1089.0
29	1496.0
30	1624.0
31	1752.0
32	2127.5
33	2503.0
34	2914.0
35	3325.0
36	3655.0
37	3985.0
38	4650.0
39	6544.0
40	7773.0
41	11724.5
42	15676.0
43	22536.0
44	29396.0
45	36736.5
46	44077.0
47	43937.5
48	43798.0
49	38151.0
50	32504.0
51	27963.5
52	23423.0
53	19962.5
54	16502.0
55	14523.5
56	12545.0
57	11456.5
58	10368.0
59	9701.0
60	9034.0
61	8335.0
62	7636.0
63	6895.0
64	5070.5
65	3987.0
66	3258.5
67	2530.0
68	2117.0
69	1704.0
70	1406.0
71	1108.0
72	907.5
73	707.0
74	547.0
75	387.0
76	322.0
77	257.0
78	191.0
79	125.0
80	99.0
81	73.0
82	53.0
83	33.0
84	22.5
85	12.0
86	6.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	296022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.25125132842109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27319505878613	24.06944865609643
2	10.812172731988387	7.40660891299271
3	3.7414657398858955	3.8444965018052635
4	1.9725106962055496	2.702438384149406
5	1.4736270060528012	2.5236784474331597
6	1.1427104883008736	2.3483558478249607
7	1.075727446295528	2.579150778676411
8	1.0037456835299619	2.750363654112203
9	0.9407616416099776	2.900003708422921
>10	7.3771308750592155	40.100452743160574
>50	0.1339660573597949	3.0130042071530134
>100	0.046988094745599694	3.2937933252193248
>500	0.0029992400901446615	0.8125763136236052
>1k	0.0029992400901446615	1.655628519330017
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1916	0.6472491909385114	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	1634	0.5519860010404631	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1285	0.43408935822337524	No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTG	972	0.32835397369114455	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.2540351730614616	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	649	0.21924046185756463	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	493	0.16654167595651673	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	441	0.14897541398950076	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	438	0.147961975799096	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	436	0.14728635033882617	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	411	0.13884103208545312	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	393	0.13276040294302452	No Hit
CTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTGAAA	346	0.11688320462668315	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5648228847855903	0.0
2	0.0	0.0	0.0	1.9738397821783515	0.0
3	0.0	0.0	0.0	3.0460573876265955	0.0
4	0.0	0.0	0.0	3.7507347426880435	0.0
5	0.0	0.0	0.0	7.337968123990785	0.0
6	0.0	0.0	0.0	8.070346122923297	0.0
7	0.0	0.0	0.0	11.613663849308498	0.0
8	0.0	0.0	0.0	15.276905094891596	0.0
9	0.0	0.0	0.0	19.03372046672207	0.0
10	0.0	0.0	0.0	20.53664930309234	0.0
11	0.0	0.0	0.0	22.05072595955706	0.0
12	0.0	0.0	0.0	23.344210903243678	0.0
13	0.0	0.0	0.0	23.768841505023275	0.0
14	3.378127301349224E-4	0.0	0.0	24.116112991601977	0.0
15	6.756254602698448E-4	0.0	0.0	25.351494145705388	0.0
16	6.756254602698448E-4	0.0	0.0	26.60477937450595	0.0
17	6.756254602698448E-4	0.0	0.0	28.33235367641594	0.0
18	6.756254602698448E-4	0.0	0.0	29.071825742681288	0.0
19	6.756254602698448E-4	0.0	0.0	30.065670794738228	0.0
20	6.756254602698448E-4	0.0	0.0	31.100391187141497	0.0
21	6.756254602698448E-4	0.0	0.0	32.00978305666471	0.0
22	6.756254602698448E-4	0.0	0.0	32.9722115248191	0.0
23	6.756254602698448E-4	0.0	0.0	33.642769794136925	0.0
24	6.756254602698448E-4	0.0	0.0	34.225834566349796	0.0
25	6.756254602698448E-4	0.0	0.0	34.78525244745323	0.0
26	6.756254602698448E-4	0.0	0.0	35.290620291735074	0.0
27	6.756254602698448E-4	0.0	0.0	35.82605346899893	0.0
28	6.756254602698448E-4	0.0	0.0	36.31182817493294	0.0
29	6.756254602698448E-4	0.0	0.0	36.971576436886444	0.0
30	6.756254602698448E-4	0.0	0.0	37.60227280404835	0.0
31	6.756254602698448E-4	0.0	0.0	38.08703407179196	0.0
32	6.756254602698448E-4	0.0	0.0	38.60929255258055	0.0
33	6.756254602698448E-4	0.0	0.0	39.109255393180234	0.0
34	6.756254602698448E-4	0.0	0.0	39.755491145928346	0.0
35	6.756254602698448E-4	0.0	0.0	40.26119680294032	0.0
36	6.756254602698448E-4	0.0	0.0	40.75676807804825	0.0
37	6.756254602698448E-4	0.0	0.0	41.237813405760384	0.0
38	6.756254602698448E-4	0.0	0.0	41.68372620953848	0.0
39	6.756254602698448E-4	0.0	0.0	42.23469877238854	0.0
40	6.756254602698448E-4	0.0	0.0	42.75695725317713	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCG	35	1.01612386E-7	46.000004	4
ACTAAGG	35	1.01612386E-7	46.000004	1
GCGATAG	35	1.01612386E-7	46.000004	8
TGCTAGG	35	1.01612386E-7	46.000004	1
ACAACGG	20	6.3038245E-4	46.0	1
CTATGTT	20	6.3038245E-4	46.0	10
TTGGCGT	20	6.3038245E-4	46.0	23
CCTTACT	20	6.3038245E-4	46.0	46
TCGTTCA	25	3.4109675E-5	46.0	15
CTCGTTC	25	3.4109675E-5	46.0	14
TCCGCCG	20	6.3038245E-4	46.0	15
TCCGCAA	20	6.3038245E-4	46.0	31
CACGGGT	30	1.856688E-6	46.0	3
GGATGCC	25	3.4109675E-5	46.0	7
TAGACGG	60	0.0	46.0	1
CCCTAGG	20	6.3038245E-4	46.0	1
AACCCGG	20	6.3038245E-4	46.0	1
GGCGTTT	20	6.3038245E-4	46.0	36
CAGTTGG	25	3.4109675E-5	46.0	1
CTATAGG	25	3.4109675E-5	46.0	1
>>END_MODULE