FastQCFastQC Report
Thu 26 May 2016
SRR1544956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544956_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1046076
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT37040.3540851716318891TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT31300.29921344147079176TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT27150.2595413717550159TruSeq Adapter, Index 20 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.1782853253492098No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17330.16566673931913167No Hit
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC12390.11844263705505145TruSeq Adapter, Index 20 (95% over 23bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11740.1122289393887251No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11640.11127298590159797No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT11190.10697119520952589TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC206.311191E-446.0000047
CGGCATA206.311191E-446.0000047
TCGATTG206.311191E-446.00000432
CGACACC405.6097633E-946.00000435
CGTTAGA206.311191E-446.00000426
AATGCGG405.6097633E-946.0000041
CATAGCG206.311191E-446.00000415
GCGATTC351.0194526E-746.0000048
TCGTACC206.311191E-446.00000429
TCATCGG206.311191E-446.0000041
ACCGTCA206.311191E-446.00000442
TAATGCG206.311191E-446.00000426
ATACCGG206.311191E-446.0000041
TACACGG405.6097633E-946.0000041
TACCGGT206.311191E-446.00000440
TACCGAA206.311191E-446.0000048
GAATCGG253.416944E-546.042
TAAACGG453.092282E-1046.01
TTACTCG301.8612427E-646.038
TAACCGG600.046.01
Produced by FastQC (version 0.11.5)