FastQCFastQC Report
Thu 26 May 2016
SRR1544955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544955_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences599443
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT27350.45625689181456786TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT20530.34248460654307417TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT20190.3368126744327651TruSeq Adapter, Index 20 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18420.3072852631526267No Hit
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTTG12740.212530632603934TruSeq Adapter, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.20235451911190888No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9260.1544767392395941No Hit
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC7720.1287862232105471TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT7090.11827646665320973TruSeq Adapter, Index 23 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC6960.11610778672867979TruSeq Adapter, Index 23 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6660.11110314074899531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG700.046.0000041
CGGTCTA351.01847036E-746.00000430
CGGGTCA405.6043064E-946.05
GTACCTA253.415186E-546.08
ACGTTAC206.3090253E-446.01
ATTCGCG206.3090253E-446.09
ATTCGAT206.3090253E-446.013
CTAACGG453.092282E-1046.01
GCTACGA551.8189894E-1246.010
ATTGCGG900.046.01
CATCCAG206.3090253E-446.037
TACGGGA1100.043.9090923
ACGGGAT1050.043.8095254
CGTTTTT10050.043.024881
GTCCCCC2950.042.8813559
TCGGCGT2800.042.7142874
ATCGCAT650.042.4615410
GTACGGG650.042.461542
ATAGCGG1200.042.1666681
GTTACGG601.8189894E-1242.1666681
Produced by FastQC (version 0.11.5)