##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544955_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	599443
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91429877402856	34.0	33.0	34.0	31.0	34.0
2	33.27360566392468	34.0	34.0	34.0	31.0	34.0
3	33.392139035738175	34.0	34.0	34.0	31.0	34.0
4	36.61555644156325	37.0	37.0	37.0	35.0	37.0
5	36.62063282080198	37.0	37.0	37.0	35.0	37.0
6	36.42463920673025	37.0	37.0	37.0	35.0	37.0
7	36.240444879663286	37.0	37.0	37.0	35.0	37.0
8	36.37797588761567	37.0	37.0	37.0	35.0	37.0
9	38.24071179411554	39.0	39.0	39.0	37.0	39.0
10	38.13721237882501	39.0	39.0	39.0	35.0	39.0
11	37.540715297367726	39.0	35.0	39.0	35.0	39.0
12	37.50302197206407	39.0	35.0	39.0	35.0	39.0
13	37.49075725298319	39.0	35.0	39.0	35.0	39.0
14	38.701676389581664	41.0	35.0	41.0	35.0	41.0
15	38.75130412733154	41.0	35.0	41.0	35.0	41.0
16	38.78388270444396	41.0	35.0	41.0	35.0	41.0
17	38.860210228495454	41.0	36.0	41.0	35.0	41.0
18	38.87010274538196	41.0	36.0	41.0	35.0	41.0
19	38.81866332578744	41.0	36.0	41.0	35.0	41.0
20	38.74944406724242	41.0	35.0	41.0	35.0	41.0
21	38.69503188793597	41.0	35.0	41.0	35.0	41.0
22	38.679233888793426	41.0	35.0	41.0	35.0	41.0
23	38.63663434221436	41.0	35.0	41.0	35.0	41.0
24	38.60335845109543	41.0	35.0	41.0	35.0	41.0
25	38.540992221111935	40.0	35.0	41.0	35.0	41.0
26	38.469609287288364	40.0	35.0	41.0	35.0	41.0
27	38.54593180669388	40.0	35.0	41.0	35.0	41.0
28	38.57009090105314	40.0	36.0	41.0	35.0	41.0
29	38.589934322362595	40.0	36.0	41.0	35.0	41.0
30	38.52814863131274	41.0	35.0	41.0	35.0	41.0
31	38.46257275504093	40.0	35.0	41.0	35.0	41.0
32	38.40038335588204	40.0	35.0	41.0	35.0	41.0
33	38.31883098142776	40.0	35.0	41.0	35.0	41.0
34	38.228660606596456	40.0	35.0	41.0	35.0	41.0
35	38.18294817021802	40.0	35.0	41.0	35.0	41.0
36	38.13541237448765	40.0	35.0	41.0	35.0	41.0
37	38.09204378064303	40.0	35.0	41.0	35.0	41.0
38	38.03258191354307	40.0	35.0	41.0	35.0	41.0
39	37.964690554398	40.0	35.0	41.0	35.0	41.0
40	37.89708946471975	40.0	35.0	41.0	35.0	41.0
41	37.86519485589122	40.0	35.0	41.0	35.0	41.0
42	37.79346159684908	40.0	35.0	41.0	35.0	41.0
43	37.7239620781292	40.0	35.0	41.0	35.0	41.0
44	37.57771464509553	40.0	35.0	41.0	35.0	41.0
45	37.55878373756971	39.0	35.0	41.0	35.0	41.0
46	37.47613534564587	39.0	35.0	41.0	35.0	41.0
47	37.372782733304085	39.0	35.0	41.0	35.0	41.0
48	37.298323610418336	39.0	35.0	41.0	35.0	41.0
49	37.18561064187921	39.0	35.0	41.0	35.0	41.0
50	36.980081509000854	38.0	35.0	40.0	35.0	41.0
51	36.81025718875689	37.0	35.0	40.0	34.0	41.0
52	36.322305873952985	37.0	35.0	39.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	1.0
15	6.0
16	19.0
17	27.0
18	43.0
19	78.0
20	141.0
21	216.0
22	341.0
23	597.0
24	910.0
25	1541.0
26	2398.0
27	2806.0
28	2704.0
29	2889.0
30	3333.0
31	4052.0
32	5398.0
33	7808.0
34	27904.0
35	115459.0
36	20762.0
37	33137.0
38	66266.0
39	299436.0
40	1166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37264760786263	21.684296922309542	23.18635800234551	21.756697467482315
2	27.960623445431843	23.394384453567728	25.93440910979026	22.710582991210174
3	27.578935778714573	24.318408922950137	25.287308384617052	22.815346913718233
4	26.47657909092274	26.727979140635554	22.475698273230314	24.319743495211387
5	24.614016678816835	36.027612300085245	20.390429115028454	18.96794190606947
6	86.12144941220433	2.9143721755029253	7.673957323715516	3.290221088577229
7	85.54908473366109	2.89685591457403	6.330710342768203	5.223349008996685
8	73.73711929240979	5.704128666111707	12.69028081068592	7.8684712307925855
9	49.05587353593253	18.758747704118655	17.003785180575967	15.181593579372851
10	40.72764216113959	20.42696303068015	24.88193206026261	13.963462747917651
11	38.749972891500946	17.253016550364254	24.332922396291224	19.66408816184358
12	22.700907342316118	29.774807613067466	28.285091326448054	19.239193718168366
13	16.261095717190795	32.43878066805351	29.778978151383868	21.52114546337183
14	15.104522031285711	23.35701643025275	41.776782780014116	19.761678758447427
15	17.207140628883813	17.862916073755137	43.68071693221874	21.249226365142306
16	17.834890056268904	21.927355895389553	28.200846452456695	32.03690759588484
17	19.932003543289355	23.509658132633128	32.067602757893575	24.49073556618394
18	24.930643947798206	27.120510206975474	27.09081597416268	20.85802987106364
19	27.486516649623066	23.213383090635805	25.108642523142315	24.19145773659881
20	22.16274107796738	29.609153831139906	26.645736125036073	21.582368965856638
21	20.77695460619275	24.637538514921353	25.336353915217963	29.24915296366794
22	19.906313027260307	28.60221906002739	24.134404772430408	27.357063140281895
23	19.44655288325996	22.117365621084907	35.480104029907764	22.955977465747367
24	21.300273754135088	22.112527796637877	31.822875569487007	24.764322879740025
25	18.589924313070632	28.882479234889725	28.628076397589098	23.899520054450548
26	19.961030489971524	27.900567693675626	27.120176563910164	25.018225252442683
27	21.996920474507167	26.169961113900737	32.72321138123224	19.109907030359853
28	20.315859889931154	25.179541674521182	28.580031796184123	25.924566639363544
29	23.819779361840908	25.823973255171882	25.96243512727649	24.393812255710717
30	28.239882691098234	24.496073855228936	21.792564097003382	25.47147935666944
31	29.390951266425663	26.101230642446403	22.397792617479894	22.110025473648037
32	27.33470905490597	24.84172807089248	23.634440639059928	24.18912223514162
33	22.122370267064593	25.440116908530086	25.73455691366819	26.702955910737135
34	21.48210922473029	23.970752848894726	29.530914532324175	25.01622339405081
35	26.067199049784552	23.71835186998597	25.411590426445883	24.802858653783595
36	21.96005291579016	28.54683431118555	24.875759663554334	24.61735310946996
37	21.040866270854778	26.919990724722787	28.407204688352355	23.631938316070087
38	19.912318602435928	27.227442809408064	27.08931458036877	25.770924007787226
39	25.5762432791775	22.762130844800925	28.39052253508674	23.271103340934836
40	18.170034515375104	21.506298346965433	30.7904171038781	29.53325003378136
41	22.527579769886376	25.558727018248607	24.91396179453259	26.999731417332423
42	22.017773166089185	22.30353845152917	27.468166281030893	28.210522101350755
43	23.2605935843775	26.51077750511725	26.38516089102717	23.84346801947808
44	19.41836004424107	26.332445286707827	27.7614385354404	26.487756133610702
45	22.279015686228714	25.54438036644018	26.595022379108606	25.5815815682225
46	23.548193906676698	22.408135552504575	28.42989241679359	25.61377812402514
47	21.562517203470556	20.338881261437702	31.9271390273971	26.171462507694642
48	20.653840315092513	19.90097473821531	28.93686305453563	30.50832189215655
49	20.190243275841073	23.028378010920136	30.9817614018347	25.799617311404088
50	19.522456680618507	24.33358968242185	28.654434199748767	27.48951943721088
51	19.96686924361449	21.027020083644317	30.446931568139092	28.559179104602105
52	21.845780165920697	19.303420008240984	33.74382551802256	25.106974307815754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	209.0
2	369.0
3	1427.5
4	2486.0
5	1708.5
6	931.0
7	903.5
8	876.0
9	914.0
10	952.0
11	912.5
12	873.0
13	801.5
14	745.5
15	761.0
16	736.5
17	712.0
18	759.0
19	806.0
20	843.5
21	881.0
22	929.5
23	978.0
24	1196.5
25	1415.0
26	1739.5
27	2064.0
28	2712.0
29	3360.0
30	3691.5
31	4023.0
32	5023.0
33	6023.0
34	6884.0
35	7745.0
36	8283.5
37	8822.0
38	10121.5
39	13118.0
40	14815.0
41	20777.5
42	26740.0
43	35378.5
44	44017.0
45	49550.0
46	55083.0
47	61501.5
48	67920.0
49	69233.5
50	70547.0
51	63767.5
52	56988.0
53	50264.5
54	43541.0
55	38499.5
56	33458.0
57	30034.5
58	26611.0
59	26452.5
60	26294.0
61	24772.0
62	23250.0
63	21888.5
64	16127.5
65	11728.0
66	9624.0
67	7520.0
68	6330.5
69	5141.0
70	4287.0
71	3433.0
72	2758.5
73	2084.0
74	1844.0
75	1604.0
76	1383.5
77	1163.0
78	778.5
79	394.0
80	309.5
81	225.0
82	121.5
83	18.0
84	16.0
85	14.0
86	14.0
87	14.0
88	16.5
89	11.5
90	4.0
91	8.5
92	13.0
93	6.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	599443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.926709708521113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.938037168577	19.810659712991306
2	11.153980782075818	6.229879667909093
3	3.9380064530300034	3.2992668913215533
4	1.9930119698097288	2.2263306690593656
5	1.0933318625269823	1.5266580769933873
6	0.8138707180261261	1.3637238769548758
7	0.6485700801524481	1.2678699847834782
8	0.529882866920002	1.1838308003195055
9	0.49677749641226104	1.2486024838827992
>10	8.029153374353655	49.75797717019421
>50	0.2795825752086256	5.205605227844963
>100	0.07679521379121046	4.002765100042362
>500	0.005399663469694485	1.0109955886837845
>1k	0.0035997756464629903	1.8658347490193277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2735	0.45625689181456786	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2053	0.34248460654307417	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	2019	0.3368126744327651	TruSeq Adapter, Index 20 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1842	0.3072852631526267	No Hit
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTTG	1274	0.212530632603934	TruSeq Adapter, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1213	0.20235451911190888	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	926	0.1544767392395941	No Hit
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	772	0.1287862232105471	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	709	0.11827646665320973	TruSeq Adapter, Index 23 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	696	0.11610778672867979	TruSeq Adapter, Index 23 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	666	0.11110314074899531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.343485535739011	0.0
2	0.0	0.0	0.0	1.3706057123029212	0.0
3	0.0	0.0	0.0	2.185028434730241	0.0
4	0.0	0.0	0.0	2.664807162649326	0.0
5	0.0	0.0	0.0	5.399679369014235	0.0
6	0.0	0.0	0.0	5.901478539243931	0.0
7	0.0	0.0	0.0	8.289695600749363	0.0
8	0.0	0.0	0.0	10.787848052275196	0.0
9	0.0	0.0	0.0	13.120346721873473	0.0
10	0.0	0.0	0.0	14.231044486298114	0.0
11	0.0	0.0	0.0	15.301538261352622	0.0
12	0.0	0.0	0.0	16.25392239128658	0.0
13	0.0	0.0	0.0	16.636110522601815	0.0
14	0.0	0.0	0.0	16.934554244523667	0.0
15	0.0	0.0	0.0	17.94515908935462	0.0
16	0.0	0.0	0.0	18.934243956472926	0.0
17	0.0	0.0	0.0	20.267648466993524	0.0
18	0.0	0.0	0.0	20.89606518050924	0.0
19	0.0	0.0	0.0	21.6274107796738	0.0
20	0.0	0.0	0.0	22.442834431297054	0.0
21	0.0	0.0	0.0	23.189193968400666	0.0
22	0.0	0.0	0.0	24.009121801405637	0.0
23	0.0	0.0	0.0	24.606676531379964	0.0
24	0.0	0.0	0.0	25.12232188882012	0.0
25	0.0	0.0	0.0	25.596929149226867	0.0
26	0.0	0.0	0.0	26.048848681192375	0.0
27	0.0	0.0	0.0	26.50393782227835	0.0
28	0.0	0.0	0.0	26.953188209721358	0.0
29	0.0	0.0	0.0	27.500362836833528	0.0
30	0.0	0.0	0.0	28.049872965402884	0.0
31	0.0	0.0	0.0	28.539994628346648	0.0
32	0.0	0.0	0.0	29.02077428546167	0.0
33	0.0	0.0	0.0	29.470525137502648	0.0
34	0.0	0.0	0.0	30.071082655064785	0.0
35	0.0	0.0	0.0	30.54652402313481	0.0
36	0.0	0.0	0.0	31.050158230223726	0.0
37	0.0	0.0	0.0	31.541781286961395	0.0
38	0.0	0.0	0.0	32.011050258323145	0.0
39	0.0	0.0	0.0	32.51902182526112	0.0
40	0.0	0.0	0.0	33.04050593634424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGG	70	0.0	46.000004	1
CGGTCTA	35	1.01847036E-7	46.000004	30
CGGGTCA	40	5.6043064E-9	46.0	5
GTACCTA	25	3.415186E-5	46.0	8
ACGTTAC	20	6.3090253E-4	46.0	1
ATTCGCG	20	6.3090253E-4	46.0	9
ATTCGAT	20	6.3090253E-4	46.0	13
CTAACGG	45	3.092282E-10	46.0	1
GCTACGA	55	1.8189894E-12	46.0	10
ATTGCGG	90	0.0	46.0	1
CATCCAG	20	6.3090253E-4	46.0	37
TACGGGA	110	0.0	43.909092	3
ACGGGAT	105	0.0	43.809525	4
CGTTTTT	1005	0.0	43.02488	1
GTCCCCC	295	0.0	42.881355	9
TCGGCGT	280	0.0	42.714287	4
ATCGCAT	65	0.0	42.46154	10
GTACGGG	65	0.0	42.46154	2
ATAGCGG	120	0.0	42.166668	1
GTTACGG	60	1.8189894E-12	42.166668	1
>>END_MODULE