FastQCFastQC Report
Thu 26 May 2016
SRR1544954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544954_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences492046
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT20290.4123598200168277RNA PCR Primer, Index 38 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT15830.3217178881649277RNA PCR Primer, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT14730.2993622547485398RNA PCR Primer, Index 38 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC7920.16096056059799285RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT7650.15547326875942494RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.14023079143006956No Hit
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC5150.10466501099490697RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGG351.0179792E-746.0000041
TCACCGG351.0179792E-746.0000041
TACTTGG351.0179792E-746.0000041
TTTACGG351.0179792E-746.0000041
CCGGCAG351.0179792E-746.00000428
GCCCCCG206.307919E-446.014
GGTACTC206.307919E-446.07
CGGCGAA206.307919E-446.021
CTCACGG453.092282E-1046.01
ACCGGGT405.6006684E-946.03
ACACGCT206.307919E-446.02
ACACGAC206.307919E-446.010
TCACCGC206.307919E-446.020
ATAGGTG405.6006684E-946.033
GTCGAGG551.8189894E-1246.01
TTTACCC453.092282E-1046.022
TGAAGCG206.307919E-446.012
GCGGTAT206.307919E-446.04
TTAGCGG650.046.01
TGGACAA206.307919E-446.021
Produced by FastQC (version 0.11.5)