FastQCFastQC Report
Thu 26 May 2016
SRR1544953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544953_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences296247
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT17910.6045630841831309RNA PCR Primer, Index 38 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT12100.4084429546965877RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT11000.3713117769968978RNA PCR Primer, Index 27 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTTG7100.23966487424345226RNA PCR Primer, Index 27 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC5330.17991743376304234RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT4930.1664151873267915RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.15190027240782186No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4280.14447403686788388No Hit
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC3910.13198445891435187RNA PCR Primer, Index 27 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC3250.109705752294538RNA PCR Primer, Index 38 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT3180.10734285916819411RNA PCR Primer, Index 27 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC3020.10194196059369377RNA PCR Primer, Index 38 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATGG351.01614205E-746.0000041
CTGTAGG351.01614205E-746.0000041
CATGCGG850.046.0000041
TAGACGG700.046.0000041
TACGGGC351.01614205E-746.0000043
TAGCAGG700.046.0000041
AACGGGA351.01614205E-746.0000043
CACTAGG351.01614205E-746.0000041
ATTGGGC351.01614205E-746.0000043
AGGGCGA850.046.0000045
ATATTGG351.01614205E-746.0000041
ATGGGTA206.3038315E-446.04
GCCCCAC206.3038315E-446.032
TGTCAGG501.6370905E-1146.01
CGGGTCC253.410974E-546.05
GGGTGAT405.5879354E-946.06
CTGTCGG206.3038315E-446.01
TACCCCG253.410974E-546.024
GCGACGG453.092282E-1046.01
ATTAGGC206.3038315E-446.026
Produced by FastQC (version 0.11.5)