FastQCFastQC Report
Thu 26 May 2016
SRR1544952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544952_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences655455
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT28510.4349650242960997No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT23060.35181667696485647No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT22180.3383908887719218Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT9970.15210807759495312No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.14219130222517182No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC9120.13913998672677758No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC7470.11396663386502505No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC7220.11015248949203225No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT6940.1058806477942803No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC6610.1008459772219298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG351.01867045E-746.0000048
GTTACGG700.046.0000041
ATATCAC351.01867045E-746.00000429
GAACGAA351.01867045E-746.0000048
ACTCATG351.01867045E-746.00000421
GTTGATA253.4155375E-546.012
AGGTATA206.309459E-446.05
CGGGTGT405.6043064E-946.05
CATGGCC301.8601695E-646.041
CGGGTAC405.6043064E-946.05
ATCTCGG405.6043064E-946.01
ACACGTC253.4155375E-546.023
TCCGCTA206.309459E-446.039
ACGCCCG253.4155375E-546.014
TTAATCG405.6043064E-946.019
CGAGTAG206.309459E-446.022
CACGGGA1200.046.03
CGCGGCT206.309459E-446.045
ACGTTAT253.4155375E-546.032
CCTAGGT206.309459E-446.02