##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544952_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	655455
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91781281705075	34.0	33.0	34.0	31.0	34.0
2	33.19913190074071	34.0	33.0	34.0	31.0	34.0
3	33.23539831109687	34.0	33.0	34.0	31.0	34.0
4	36.53249879854452	37.0	37.0	37.0	35.0	37.0
5	36.520800054923676	37.0	37.0	37.0	35.0	37.0
6	36.6468331159271	37.0	37.0	37.0	35.0	37.0
7	36.481673036287766	37.0	37.0	37.0	35.0	37.0
8	36.516850127011004	37.0	37.0	37.0	35.0	37.0
9	38.3099282178029	39.0	39.0	39.0	37.0	39.0
10	38.07580993355761	39.0	39.0	39.0	35.0	39.0
11	37.059506754849686	39.0	35.0	39.0	35.0	39.0
12	36.883091897994525	39.0	35.0	39.0	35.0	39.0
13	36.853100517960804	39.0	35.0	39.0	35.0	39.0
14	38.023209831338534	40.0	35.0	41.0	35.0	41.0
15	38.12613375441487	40.0	35.0	41.0	35.0	41.0
16	38.16646299135715	40.0	35.0	41.0	35.0	41.0
17	38.286791618036325	40.0	36.0	41.0	35.0	41.0
18	38.26817706783837	40.0	36.0	41.0	35.0	41.0
19	38.14662333798659	40.0	35.0	41.0	35.0	41.0
20	38.03778443981662	40.0	35.0	41.0	35.0	41.0
21	37.980282399249376	40.0	35.0	41.0	35.0	41.0
22	37.95773012640075	40.0	35.0	41.0	35.0	41.0
23	37.95315925578415	40.0	35.0	41.0	35.0	41.0
24	37.913080226712744	40.0	35.0	41.0	35.0	41.0
25	37.86660716601445	40.0	35.0	41.0	35.0	41.0
26	37.82529082850844	40.0	35.0	41.0	35.0	41.0
27	37.81282773035525	40.0	35.0	41.0	35.0	41.0
28	37.86176320266075	40.0	35.0	41.0	35.0	41.0
29	37.88341838875285	40.0	35.0	41.0	35.0	41.0
30	37.81197183635795	40.0	35.0	41.0	35.0	41.0
31	37.74119962468819	40.0	35.0	41.0	35.0	41.0
32	37.67276166937471	40.0	35.0	41.0	35.0	41.0
33	37.60017239932566	39.0	35.0	41.0	35.0	41.0
34	37.550204056723956	39.0	35.0	41.0	35.0	41.0
35	37.46047402186267	39.0	35.0	41.0	35.0	41.0
36	37.4178593496121	39.0	35.0	41.0	35.0	41.0
37	37.32724290759854	39.0	35.0	41.0	34.0	41.0
38	37.31832543805448	39.0	35.0	41.0	35.0	41.0
39	37.17590376150918	39.0	35.0	41.0	34.0	41.0
40	37.169225957540945	38.0	35.0	41.0	34.0	41.0
41	37.15697797713039	38.0	35.0	41.0	34.0	41.0
42	37.0512148049828	38.0	35.0	41.0	34.0	41.0
43	36.989176983927194	38.0	35.0	41.0	34.0	41.0
44	36.916551098092164	38.0	35.0	41.0	34.0	41.0
45	36.89629951712932	38.0	35.0	41.0	34.0	41.0
46	36.933572861599956	38.0	35.0	41.0	34.0	41.0
47	36.91444416474052	38.0	35.0	41.0	34.0	41.0
48	36.97145799482802	37.0	35.0	41.0	34.0	41.0
49	36.884669428107195	37.0	35.0	41.0	34.0	41.0
50	36.81738944702535	37.0	35.0	41.0	34.0	41.0
51	36.75769045929926	37.0	35.0	41.0	34.0	41.0
52	36.584163672563335	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	10.0
15	12.0
16	13.0
17	20.0
18	61.0
19	89.0
20	215.0
21	320.0
22	504.0
23	814.0
24	1400.0
25	2183.0
26	3136.0
27	3534.0
28	3608.0
29	3964.0
30	4713.0
31	5834.0
32	7815.0
33	11470.0
34	36488.0
35	184812.0
36	20527.0
37	28250.0
38	51044.0
39	281924.0
40	2686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.029437566270758	22.686378164786294	26.004073506190355	20.280110762752592
2	26.085391064222563	24.022701787308055	27.753392681419776	22.138514467049607
3	25.04824892631836	25.914669962087405	27.03663867084697	22.000442440747268
4	25.087305764697803	27.277997726769954	23.899581206947847	23.735115301584393
5	22.360650235332706	36.08821353105858	21.408182102508945	20.14295413109977
6	87.08988412629395	2.4585974628311633	7.656971111670519	2.7945472992043694
7	86.76888573586288	2.1476684135447894	5.454073887604793	5.629371962987543
8	76.74027965306543	4.157875063886919	12.165900023647696	6.935945259399959
9	55.296702290775116	15.955786438428268	14.378561457308281	14.36894981348834
10	48.767192255761266	16.526229870853072	23.68980326643324	11.016774606952422
11	45.009649785263676	15.149171186427749	22.774866314239727	17.066312714068854
12	19.011831475845025	39.29712947494489	27.14267188441617	14.548367164793921
13	12.44936723344852	43.14758450236859	27.61608348399204	16.78696478019086
14	10.649548786720676	20.426116209350756	53.16947769106957	15.754857312859006
15	11.506968441769457	16.02886544461481	53.19541387280592	19.26875224080982
16	10.98137934717105	19.121068570687537	28.883752507799926	41.01379957434148
17	16.830751157592815	21.894714358727907	37.882539609889314	23.39199487378996
18	23.848471672349742	26.649884431425498	27.832269187053267	21.66937470917149
19	27.573670198564354	21.534811695692305	25.38206284184269	25.509455263900648
20	16.835175565065487	33.59071179562289	27.38586172963819	22.18825090967343
21	19.451068341838877	24.18442150872295	23.912091600491262	32.452418548946916
22	15.24986459787476	33.28069814098603	21.153092126843184	30.316345134296025
23	15.390682808125652	22.19008169897247	40.30299562899055	22.11623986391133
24	14.901556933733056	22.249582351191158	37.988115126133756	24.86074558894203
25	12.398410264625337	32.12989450076664	31.34967312782723	24.122022106780786
26	16.598088350840257	31.05949302392994	27.0430464333936	25.299372191836206
27	15.791778230389578	27.641867099953465	40.052482626572385	16.513872043084575
28	14.726716555675065	22.973964650509952	35.18151513071073	27.117803663104254
29	16.525467041978473	28.58563898360681	29.62339138461069	25.26550258980403
30	26.949523613367816	23.872424499012137	24.111037370986566	25.067014516633485
31	28.288440854063207	22.504367195307076	25.44644559885881	23.760746351770905
32	22.76250848647123	24.001037447269454	27.664294268866662	25.57215979739265
33	14.87699384397098	24.918415451861684	32.10807759495312	28.09651310921421
34	14.518921970234416	20.496296465813824	37.40928057608836	27.575500987863393
35	26.55071667772769	20.491414361016393	30.707981478514924	22.249887482740995
36	18.174550503085644	26.587942726808095	32.752210296664146	22.485296473442112
37	17.026798178364647	29.171491559298502	26.20576546063422	27.595944801702633
38	15.830835068769023	26.4277486631424	29.50789909299647	28.23351717509211
39	23.254533110587303	20.429167524849152	28.270895789947442	28.045403574616106
40	13.302209915249714	18.36571541906004	33.58323607265182	34.74883859303842
41	20.9442295809781	21.10244028956984	26.070744749830272	31.88258537962179
42	20.135936105453464	19.833703305337515	31.964055503428916	28.066305085780108
43	21.745199899306588	26.184253686370536	30.506289524071068	21.56425689025181
44	13.32951918896034	33.002418167532475	28.225126057471527	25.442936586035653
45	20.74116453455996	28.713336537214605	27.01116018643538	23.534338741790055
46	21.989762836502887	20.432829103447222	29.466706333768144	28.110701726281746
47	21.67517220861844	17.5488782601399	32.65288997719142	28.12305955405024
48	19.699445423408164	15.98614702763729	32.25621896240016	32.05818858655437
49	19.740180485311733	24.302507418510803	31.166136500598824	24.791175595578643
50	17.89520256920765	25.903685226293188	27.742560511400477	28.458551693098688
51	17.941277433233402	19.479293010199022	34.18480292316025	28.394626633407327
52	21.825449496914356	16.95295634330351	35.61770068120618	25.60389347857595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	151.0
2	176.0
3	920.5
4	1665.0
5	1173.0
6	681.0
7	813.5
8	946.0
9	1049.5
10	1153.0
11	1108.5
12	1064.0
13	1072.5
14	1037.5
15	994.0
16	959.5
17	925.0
18	913.0
19	901.0
20	930.0
21	959.0
22	1038.5
23	1118.0
24	1035.0
25	952.0
26	1232.0
27	1512.0
28	1982.5
29	2453.0
30	2838.0
31	3223.0
32	3650.5
33	4078.0
34	5576.5
35	7075.0
36	7987.5
37	8900.0
38	10079.5
39	14326.0
40	17393.0
41	31119.5
42	44846.0
43	63355.5
44	81865.0
45	94446.0
46	107027.0
47	103613.5
48	100200.0
49	85698.0
50	71196.0
51	59702.0
52	48208.0
53	41432.5
54	34657.0
55	28518.0
56	22379.0
57	20246.0
58	18113.0
59	17218.0
60	16323.0
61	15217.5
62	14112.0
63	12011.5
64	8435.0
65	6959.0
66	5412.5
67	3866.0
68	3229.0
69	2592.0
70	2166.0
71	1740.0
72	1333.0
73	926.0
74	874.0
75	822.0
76	751.5
77	681.0
78	478.0
79	275.0
80	188.5
81	102.0
82	57.0
83	12.0
84	8.0
85	4.0
86	3.0
87	2.0
88	2.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	655455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.143597574255978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.6228923787386	15.195577132162649
2	12.741472590403436	5.64284083090613
3	5.2038914851371585	3.4569863660092355
4	2.7102478369564267	2.400585497122434
5	1.4623990577574293	1.6191388113975815
6	0.9351991713521999	1.2425204461320463
7	0.6440178020820005	0.9982609727972458
8	0.4636061535192337	0.8212726477182918
9	0.36531478787440297	0.728044528555392
>10	4.565256623513701	26.63355081783688
>50	1.9736702929961931	28.805153983335924
>100	0.30443812494274963	10.38275725689049
>500	0.005522687073791377	0.9459298583006485
>1k	0.002071007652671766	1.127380850835049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2851	0.4349650242960997	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2306	0.35181667696485647	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	2218	0.3383908887719218	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	997	0.15210807759495312	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	932	0.14219130222517182	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	912	0.13913998672677758	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	747	0.11396663386502505	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	722	0.11015248949203225	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	694	0.1058806477942803	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	661	0.1008459772219298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3451037828683891	0.0
2	0.0	0.0	0.0	1.411996246881937	0.0
3	0.0	0.0	0.0	2.2834519532233335	0.0
4	0.0	0.0	0.0	2.810719271345859	0.0
5	0.0	0.0	0.0	5.836861416878351	0.0
6	0.0	0.0	0.0	6.474433790267829	0.0
7	0.0	0.0	0.0	9.962087404932452	0.0
8	0.0	0.0	0.0	13.35072583167418	0.0
9	0.0	0.0	0.0	17.00437100945145	0.0
10	0.0	0.0	0.0	18.211166289066373	0.0
11	0.0	0.0	0.0	19.453204262687752	0.0
12	0.0	0.0	0.0	20.531996857145035	0.0
13	1.5256577491971226E-4	0.0	0.0	20.85421577377547	0.0
14	1.5256577491971226E-4	0.0	0.0	21.115255814663097	0.0
15	1.5256577491971226E-4	0.0	0.0	22.113341114187854	0.0
16	1.5256577491971226E-4	0.0	0.0	23.130344569802656	0.0
17	1.5256577491971226E-4	0.0	0.0	24.982035380003204	0.0
18	1.5256577491971226E-4	0.0	0.0	25.566972561045382	0.0
19	1.5256577491971226E-4	0.0	0.0	26.361382551052323	0.0
20	1.5256577491971226E-4	0.0	0.0	27.173337605175032	0.0
21	1.5256577491971226E-4	0.0	0.0	27.793212348673823	0.0
22	1.5256577491971226E-4	0.0	0.0	28.617830362114866	0.0
23	1.5256577491971226E-4	0.0	0.0	29.15120031123418	0.0
24	1.5256577491971226E-4	0.0	0.0	29.566789482115478	0.0
25	1.5256577491971226E-4	0.0	0.0	30.055915356508073	0.0
26	1.5256577491971226E-4	0.0	0.0	30.426650189562974	0.0
27	1.5256577491971226E-4	0.0	0.0	30.834000808598606	0.0
28	1.5256577491971226E-4	0.0	0.0	31.204735641653507	0.0
29	1.5256577491971226E-4	0.0	0.0	31.844596501666782	0.0
30	1.5256577491971226E-4	0.0	0.0	32.41198861859319	0.0
31	1.5256577491971226E-4	0.0	0.0	32.83963048569314	0.0
32	1.5256577491971226E-4	0.0	0.0	33.231877093011725	0.0
33	1.5256577491971226E-4	0.0	0.0	33.65250093446537	0.0
34	1.5256577491971226E-4	0.0	0.0	34.28854765010565	0.0
35	1.5256577491971226E-4	0.0	0.0	34.70947662310914	0.0
36	1.5256577491971226E-4	0.0	0.0	35.128269675263745	0.0
37	1.5256577491971226E-4	0.0	0.0	35.51975345370773	0.0
38	1.5256577491971226E-4	0.0	0.0	35.88270743224172	0.0
39	1.5256577491971226E-4	0.0	0.0	36.317825022312746	0.0
40	1.5256577491971226E-4	0.0	0.0	36.725480772898216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAACG	35	1.01867045E-7	46.000004	8
GTTACGG	70	0.0	46.000004	1
ATATCAC	35	1.01867045E-7	46.000004	29
GAACGAA	35	1.01867045E-7	46.000004	8
ACTCATG	35	1.01867045E-7	46.000004	21
GTTGATA	25	3.4155375E-5	46.0	12
AGGTATA	20	6.309459E-4	46.0	5
CGGGTGT	40	5.6043064E-9	46.0	5
CATGGCC	30	1.8601695E-6	46.0	41
CGGGTAC	40	5.6043064E-9	46.0	5
ATCTCGG	40	5.6043064E-9	46.0	1
ACACGTC	25	3.4155375E-5	46.0	23
TCCGCTA	20	6.309459E-4	46.0	39
ACGCCCG	25	3.4155375E-5	46.0	14
TTAATCG	40	5.6043064E-9	46.0	19
CGAGTAG	20	6.309459E-4	46.0	22
CACGGGA	120	0.0	46.0	3
CGCGGCT	20	6.309459E-4	46.0	45
ACGTTAT	25	3.4155375E-5	46.0	32
CCTAGGT	20	6.309459E-4	46.0	2
>>END_MODULE