FastQCFastQC Report
Thu 26 May 2016
SRR1544951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544951_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences335306
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT17910.5341389656015699No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT14060.41931847327515764Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT12590.3754779216596184No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTG8320.24813155744305201Illumina Single End Adapter 1 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG5740.1711869158321056No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.16402927475201756No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT4880.14553870196179014No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC4690.13987223610672045No Hit
CTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTGAAA3870.11541696241641963Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC3820.11392578719140128No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC3790.11303108205639029No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC3620.10796108629132792No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT3580.10676814611131324No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC206.3050294E-446.034
CGGAATT206.3050294E-446.025
ATGGGCG206.3050294E-446.04
CGGCGTC501.6370905E-1146.05
TCGTTCC253.411944E-546.039
GTCGCAT206.3050294E-446.041
TCGCAAG206.3050294E-446.042
TCCGCGG301.8574319E-646.01
ACGCCAA206.3050294E-446.043
GTACAGG405.5915734E-946.01
GAATGAC206.3050294E-446.08
ACGTTAT206.3050294E-446.032
CCTAGGC206.3050294E-446.02
GCGCGAA206.3050294E-446.017
TTAGACT206.3050294E-446.013
CGTTGGA206.3050294E-446.027
GAGTAGT301.8574319E-646.013
GGCAACG206.3050294E-446.07
TCTTACC206.3050294E-446.044
TTCACGG206.3050294E-446.01
Produced by FastQC (version 0.11.5)