FastQCFastQC Report
Thu 26 May 2016
SRR1544950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544950_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2132508
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT56760.2661654727672768No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT55460.26006936433532724No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.2181468955802276No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT44930.21069088603653538Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38500.18053859586927692No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25410.11915547327372278No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA22800.10691636326803933No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22030.1033055913506538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATT206.312676E-446.012
CGGTCTA1350.044.296330
CGAATAT3500.041.414
CTAGCGG5500.041.41
CAACGCA16950.041.25073616
CGTTTTT32200.041.1428571
CGCACTT14900.041.06040234
ATCAACG17300.040.41618714
AACGCAA17450.040.3323817
ACCCGCA15300.040.28758231
CATGCGG3150.040.158731
CTAACGG2350.040.127661
CCGCACT15250.040.1180333
TCAACGC17700.039.6327715
ACATACG6400.039.5312516
CGTAGGG6700.039.477612
TGTACGC355.3752756E-639.42857445
AAGCTTA17400.039.39080422
CCCGCAC15650.039.3865832
CACTTAC15650.039.23961636