##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544950_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2132508
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89166980850717	34.0	33.0	34.0	31.0	34.0
2	33.22267536628233	34.0	33.0	34.0	31.0	34.0
3	33.29553183387823	34.0	34.0	34.0	31.0	34.0
4	36.57293618593693	37.0	37.0	37.0	35.0	37.0
5	36.568372545378494	37.0	37.0	37.0	35.0	37.0
6	36.63429961341294	37.0	37.0	37.0	35.0	37.0
7	36.43261314846181	37.0	37.0	37.0	35.0	37.0
8	36.47096751805855	37.0	37.0	37.0	35.0	37.0
9	38.298143781875616	39.0	39.0	39.0	37.0	39.0
10	38.16323596441373	39.0	39.0	39.0	35.0	39.0
11	37.740445053430044	39.0	37.0	39.0	35.0	39.0
12	37.699761501480886	39.0	37.0	39.0	35.0	39.0
13	37.68747127795066	39.0	37.0	39.0	35.0	39.0
14	39.08298726194697	41.0	38.0	41.0	35.0	41.0
15	39.12322486011776	41.0	38.0	41.0	35.0	41.0
16	39.11825371815721	41.0	38.0	41.0	35.0	41.0
17	39.136188469164004	41.0	38.0	41.0	35.0	41.0
18	39.122016892785396	41.0	38.0	41.0	35.0	41.0
19	39.07562363189259	41.0	38.0	41.0	35.0	41.0
20	38.99108561374682	41.0	38.0	41.0	35.0	41.0
21	38.943210529573626	41.0	37.0	41.0	35.0	41.0
22	38.91150279389339	41.0	37.0	41.0	35.0	41.0
23	38.88659128125193	41.0	37.0	41.0	35.0	41.0
24	38.81473926475305	41.0	37.0	41.0	35.0	41.0
25	38.772292999604225	41.0	36.0	41.0	35.0	41.0
26	38.704761717189335	41.0	36.0	41.0	35.0	41.0
27	38.657784167749895	41.0	36.0	41.0	35.0	41.0
28	38.69726491061229	41.0	36.0	41.0	35.0	41.0
29	38.696235137218714	41.0	36.0	41.0	35.0	41.0
30	38.643278243270366	41.0	36.0	41.0	35.0	41.0
31	38.58808407752749	41.0	36.0	41.0	35.0	41.0
32	38.51548880473133	41.0	36.0	41.0	35.0	41.0
33	38.41788776407873	40.0	36.0	41.0	35.0	41.0
34	38.327543437117235	40.0	36.0	41.0	35.0	41.0
35	38.25163516385402	40.0	35.0	41.0	35.0	41.0
36	38.1998013606514	40.0	35.0	41.0	35.0	41.0
37	38.12120892395245	40.0	35.0	41.0	35.0	41.0
38	38.03812459320199	40.0	35.0	41.0	35.0	41.0
39	37.93118431443165	40.0	35.0	41.0	35.0	41.0
40	37.82086116441298	40.0	35.0	41.0	35.0	41.0
41	37.75885764555162	40.0	35.0	41.0	35.0	41.0
42	37.68768604854003	40.0	35.0	41.0	35.0	41.0
43	37.6006364337203	40.0	35.0	41.0	34.0	41.0
44	37.531281008090005	40.0	35.0	41.0	34.0	41.0
45	37.467237168629616	39.0	35.0	41.0	34.0	41.0
46	37.47034946644983	39.0	35.0	41.0	34.0	41.0
47	37.403046553635434	39.0	35.0	41.0	34.0	41.0
48	37.379833979520825	39.0	35.0	41.0	34.0	41.0
49	37.32370617132503	39.0	35.0	41.0	34.0	41.0
50	37.249947010749786	39.0	35.0	41.0	34.0	41.0
51	37.16624837984195	39.0	35.0	41.0	34.0	41.0
52	36.941424369803066	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	9.0
13	15.0
14	14.0
15	40.0
16	57.0
17	95.0
18	229.0
19	375.0
20	614.0
21	998.0
22	1545.0
23	2370.0
24	3810.0
25	6249.0
26	8670.0
27	10486.0
28	11331.0
29	12352.0
30	14341.0
31	17413.0
32	22274.0
33	31095.0
34	92351.0
35	299648.0
36	87396.0
37	144002.0
38	245318.0
39	1109332.0
40	10077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.90190142311307	21.351619782903512	22.639352349440188	19.107126444543233
2	29.77916143808136	23.625796480013207	24.31395333569675	22.281088746208688
3	29.41648987952214	24.09158605735594	23.623826968058268	22.868097095063654
4	26.747566714872818	26.46986552922662	21.12878357314486	25.6537841827557
5	24.99212195218025	36.862182932021824	19.785951565011715	18.359743550786213
6	86.36338058286299	3.8220255211234844	7.070641704509432	2.7439521915040883
7	85.4910743593928	4.066573255528232	5.9950537114046	4.447298673674378
8	73.45895068154492	7.130242887717185	11.812429308588762	7.598377122149132
9	46.76784330938032	22.03761955406498	15.868873645491599	15.325663491063105
10	35.022705659251926	23.29876370921	24.941993183612908	16.736537447925166
11	34.208406252168814	18.653622870347967	26.448294684005873	20.689676193477354
12	23.30908020040253	26.372890512016838	29.315294479551778	21.002734808028855
13	17.91627510893277	28.98568258595044	30.617517026899783	22.48052527821701
14	17.128282754390607	24.71817221787679	37.25186494024876	20.901680087483847
15	19.66810909970795	19.768413529984414	39.04210441414522	21.521372956162416
16	20.43973574776742	22.54007956828298	28.124490037083095	28.895694646866506
17	21.508618021597105	23.43475382038426	29.937050646468855	25.119577511549778
18	24.189358257976053	26.767074261855058	27.206556786656837	21.837010693512052
19	27.066486972147352	22.676585504016867	26.06367713509164	24.193250388744143
20	23.997471521794996	27.146955603449086	27.01124685112553	21.84432602363039
21	22.18678663808061	23.840989107661027	26.1113674602862	27.860856793972168
22	21.911008071247565	26.853216963312683	24.92337660632457	26.31239835911518
23	21.809906457560768	22.128170210850325	32.81005276416313	23.251870567425772
24	22.380502206791252	22.237384338065787	29.46488360184346	25.917229853299496
25	20.09605591163081	26.550146587961216	28.372226505129174	24.981570995278798
26	20.39439007966207	26.61490601676524	27.552440600457302	25.438263303115395
27	22.478133727986013	25.9297034290141	30.49029593323917	21.101866909760712
28	20.701118120072703	25.644686913249565	27.123602818840535	26.530592147837197
29	21.680856531370573	24.85125495426043	27.195536898337547	26.272351616031454
30	24.60394052449041	26.157463418660093	24.164082854554355	25.07451320229514
31	25.604030559322638	24.320049444128696	24.08595888034183	25.989961116206832
32	23.498809852061516	25.468134234431943	25.389025504241953	25.644030409264584
33	19.83992557120536	25.03629529174099	27.074974630810296	28.048804506243354
34	21.549461948091167	23.665749436813364	27.030801291249553	27.753987323845912
35	24.043286121318186	24.006146753025075	25.84595227778747	26.104614847869268
36	23.441084394525134	26.043559977266206	26.394367570954014	24.12098805725465
37	21.810516068403967	25.788789537952496	26.998820168552705	25.401874225090832
38	22.322636069829514	23.85726102786015	25.52510940169978	28.29499350061055
39	25.377489791363033	21.82055120074579	27.798301342832012	25.00365766505917
40	19.704685750299646	21.58599170554108	30.375126377017107	28.33419616714216
41	21.53937992260756	24.97603760454826	24.051867566264697	29.432714906579484
42	22.384769482693617	22.289717084296985	27.88383443344644	27.441678999562956
43	22.955599697633023	24.975427993705065	27.497341158860834	24.571631149801078
44	20.47560900123235	25.729516606737228	27.91164206652449	25.88323232550593
45	21.632978633608875	25.04839372232132	26.190054152200133	27.128573491869666
46	23.074567598339605	22.87438077606274	27.510096093426146	26.540955532171505
47	22.197431381265627	21.138068415218136	30.56480913553431	26.099691067981922
48	21.38031838567546	21.33042408281704	28.048945185668707	29.2403123458388
49	20.607613195354954	23.566570441939724	30.278807863792306	25.547008498913016
50	20.59016894661122	23.771399685253233	28.193047810371635	27.445383557763908
51	20.47912598686617	22.375015709202497	29.136678502495656	28.00917980143568
52	22.202308268011187	20.367473416277925	31.389096781817464	26.041121533893424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	822.0
2	1424.0
3	5235.0
4	9046.0
5	6054.5
6	3063.0
7	3071.5
8	3080.0
9	3062.0
10	3044.0
11	2850.5
12	2657.0
13	2689.5
14	2676.5
15	2631.0
16	2778.0
17	2925.0
18	2876.0
19	2827.0
20	3526.0
21	4225.0
22	4414.5
23	4604.0
24	5408.5
25	6213.0
26	8119.5
27	10026.0
28	13873.5
29	17721.0
30	19751.5
31	21782.0
32	25497.5
33	29213.0
34	31459.0
35	33705.0
36	37291.5
37	40878.0
38	45069.5
39	59302.0
40	69343.0
41	79575.5
42	89808.0
43	108306.5
44	126805.0
45	136394.5
46	145984.0
47	153346.5
48	160709.0
49	178973.0
50	197237.0
51	205032.0
52	212827.0
53	193947.5
54	175068.0
55	155757.0
56	136446.0
57	122702.5
58	108959.0
59	104915.0
60	100871.0
61	98837.5
62	96804.0
63	90102.0
64	69849.5
65	56299.0
66	48151.0
67	40003.0
68	34039.5
69	28076.0
70	23342.0
71	18608.0
72	16009.5
73	13411.0
74	11706.0
75	10001.0
76	7487.5
77	4974.0
78	3828.0
79	2682.0
80	2105.5
81	1529.0
82	1257.0
83	985.0
84	654.5
85	324.0
86	199.0
87	74.0
88	40.5
89	5.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2132508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.757394054829188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5632833218538	14.647098777142755
2	11.726385277828886	4.868184001012837
3	4.438109400678804	2.7637075706499528
4	2.2247010244700105	1.8471598327642482
5	1.2244846877258386	1.2708555588614852
6	0.8009401470046393	0.9975258147424871
7	0.5499190996729709	0.7990421215132123
8	0.42504643101819667	0.7058285008194785
9	0.3311217009520497	0.6185901264090304
>10	5.301432570522994	29.170654254212113
>50	2.1517944534752176	29.581390545861158
>100	0.24503558158460487	8.108184825763228
>500	0.010693285268742383	1.5544615505417212
>1k	0.006597984527521896	2.537340292448885
>5k	4.550334156911652E-4	0.529976227257457
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	5676	0.2661654727672768	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	5546	0.26006936433532724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4652	0.2181468955802276	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	4493	0.21069088603653538	Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3850	0.18053859586927692	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2541	0.11915547327372278	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2280	0.10691636326803933	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2203	0.1033055913506538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.689314178422777E-5	0.0	0.0	0.2173028190281115	0.0
2	4.689314178422777E-5	0.0	0.0	0.9069602552487493	0.0
3	4.689314178422777E-5	0.0	0.0	1.4412607127382406	0.0
4	4.689314178422777E-5	0.0	0.0	1.7984457737087036	0.0
5	4.689314178422777E-5	0.0	0.0	3.820665620011742	0.0
6	4.689314178422777E-5	0.0	0.0	4.206596176895936	0.0
7	4.689314178422777E-5	0.0	0.0	5.804667555760635	0.0
8	4.689314178422777E-5	0.0	0.0	7.538494580090672	0.0
9	4.689314178422777E-5	0.0	0.0	9.062709260645212	0.0
10	9.378628356845554E-5	0.0	0.0	9.914898326289983	0.0
11	1.4067942535268333E-4	0.0	0.0	10.717943379344884	0.0
12	1.8757256713691108E-4	0.0	0.0	11.381668908158844	0.0
13	1.8757256713691108E-4	0.0	0.0	11.675454441437031	0.0
14	1.8757256713691108E-4	0.0	0.0	11.886708045174977	0.0
15	2.3446570892113886E-4	0.0	0.0	12.631558709275652	0.0
16	2.3446570892113886E-4	0.0	0.0	13.405248655573626	0.0
17	2.3446570892113886E-4	0.0	0.0	14.36585466502353	0.0
18	2.3446570892113886E-4	0.0	0.0	14.855137706400164	0.0
19	3.282519924895944E-4	0.0	0.0	15.431641991495459	0.0
20	3.7514513427382216E-4	0.0	0.0	16.100197513913194	0.0
21	4.2203827605804997E-4	0.0	0.0	16.711355830787035	0.0
22	4.2203827605804997E-4	0.0	0.0	17.359325263961495	0.0
23	4.2203827605804997E-4	0.0	0.0	17.862019743888418	0.0
24	4.2203827605804997E-4	0.0	0.0	18.312897302143767	0.0
25	4.2203827605804997E-4	0.0	0.0	18.72452530072572	0.0
26	4.2203827605804997E-4	0.0	0.0	19.125883701256924	0.0
27	4.2203827605804997E-4	0.0	0.0	19.52902404117593	0.0
28	4.2203827605804997E-4	0.0	0.0	19.9346497176095	0.0
29	4.2203827605804997E-4	0.0	0.0	20.413194229517543	0.0
30	4.2203827605804997E-4	0.0	0.0	20.909558135303595	0.0
31	4.2203827605804997E-4	0.0	0.0	21.361092197543925	0.0
32	4.2203827605804997E-4	0.0	0.0	21.795463369891227	0.0
33	4.2203827605804997E-4	0.0	0.0	22.207044475331394	0.0
34	4.2203827605804997E-4	0.0	0.0	22.70669090104234	0.0
35	4.2203827605804997E-4	0.0	0.0	23.132386842159562	0.0
36	4.2203827605804997E-4	0.0	0.0	23.581998285586735	0.0
37	4.2203827605804997E-4	0.0	0.0	24.01125810547956	0.0
38	4.2203827605804997E-4	0.0	0.0	24.430623472455906	0.0
39	4.2203827605804997E-4	0.0	0.0	24.902649837655943	0.0
40	4.2203827605804997E-4	0.0	0.0	25.36023311518644	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCATT	20	6.312676E-4	46.0	12
CGGTCTA	135	0.0	44.2963	30
CGAATAT	350	0.0	41.4	14
CTAGCGG	550	0.0	41.4	1
CAACGCA	1695	0.0	41.250736	16
CGTTTTT	3220	0.0	41.142857	1
CGCACTT	1490	0.0	41.060402	34
ATCAACG	1730	0.0	40.416187	14
AACGCAA	1745	0.0	40.33238	17
ACCCGCA	1530	0.0	40.287582	31
CATGCGG	315	0.0	40.15873	1
CTAACGG	235	0.0	40.12766	1
CCGCACT	1525	0.0	40.11803	33
TCAACGC	1770	0.0	39.63277	15
ACATACG	640	0.0	39.53125	16
CGTAGGG	670	0.0	39.47761	2
TGTACGC	35	5.3752756E-6	39.428574	45
AAGCTTA	1740	0.0	39.390804	22
CCCGCAC	1565	0.0	39.38658	32
CACTTAC	1565	0.0	39.239616	36
>>END_MODULE