FastQCFastQC Report
Thu 26 May 2016
SRR1544949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544949_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1289893
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA54800.42484144033652405No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT50240.3894896708486673No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT39050.3027382891449136No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT37580.29134199503369657Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.2387794956635938No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTTG23280.18048008633274232Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22950.17792173459348953No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13770.10675304075609372No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13400.10388458577571939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA206.3117425E-446.013
CGGTATG206.3117425E-446.05
ACGTAGG1200.044.0833321
CGCACTT9400.043.0638334
TAGGGCG1500.042.933334
ATTGCGG2200.042.863641
ATAGCGG2000.042.551
CATGCGG1950.042.461541
TCAACGC10600.042.09433715
TACGCGG1750.042.0571441
CAACGCA10650.041.89671316
ATCAACG10600.041.87735414
CGTTTTT21600.041.6342621
CGGTAGT1050.041.61904511
CCGTAGG508.712959E-1041.3999981
CCCGCAC9800.041.3061232
ACCCGCA9750.041.04615431
TCGGCGT8750.041.0057144
TACGGGC1800.040.888893
CCGCACT9900.040.88888533