##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544949_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1289893
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84922160210188	34.0	33.0	34.0	31.0	34.0
2	33.2392787618818	34.0	33.0	34.0	31.0	34.0
3	33.37627539648638	34.0	34.0	34.0	31.0	34.0
4	36.5923902215145	37.0	37.0	37.0	35.0	37.0
5	36.606521626212405	37.0	37.0	37.0	35.0	37.0
6	36.37335965076173	37.0	37.0	37.0	35.0	37.0
7	36.148061118247796	37.0	37.0	37.0	35.0	37.0
8	36.30379651645524	37.0	37.0	37.0	35.0	37.0
9	38.22027253423346	39.0	39.0	39.0	37.0	39.0
10	38.163634503016915	39.0	39.0	39.0	35.0	39.0
11	37.78544576953282	39.0	37.0	39.0	35.0	39.0
12	37.7948690317724	39.0	37.0	39.0	35.0	39.0
13	37.78854137513732	39.0	38.0	39.0	35.0	39.0
14	39.10034320676211	41.0	38.0	41.0	35.0	41.0
15	39.1161538205107	41.0	38.0	41.0	35.0	41.0
16	39.13398320635898	41.0	38.0	41.0	35.0	41.0
17	39.184064879800104	41.0	38.0	41.0	35.0	41.0
18	39.195409231618434	41.0	38.0	41.0	35.0	41.0
19	39.18399975811947	41.0	38.0	41.0	35.0	41.0
20	39.11613676483243	41.0	38.0	41.0	35.0	41.0
21	39.061531460361444	41.0	38.0	41.0	35.0	41.0
22	39.03706586515315	41.0	38.0	41.0	35.0	41.0
23	38.99640125188679	41.0	38.0	41.0	35.0	41.0
24	38.93495119362614	41.0	37.0	41.0	35.0	41.0
25	38.89121578301456	41.0	37.0	41.0	35.0	41.0
26	38.800485776727214	41.0	37.0	41.0	35.0	41.0
27	38.86496476839552	41.0	37.0	41.0	35.0	41.0
28	38.87974041257686	41.0	37.0	41.0	35.0	41.0
29	38.88858300649744	41.0	37.0	41.0	35.0	41.0
30	38.84678186485235	41.0	37.0	41.0	35.0	41.0
31	38.80388605876612	41.0	37.0	41.0	35.0	41.0
32	38.72705100345532	41.0	37.0	41.0	35.0	41.0
33	38.652889813341105	41.0	36.0	41.0	35.0	41.0
34	38.540870444292665	41.0	36.0	41.0	35.0	41.0
35	38.46811867340934	41.0	36.0	41.0	35.0	41.0
36	38.431619521929335	40.0	36.0	41.0	35.0	41.0
37	38.358571602450745	40.0	35.0	41.0	35.0	41.0
38	38.263887004581	40.0	35.0	41.0	35.0	41.0
39	38.2120168106967	40.0	35.0	41.0	35.0	41.0
40	38.13024336127105	40.0	35.0	41.0	35.0	41.0
41	38.071340801136216	40.0	35.0	41.0	35.0	41.0
42	38.01225993163774	40.0	35.0	41.0	35.0	41.0
43	37.95163087170796	40.0	35.0	41.0	35.0	41.0
44	37.816986370187294	40.0	35.0	41.0	35.0	41.0
45	37.794839571964495	40.0	35.0	41.0	35.0	41.0
46	37.723349146014435	40.0	35.0	41.0	35.0	41.0
47	37.622540784390644	40.0	35.0	41.0	35.0	41.0
48	37.547621391851884	39.0	35.0	41.0	35.0	41.0
49	37.5024486527177	39.0	35.0	41.0	35.0	41.0
50	37.43272736575825	39.0	35.0	41.0	35.0	41.0
51	37.33778848323078	39.0	35.0	41.0	34.0	41.0
52	36.84999530968848	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	7.0
14	10.0
15	18.0
16	24.0
17	63.0
18	86.0
19	190.0
20	337.0
21	499.0
22	762.0
23	1122.0
24	2021.0
25	3351.0
26	4652.0
27	5829.0
28	5703.0
29	6037.0
30	6598.0
31	8100.0
32	11074.0
33	15748.0
34	53673.0
35	175056.0
36	52130.0
37	88193.0
38	154661.0
39	688612.0
40	5333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.84467858961945	21.042598106974765	21.99159155061699	21.121131752788795
2	29.7305280360464	23.903455557941626	24.24402644250337	22.1219899635086
3	29.62028633382769	23.932837840037894	23.626920992671486	22.81995483346293
4	26.717487419499136	26.028127914485932	21.740873080170214	25.513511585844718
5	24.924858108385735	36.07082137820734	19.982742754631584	19.02157775877534
6	85.22482097352261	3.7188355933399126	7.695754609103236	3.360588824034241
7	84.4675488587038	3.7946558357941322	6.606904603715192	5.1308907017868925
8	71.83967972537258	6.965383950451703	12.683765242543373	8.511171081632353
9	46.73418647903353	21.144699599114038	16.25755004484868	15.86356387700375
10	34.34982591579301	22.7698731600218	26.038593898873785	16.841707025311404
11	33.2560917843573	18.32516340502662	26.686864724438387	21.73188008617769
12	22.91244312512743	25.377221211371797	29.991790016691304	21.718545646809464
13	17.87559123121065	27.905182832994672	31.030790926069063	23.188435009725612
14	17.063895997574992	24.208829724636075	36.81119286638504	21.916081411403894
15	19.564335956548334	19.306640163176326	38.88477571395457	22.244248166320773
16	20.14105045922414	22.87437795227976	28.335140976809704	28.649430611686395
17	21.17795817172432	23.55148837926867	30.06272613309786	25.20782731590915
18	23.69398081856402	26.806176946459896	27.374673713245983	22.125168521730096
19	26.55972239557855	22.53551263554419	26.25419317726354	24.650571791613725
20	23.480242159620992	27.38661268802916	27.0658884108992	22.06725674145065
21	21.868558089702013	23.53195187507801	26.192172529039233	28.407317506180746
22	21.560548045458034	26.884478014843094	25.134565425194182	26.42040851450469
23	21.581324962613177	21.899723465434732	32.866292010267514	23.652659561684576
24	22.27153725153947	22.393407825300237	29.05310750581637	26.281947417343922
25	20.254005564802664	26.115111873620528	28.332039944398492	25.298842617178323
26	20.29959074124753	25.96068045954199	27.270479024229143	26.469249774981336
27	23.037104628058298	25.38768719575965	30.211730740456765	21.36347743572529
28	21.024999748041118	25.21100587413064	26.873934504644957	26.89005987318328
29	21.8018083670506	24.163709703052888	27.00410034010573	27.03038158979078
30	24.402644250337044	26.017894507528922	23.78949261682946	25.789968625304578
31	25.502502920784902	23.578699938677083	23.494894537763987	27.42390260277403
32	22.840731750618072	25.215657422747466	25.439319385406385	26.504291441228073
33	19.481848494410002	24.711584604304388	27.062554801057143	28.744012100228467
34	21.768859897681438	23.0659442294826	26.96169372188236	28.203502150953607
35	23.581103238795777	23.24999050308824	26.401027061934595	26.767879196181386
36	23.78034457121637	25.280236422711035	26.184032318959787	24.755386687112807
37	21.847083440254348	25.261010021761493	27.08185872781696	25.8100478101672
38	22.256729821775913	23.114707964148966	25.125882534442777	29.502679679632344
39	25.804543477637292	20.997090456340178	27.571744322978724	25.626621743043803
40	19.842653615454925	20.903904432383154	30.388412062085767	28.865029890076155
41	21.66156417625338	24.506916465164164	23.960747131738835	29.870772226843624
42	22.247116621301146	21.606598376764584	27.94123233477506	28.205052667159215
43	23.35216952103779	24.447299117058545	27.43258549352543	24.76794586837823
44	20.74311590186163	24.802522379763285	28.22226339704146	26.23209832133363
45	21.456430882251475	24.19991425645383	26.330556100389725	28.013098760904974
46	23.22223626300786	21.786768359856204	27.581667626694617	27.409327750441314
47	21.85119230819921	20.60543006280366	30.895430861319507	26.647946767677627
48	21.249281917182277	20.388667897259698	28.007982057426467	30.354068128131555
49	20.588762013593374	22.44356702455165	30.566643899920383	26.401027061934595
50	20.694894847867225	22.83398700512368	28.51965240527703	27.95146574173207
51	20.73815425000368	21.448678301223435	29.093420927162178	28.719746521610706
52	22.027330949156248	19.962508518148407	31.80000201567107	26.21015851702428
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	514.0
2	912.0
3	3369.0
4	5826.0
5	3916.5
6	2007.0
7	1955.5
8	1904.0
9	1888.0
10	1872.0
11	1800.0
12	1728.0
13	1710.0
14	1674.0
15	1656.0
16	1700.5
17	1745.0
18	1758.5
19	1772.0
20	2084.5
21	2397.0
22	2426.5
23	2456.0
24	2902.0
25	3348.0
26	4451.5
27	5555.0
28	7423.5
29	9292.0
30	10319.0
31	11346.0
32	13391.5
33	15437.0
34	16754.0
35	18071.0
36	20088.5
37	22106.0
38	24656.0
39	32175.0
40	37144.0
41	43333.5
42	49523.0
43	60510.5
44	71498.0
45	77246.5
46	82995.0
47	89142.0
48	95289.0
49	110700.5
50	126112.0
51	132594.0
52	139076.0
53	126392.5
54	113709.0
55	100012.0
56	86315.0
57	76603.5
58	66892.0
59	64093.5
60	61295.0
61	59489.5
62	57684.0
63	56231.5
64	44916.5
65	35054.0
66	29740.5
67	24427.0
68	20800.5
69	17174.0
70	14408.0
71	11642.0
72	9946.0
73	8250.0
74	7240.0
75	6230.0
76	4696.5
77	3163.0
78	2358.5
79	1554.0
80	1206.0
81	858.0
82	698.5
83	539.0
84	364.0
85	189.0
86	117.0
87	45.0
88	26.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1289893.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.381719794529232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54255361632383	18.158730492746155
2	10.872370835693935	5.519189401075902
3	3.81409827032847	2.9042512069882753
4	1.85862612287339	1.887005098142586
5	1.1057129459590298	1.4032448083757771
6	0.75884603847337	1.1556490509431776
7	0.5750571398861152	1.0217157433302944
8	0.5063350935848122	1.0281324374005152
9	0.41924346917087063	0.9577008234163255
>10	8.019709295653644	49.94930315741437
>50	0.40539904084692113	6.733222803034253
>100	0.1082487193869815	4.870070656529955
>500	0.009506261475133037	1.7358528971821048
>1k	0.0036798431516644014	1.8583635446650495
>5k	6.13307191944067E-4	0.8175678787553058
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5480	0.42484144033652405	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	5024	0.3894896708486673	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	3905	0.3027382891449136	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	3758	0.29134199503369657	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3080	0.2387794956635938	No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTTG	2328	0.18048008633274232	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2295	0.17792173459348953	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1377	0.10675304075609372	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1340	0.10388458577571939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2981642663383707	0.0
2	0.0	0.0	0.0	1.163274783257216	0.0
3	0.0	0.0	0.0	1.791931578820879	0.0
4	0.0	0.0	0.0	2.2196414741377772	0.0
5	0.0	0.0	0.0	4.538671037055011	0.0
6	0.0	0.0	0.0	4.984056817115838	0.0
7	7.75258102803876E-5	0.0	0.0	6.882353807641409	0.0
8	7.75258102803876E-5	0.0	0.0	8.90058322667074	0.0
9	7.75258102803876E-5	0.0	0.0	10.705229038377603	0.0
10	7.75258102803876E-5	0.0	0.0	11.70089301980862	0.0
11	1.550516205607752E-4	0.0	0.0	12.632830785189158	0.0
12	2.325774308411628E-4	0.0	0.0	13.425764772736963	0.0
13	2.325774308411628E-4	0.0	0.0	13.772460196310856	0.0
14	2.325774308411628E-4	0.0	0.0	14.012325053318376	0.0
15	2.325774308411628E-4	0.0	0.0	14.856038446599834	0.0
16	2.325774308411628E-4	0.0	0.0	15.704403388498116	0.0
17	3.101032411215504E-4	0.0	0.0	16.71185129309175	0.0
18	3.101032411215504E-4	0.0	0.0	17.255152171536707	0.0
19	3.101032411215504E-4	0.0	0.0	17.870397001921866	0.0
20	3.101032411215504E-4	0.0	0.0	18.603791167174332	0.0
21	3.101032411215504E-4	0.0	0.0	19.268497464518376	0.0
22	3.101032411215504E-4	0.0	0.0	19.96956336688392	0.0
23	3.101032411215504E-4	0.0	0.0	20.494645679912985	0.0
24	3.101032411215504E-4	0.0	0.0	20.955924251081292	0.0
25	3.101032411215504E-4	0.0	0.0	21.384719507742115	0.0
26	3.101032411215504E-4	0.0	0.0	21.83157827819827	0.0
27	3.87629051401938E-4	0.0	0.0	22.254636624898346	0.0
28	3.87629051401938E-4	0.0	0.0	22.676221981203092	0.0
29	3.87629051401938E-4	0.0	0.0	23.170371495930283	0.0
30	3.87629051401938E-4	0.0	0.0	23.6857630826743	0.0
31	3.87629051401938E-4	0.0	0.0	24.161073825503355	0.0
32	3.87629051401938E-4	0.0	0.0	24.61801095129596	0.0
33	4.651548616823256E-4	0.0	0.0	25.03579754289697	0.0
34	4.651548616823256E-4	0.0	0.0	25.54002541296061	0.0
35	4.651548616823256E-4	0.0	0.0	25.980759644404614	0.0
36	4.651548616823256E-4	0.0	0.0	26.439789967074788	0.0
37	4.651548616823256E-4	0.0	0.0	26.8777332693487	0.0
38	4.651548616823256E-4	0.0	0.0	27.317924820120737	0.0
39	4.651548616823256E-4	0.0	0.0	27.813392273622696	0.0
40	4.651548616823256E-4	0.0	0.0	28.283973942024648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAA	20	6.3117425E-4	46.0	13
CGGTATG	20	6.3117425E-4	46.0	5
ACGTAGG	120	0.0	44.083332	1
CGCACTT	940	0.0	43.06383	34
TAGGGCG	150	0.0	42.93333	4
ATTGCGG	220	0.0	42.86364	1
ATAGCGG	200	0.0	42.55	1
CATGCGG	195	0.0	42.46154	1
TCAACGC	1060	0.0	42.094337	15
TACGCGG	175	0.0	42.057144	1
CAACGCA	1065	0.0	41.896713	16
ATCAACG	1060	0.0	41.877354	14
CGTTTTT	2160	0.0	41.634262	1
CGGTAGT	105	0.0	41.619045	11
CCGTAGG	50	8.712959E-10	41.399998	1
CCCGCAC	980	0.0	41.30612	32
ACCCGCA	975	0.0	41.046154	31
TCGGCGT	875	0.0	41.005714	4
TACGGGC	180	0.0	40.88889	3
CCGCACT	990	0.0	40.888885	33
>>END_MODULE