FastQCFastQC Report
Thu 26 May 2016
SRR1544948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544948_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences757586
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT29330.38715076572164747No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT24790.3272235759372533Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT22910.30240791144503726No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.19298139089159513No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT13560.1789895800608776No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC10640.1404461011687122No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT10360.13675015113795663No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA8910.11761040990725805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA206.310084E-446.00000413
AACGTCC206.310084E-446.00000438
AATCCGG405.6061253E-946.0000041
CGAACGG206.310084E-446.00000418
TCCGCGA206.310084E-446.00000424
TCCGCCT206.310084E-446.00000446
GATCGTA206.310084E-446.0000048
ATAGGCG206.310084E-446.00000413
CCGATTC206.310084E-446.00000410
CTATCGG405.6061253E-946.0000041
GCGCGTC206.310084E-446.00000433
TTTAACG206.310084E-446.00000435
TTTCGAG206.310084E-446.00000413
ACTCTTA206.310084E-446.00000439
GGCCGGC206.310084E-446.0000048
TACGGGT405.6061253E-946.0000043
ATCACGG405.6061253E-946.0000041
CCGTCCG206.310084E-446.00000421
AACGGTC206.310084E-446.00000420
ATCCGGA206.310084E-446.0000049