##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544948_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	757586
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.938776587740534	34.0	33.0	34.0	31.0	34.0
2	33.24508900639663	34.0	33.0	34.0	31.0	34.0
3	33.30207131599581	34.0	34.0	34.0	31.0	34.0
4	36.58920307397444	37.0	37.0	37.0	35.0	37.0
5	36.577555551448945	37.0	37.0	37.0	35.0	37.0
6	36.67654893305843	37.0	37.0	37.0	35.0	37.0
7	36.52663591988237	37.0	37.0	37.0	35.0	37.0
8	36.567550086722825	37.0	37.0	37.0	35.0	37.0
9	38.362481619248506	39.0	39.0	39.0	37.0	39.0
10	38.147337727993914	39.0	39.0	39.0	35.0	39.0
11	37.34411934750642	39.0	35.0	39.0	35.0	39.0
12	37.245173485254476	39.0	35.0	39.0	35.0	39.0
13	37.23593493015974	39.0	35.0	39.0	35.0	39.0
14	38.51659613562025	41.0	35.0	41.0	35.0	41.0
15	38.58378454723292	41.0	35.0	41.0	35.0	41.0
16	38.59107348868643	41.0	35.0	41.0	35.0	41.0
17	38.682766049003014	41.0	36.0	41.0	35.0	41.0
18	38.66383618493478	40.0	36.0	41.0	35.0	41.0
19	38.566721137930216	40.0	35.0	41.0	35.0	41.0
20	38.47644227850039	40.0	35.0	41.0	35.0	41.0
21	38.44224022091221	40.0	35.0	41.0	35.0	41.0
22	38.41408764153508	40.0	35.0	41.0	35.0	41.0
23	38.401716768789285	40.0	35.0	41.0	35.0	41.0
24	38.35628958296484	40.0	35.0	41.0	35.0	41.0
25	38.33689244521414	40.0	35.0	41.0	35.0	41.0
26	38.28277845683527	40.0	35.0	41.0	35.0	41.0
27	38.27253935526792	40.0	35.0	41.0	35.0	41.0
28	38.27933462339589	40.0	35.0	41.0	35.0	41.0
29	38.31876117034898	40.0	36.0	41.0	35.0	41.0
30	38.214081305620745	40.0	35.0	41.0	35.0	41.0
31	38.14485220159823	40.0	35.0	41.0	35.0	41.0
32	38.0855295636403	40.0	35.0	41.0	35.0	41.0
33	38.004090624694754	40.0	35.0	41.0	35.0	41.0
34	37.88154084156782	40.0	35.0	41.0	35.0	41.0
35	37.84311220112304	40.0	35.0	41.0	35.0	41.0
36	37.784234397150954	40.0	35.0	41.0	35.0	41.0
37	37.7040138017334	40.0	35.0	41.0	35.0	41.0
38	37.69434625243867	40.0	35.0	41.0	35.0	41.0
39	37.57134899536158	40.0	35.0	41.0	35.0	41.0
40	37.52095339671008	40.0	35.0	41.0	34.0	41.0
41	37.48213140158345	39.0	35.0	41.0	35.0	41.0
42	37.39784130118561	39.0	35.0	41.0	35.0	41.0
43	37.31186690355946	39.0	35.0	41.0	34.0	41.0
44	37.25309865810614	39.0	35.0	41.0	34.0	41.0
45	37.23002933000346	39.0	35.0	41.0	34.0	41.0
46	37.23586365112344	39.0	35.0	41.0	34.0	41.0
47	37.20970556478076	39.0	35.0	41.0	34.0	41.0
48	37.23503734229513	39.0	35.0	41.0	35.0	41.0
49	37.15530118032804	39.0	35.0	41.0	34.0	41.0
50	37.08224280807723	39.0	35.0	41.0	34.0	41.0
51	37.017334005644244	38.0	35.0	41.0	34.0	41.0
52	36.82083618229481	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	11.0
15	6.0
16	9.0
17	25.0
18	58.0
19	103.0
20	179.0
21	302.0
22	438.0
23	791.0
24	1459.0
25	2495.0
26	3468.0
27	3948.0
28	3821.0
29	4026.0
30	4735.0
31	6059.0
32	7856.0
33	11706.0
34	36741.0
35	174799.0
36	23835.0
37	33381.0
38	65683.0
39	368252.0
40	3391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.570937160929585	22.65194974563944	25.419292331167682	20.357820762263295
2	25.63444942224381	24.34707082760241	27.49509626629848	22.523383483855298
3	25.35698917350637	24.94951068261557	27.40164152980652	22.29185861407154
4	25.461267763659833	28.0072493419889	22.71240492828537	23.819077966065898
5	22.286710683671558	36.35904042577344	20.926733070568886	20.427515819986112
6	88.12794851013615	2.743318910328332	6.861531232097742	2.2672013474377826
7	88.65528138059572	2.0194406971617744	4.846182479612875	4.479095442629616
8	79.39653055890685	4.2036151671229405	10.147890800516377	6.25196347345384
9	50.20644520886077	22.571562832470505	13.652971411826512	13.569020546842205
10	41.869041930553095	18.974875459683783	27.83525566734338	11.320826942419739
11	40.95693426224877	17.534378935196795	24.76141322569319	16.74727357686124
12	18.69015530910022	37.8396644077372	27.33458643639138	16.135593846771194
13	12.676580612630117	40.52503610151191	27.98203768285053	18.81634560300745
14	10.794418059467837	23.470206682805646	48.28890713397555	17.446468123750964
15	12.590253779768897	18.519613614823925	49.61020926997067	19.279923335436504
16	13.33379972702769	20.802654748107805	28.851509927585777	37.012035597278725
17	17.584406258827382	22.313242324963767	35.8720990092214	24.230252406987457
18	23.314448788652378	27.528227818359895	28.107303989250067	21.05001940373766
19	26.260648956026117	23.493834363359404	26.38512327313335	23.86039340748113
20	18.038480119748783	31.71177925674445	28.240490188572647	22.00925043493412
21	19.052358412114266	24.74412145947787	25.971572864334874	30.23194726407299
22	15.662512242834477	31.99240746265111	23.395231696467462	28.949848598046955
23	16.241588413724646	22.866578843854032	40.36584625375865	20.525986488662674
24	16.165425443448004	23.66780801123569	36.308881103927476	23.85788544138883
25	14.461856475700449	32.822280242771114	29.526284804629444	23.18957847689899
26	15.866449485602955	31.55245741077581	29.00383586813906	23.577257235482175
27	17.672713064919364	26.825073325008645	38.77077981905684	16.731433791015146
28	14.177400321547653	23.90830875966557	34.23175718664284	27.682533732143945
29	16.97708246984501	29.015055716446714	30.895898287455154	23.111963526253128
30	24.897239389323456	24.798240727785362	26.488741872209886	23.815778010681296
31	25.377448896890915	24.09310626120335	26.056711713257634	24.4727331286481
32	22.014794359980254	27.566771297252064	27.443088969437134	22.97534537333055
33	15.678880021542108	24.505468686063363	30.0123022336738	29.803349058720723
34	15.505170370096597	24.556024002555485	31.684983619021473	28.253822008326445
35	25.500207237198154	22.082245448041544	25.48291547098283	26.934631843777474
36	20.159295446325565	27.82957974408186	27.522023902236842	24.48910090735573
37	17.463627891750903	26.93621582236208	26.99746299430032	28.6026932915867
38	18.926300116422425	26.998914974669542	26.13195069602659	27.94283421288144
39	25.598017914797794	22.240907302933262	26.18356199824178	25.977512784027162
40	16.416618047324	19.9352944748187	32.13430554418904	31.513781933668255
41	20.876837745153686	22.88321061899243	26.2217094824878	30.01824215336609
42	21.564680445520377	22.256483092348592	28.649552658048062	27.529283804082972
43	20.755003392354133	28.17805503269596	27.860995319343285	23.205946255606623
44	15.10521577748269	33.23107871581576	26.48689389719451	25.176811609507038
45	20.40507612337081	27.515423991467635	26.49138183651757	25.58811804864398
46	21.97189493998041	23.06576415086868	27.684249708943938	27.278091200206973
47	21.006195996230133	20.203646846694635	31.825561718405567	26.96459543866967
48	20.321653251248044	18.79139794029985	29.765597569120867	31.121351239331247
49	19.406641622205267	25.53175481067496	30.012038237243033	25.04956532987674
50	18.579936799254472	26.452574361194635	26.994559033561867	27.972929805989022
51	18.9198322038686	20.973988431676403	31.744646812375098	28.361532552079893
52	21.375395004659538	19.643050425958243	33.27015018756946	25.71140438181276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	187.5
2	287.0
3	1420.5
4	2554.0
5	1763.5
6	973.0
7	1075.0
8	1177.0
9	1232.5
10	1288.0
11	1301.0
12	1314.0
13	1277.0
14	1177.0
15	1114.0
16	1098.5
17	1083.0
18	1055.5
19	1028.0
20	1237.5
21	1447.0
22	1446.5
23	1446.0
24	1801.0
25	2156.0
26	2969.5
27	3783.0
28	5568.5
29	7354.0
30	7685.5
31	8017.0
32	8980.5
33	9944.0
34	11403.5
35	12863.0
36	15192.0
37	17521.0
38	18797.0
39	22552.0
40	25031.0
41	33604.0
42	42177.0
43	57055.0
44	71933.0
45	76925.5
46	81918.0
47	78801.0
48	75684.0
49	80935.5
50	86187.0
51	85894.5
52	85602.0
53	74949.5
54	64297.0
55	52321.5
56	40346.0
57	33015.0
58	25684.0
59	22188.0
60	18692.0
61	17367.0
62	16042.0
63	14124.0
64	8978.0
65	5750.0
66	4609.0
67	3468.0
68	2606.0
69	1744.0
70	1434.0
71	1124.0
72	1134.5
73	1145.0
74	992.0
75	839.0
76	641.0
77	443.0
78	389.5
79	336.0
80	255.0
81	174.0
82	91.0
83	8.0
84	5.5
85	3.0
86	2.5
87	2.0
88	1.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	757586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.637403063999002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.28858471582356	15.4587621688214
2	12.821656969248071	5.804980335224009
3	5.121952192039272	3.478430887371786
4	2.627488565230578	2.379180707886921
5	1.4991644845117647	1.6968595347562552
6	0.9635557143442944	1.3087439448138767
7	0.6412027313474494	1.0160615272674496
8	0.4666578167749084	0.8451136873039518
9	0.35193488092004555	0.7170202576500806
>10	5.2403731124058845	31.98709320022262
>50	1.7448588796157214	25.397137190582715
>100	0.2238047390799799	7.507337348964752
>500	0.004674772617858587	0.7337620967121998
>1k	0.004090426040626263	1.6695171124219712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2933	0.38715076572164747	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	2479	0.3272235759372533	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2291	0.30240791144503726	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	0.19298139089159513	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1356	0.1789895800608776	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1064	0.1404461011687122	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1036	0.13675015113795663	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	891	0.11761040990725805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33237150633723433	0.0
2	0.0	0.0	0.0	1.2605829569184224	0.0
3	0.0	0.0	0.0	2.0298685561771204	0.0
4	0.0	0.0	0.0	2.484206413529289	0.0
5	0.0	0.0	0.0	5.00748429881228	0.0
6	0.0	0.0	0.0	5.473965991979789	0.0
7	0.0	0.0	0.0	7.895473253201617	0.0
8	0.0	0.0	0.0	10.459670585253688	0.0
9	0.0	0.0	0.0	12.921305303952291	0.0
10	0.0	0.0	0.0	13.970823114471493	0.0
11	0.0	0.0	0.0	15.027468828621437	0.0
12	0.0	0.0	0.0	15.88743720184903	0.0
13	0.0	0.0	0.0	16.193805059755594	0.0
14	0.0	0.0	0.0	16.430873854585485	0.0
15	0.0	0.0	0.0	17.311169952982237	0.0
16	0.0	0.0	0.0	18.289276728978624	0.0
17	0.0	0.0	0.0	19.773728659188528	0.0
18	0.0	0.0	0.0	20.351220851494087	0.0
19	0.0	0.0	0.0	21.04170351616846	0.0
20	0.0	0.0	0.0	21.883324137457663	0.0
21	7.919892923047681E-4	0.0	0.0	22.583970664716613	0.0
22	7.919892923047681E-4	0.0	0.0	23.471130670313336	0.0
23	9.23987507688896E-4	0.0	0.0	24.06657461991114	0.0
24	9.23987507688896E-4	0.0	0.0	24.536752263109403	0.0
25	9.23987507688896E-4	0.0	0.0	25.031217577938346	0.0
26	9.23987507688896E-4	0.0	0.0	25.460343776152147	0.0
27	9.23987507688896E-4	0.0	0.0	25.932897387227325	0.0
28	9.23987507688896E-4	0.0	0.0	26.362023585441126	0.0
29	9.23987507688896E-4	0.0	0.0	27.016998730177168	0.0
30	9.23987507688896E-4	0.0	0.0	27.597394883221178	0.0
31	9.23987507688896E-4	0.0	0.0	28.04896077805028	0.0
32	9.23987507688896E-4	0.0	0.0	28.473995031587172	0.0
33	9.23987507688896E-4	0.0	0.0	28.903253228016357	0.0
34	0.0010559857230730242	0.0	0.0	29.561528328137005	0.0
35	0.0010559857230730242	0.0	0.0	30.00741829970459	0.0
36	0.0010559857230730242	0.0	0.0	30.486439823333587	0.0
37	0.0010559857230730242	0.0	0.0	30.942361659270368	0.0
38	0.0010559857230730242	0.0	0.0	31.348256171576562	0.0
39	0.0010559857230730242	0.0	0.0	31.817113832620983	0.0
40	0.0010559857230730242	0.0	0.0	32.27118769354238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	6.310084E-4	46.000004	13
AACGTCC	20	6.310084E-4	46.000004	38
AATCCGG	40	5.6061253E-9	46.000004	1
CGAACGG	20	6.310084E-4	46.000004	18
TCCGCGA	20	6.310084E-4	46.000004	24
TCCGCCT	20	6.310084E-4	46.000004	46
GATCGTA	20	6.310084E-4	46.000004	8
ATAGGCG	20	6.310084E-4	46.000004	13
CCGATTC	20	6.310084E-4	46.000004	10
CTATCGG	40	5.6061253E-9	46.000004	1
GCGCGTC	20	6.310084E-4	46.000004	33
TTTAACG	20	6.310084E-4	46.000004	35
TTTCGAG	20	6.310084E-4	46.000004	13
ACTCTTA	20	6.310084E-4	46.000004	39
GGCCGGC	20	6.310084E-4	46.000004	8
TACGGGT	40	5.6061253E-9	46.000004	3
ATCACGG	40	5.6061253E-9	46.000004	1
CCGTCCG	20	6.310084E-4	46.000004	21
AACGGTC	20	6.310084E-4	46.000004	20
ATCCGGA	20	6.310084E-4	46.000004	9
>>END_MODULE