FastQCFastQC Report
Thu 26 May 2016
SRR1544947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544947_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences475129
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT26590.5596374879243322No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT19160.40325890442385115Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT17060.35906038149639363No Hit
TGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTTG11220.236146393926702Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.19868288401676176No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC7480.157430929284468No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT7300.15364248446211448No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT6050.12733383986243738No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC6050.12733383986243738No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC5570.11723132033616132No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC5090.10712880080988531No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT4900.10312988683073439No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAC206.307697E-446.00000423
GCGACGG206.307697E-446.0000041
GCGAAGG1100.046.0000041
TCGGGGT405.5988494E-946.0000044
GCACGCG206.307697E-446.0000048
TGACGCA206.307697E-446.00000410
GGGCGCG206.307697E-446.0000046
GGGCGAA206.307697E-446.0000046
GGAACGG405.5988494E-946.0000041
ACTACGG405.5988494E-946.0000041
GACGGTG206.307697E-446.0000048
GGACGGT206.307697E-446.0000047
GGGCCGT206.307697E-446.0000046
TTGCCGA206.307697E-446.00000432
GACGCAC206.307697E-446.00000411
GGACCGG206.307697E-446.0000041
ATACGTC206.307697E-446.00000425
TTCGACC206.307697E-446.00000424
ATCGCGG800.046.0000041
CTCGTCC1000.046.036
Produced by FastQC (version 0.11.5)