##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544947_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	475129
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96786556914017	34.0	33.0	34.0	31.0	34.0
2	33.30439733209297	34.0	34.0	34.0	31.0	34.0
3	33.425476028615385	34.0	34.0	34.0	31.0	34.0
4	36.653428858267965	37.0	37.0	37.0	35.0	37.0
5	36.642596010767605	37.0	37.0	37.0	35.0	37.0
6	36.44709331571005	37.0	37.0	37.0	35.0	37.0
7	36.29707090074485	37.0	37.0	37.0	35.0	37.0
8	36.437422257955205	37.0	37.0	37.0	35.0	37.0
9	38.322436643522074	39.0	39.0	39.0	37.0	39.0
10	38.1765394240301	39.0	39.0	39.0	35.0	39.0
11	37.453438960787494	39.0	35.0	39.0	35.0	39.0
12	37.415295635501096	39.0	35.0	39.0	35.0	39.0
13	37.41258058337841	39.0	35.0	39.0	35.0	39.0
14	38.64696114107958	41.0	35.0	41.0	35.0	41.0
15	38.68573587383637	41.0	35.0	41.0	35.0	41.0
16	38.72041908618502	41.0	35.0	41.0	35.0	41.0
17	38.83292326925951	41.0	36.0	41.0	35.0	41.0
18	38.83090697473739	41.0	36.0	41.0	35.0	41.0
19	38.760349294612624	41.0	35.0	41.0	35.0	41.0
20	38.68046362145859	41.0	35.0	41.0	35.0	41.0
21	38.65092637999364	41.0	35.0	41.0	35.0	41.0
22	38.627797924353175	41.0	35.0	41.0	35.0	41.0
23	38.58982297439222	41.0	35.0	41.0	35.0	41.0
24	38.564493011371646	41.0	35.0	41.0	35.0	41.0
25	38.54110357397675	40.0	35.0	41.0	35.0	41.0
26	38.4784048121668	40.0	35.0	41.0	35.0	41.0
27	38.547282948420325	41.0	35.0	41.0	35.0	41.0
28	38.55531866082685	40.0	36.0	41.0	35.0	41.0
29	38.57547529197334	40.0	36.0	41.0	35.0	41.0
30	38.4822627118109	40.0	35.0	41.0	35.0	41.0
31	38.40093742962438	40.0	35.0	41.0	35.0	41.0
32	38.34557351792881	40.0	35.0	41.0	35.0	41.0
33	38.28618333126372	40.0	35.0	41.0	35.0	41.0
34	38.14064180464674	40.0	35.0	41.0	35.0	41.0
35	38.10137667875461	40.0	35.0	41.0	35.0	41.0
36	38.0269968787424	40.0	35.0	41.0	35.0	41.0
37	37.96256806046358	40.0	35.0	41.0	35.0	41.0
38	37.964192882354055	40.0	35.0	41.0	35.0	41.0
39	37.92050369478605	40.0	35.0	41.0	35.0	41.0
40	37.910439059708	40.0	35.0	41.0	35.0	41.0
41	37.88599096245441	40.0	35.0	41.0	35.0	41.0
42	37.82488334746984	40.0	35.0	41.0	35.0	41.0
43	37.755546388454505	40.0	35.0	41.0	35.0	41.0
44	37.62941011809424	40.0	35.0	41.0	35.0	41.0
45	37.64027243127656	40.0	35.0	41.0	35.0	41.0
46	37.58918525284712	40.0	35.0	41.0	35.0	41.0
47	37.53385291152508	39.0	35.0	41.0	35.0	41.0
48	37.510766549715974	39.0	35.0	41.0	35.0	41.0
49	37.43850196472958	39.0	35.0	41.0	35.0	41.0
50	37.37428572029912	39.0	35.0	41.0	35.0	41.0
51	37.31578371347571	39.0	35.0	41.0	35.0	41.0
52	36.88376419877549	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	3.0
15	3.0
16	8.0
17	12.0
18	24.0
19	50.0
20	96.0
21	156.0
22	248.0
23	402.0
24	805.0
25	1354.0
26	1904.0
27	2288.0
28	2095.0
29	1937.0
30	2120.0
31	2619.0
32	3735.0
33	5690.0
34	20385.0
35	105409.0
36	13279.0
37	19996.0
38	41599.0
39	247395.0
40	1514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.900660662683187	22.29773387858876	24.517131137017525	22.284474321710526
2	25.397102681587526	24.75643456829619	27.326894380263045	22.51956836985324
3	25.163481917542395	25.114442604008598	27.435496465170512	22.286579013278498
4	25.683551203988813	27.726785778178137	22.880312504604014	23.709350513229037
5	22.354560550924067	35.92918975688708	21.014082491281314	20.702167200907542
6	86.64930997686943	2.600135963075291	7.735162450618674	3.015391609436595
7	86.92376175733327	1.9468397003761084	5.651096860010649	5.47830168227997
8	76.91342772173452	4.225168322708149	11.79763811512242	7.063765840434913
9	49.13444559267062	21.704842263890438	14.515426336847467	14.645285806591474
10	40.389873066051535	18.41689309640119	29.50693390637069	11.686299931176586
11	39.37898970595354	17.255945227506636	25.500863975888656	17.86420109065117
12	18.358172201654707	35.906038149639365	28.93003794758897	16.80575170111696
13	12.668980424263726	38.81619518067725	28.931932170000145	19.582892225058878
14	10.826954363972732	23.134138307701697	47.667896508106224	18.371010820219354
15	12.429676992985064	18.277772983758094	49.3644883810502	19.928061642206643
16	12.90323259577925	21.41060638268765	29.60585441006548	36.08030661146762
17	16.906145488909328	22.156719543534493	36.36843888712329	24.568696080432893
18	22.71172671000928	28.09973712402316	28.496681953743085	20.69185421222447
19	25.394156113392363	23.54223800273189	26.181731698128296	24.88187418574745
20	17.467677199244836	32.69070084124522	27.7135262212999	22.128095738210042
21	18.26451342687986	24.95511745231295	25.962001898431797	30.818367222375397
22	15.350778420176415	32.678704099307765	23.192227794977786	28.778289685538034
23	15.450961738811985	23.252421973821846	39.8712770637027	21.425339223663467
24	16.39365309210762	23.66199495294962	35.78165087797209	24.162701076970674
25	14.246446754460369	32.16831639407403	30.246732992513614	23.338503858951988
26	16.21349149388903	29.867888510278263	28.906886340341252	25.011733655491458
27	18.133812080508662	26.048083783561935	38.57520799614421	17.242896139785195
28	14.448707614142686	23.6634682370472	33.816500360954606	28.071323787855505
29	17.131347486682564	28.242645681488604	30.422895676753047	24.203111155075778
30	24.41821063332274	23.98780120767202	26.0672364768305	25.526751682174737
31	25.53601232507382	23.185703251117065	25.41604490569929	25.86223951810982
32	21.264119849556646	26.470916319567948	28.155511450574476	24.109452380300926
33	15.149990844591679	22.867052947725774	30.59590132364053	31.38705488404202
34	15.703524726968887	23.576544475289868	31.881657402515952	28.838273395225293
35	24.320132006255143	21.546358988821986	25.325753637433202	28.807755367489673
36	20.326479756024156	27.260807065028654	26.27454859627596	26.13816458267123
37	17.2018546542097	26.396410238061662	26.943419576578147	29.458315531150486
38	19.00515438964997	26.265077484220072	24.92081097975497	29.808957146374986
39	25.86223951810982	21.460698042005436	25.96410658999977	26.712955849884978
40	17.008223029956074	18.919282973676623	31.676239505481668	32.39625449088563
41	21.044810988173737	22.603966501729005	25.71933096064437	30.631891549452885
42	21.10353188292022	21.76650972683208	28.816384602918365	28.31357378732934
43	21.766088788518488	27.176198463996094	27.60829164290119	23.44942110458423
44	15.149569906278085	31.328544458452335	27.539889166942032	25.98199646832755
45	19.91859053015076	26.868071618444677	26.706852244337853	26.50648560706671
46	22.4903131570584	21.390822281948694	27.893687819518487	28.22517674147442
47	20.619031883972564	18.915494528854268	32.547160876309384	27.918312710863784
48	20.041925456034047	17.777487798050633	29.58859593920809	32.59199080670723
49	19.22909357248242	24.08903687209158	30.648308143683085	26.033561411742916
50	18.827939359626544	25.04162027575669	27.829494726695277	28.300945637921494
51	18.654723243582268	19.686022111889613	32.10728033860278	29.551974305925338
52	21.274222369082924	18.464459125837404	34.17703402654858	26.084284478531096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	125.5
2	203.0
3	925.5
4	1648.0
5	1163.0
6	678.0
7	736.0
8	794.0
9	830.0
10	866.0
11	854.5
12	843.0
13	847.0
14	774.0
15	697.0
16	683.5
17	670.0
18	666.0
19	662.0
20	743.0
21	824.0
22	846.0
23	868.0
24	1032.5
25	1197.0
26	1616.0
27	2035.0
28	2915.0
29	3795.0
30	3972.0
31	4149.0
32	4752.0
33	5355.0
34	6196.0
35	7037.0
36	8489.0
37	9941.0
38	10685.0
39	12810.5
40	14192.0
41	19222.5
42	24253.0
43	33087.0
44	41921.0
45	44955.0
46	47989.0
47	47365.5
48	46742.0
49	52626.5
50	58511.0
51	59131.5
52	59752.0
53	51890.5
54	44029.0
55	35572.0
56	27115.0
57	21929.5
58	16744.0
59	14264.0
60	11784.0
61	10849.5
62	9915.0
63	8857.0
64	5754.0
65	3709.0
66	2980.0
67	2251.0
68	1707.0
69	1163.0
70	947.5
71	732.0
72	726.0
73	720.0
74	650.5
75	581.0
76	441.5
77	302.0
78	266.5
79	231.0
80	163.0
81	95.0
82	51.0
83	7.0
84	3.5
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	475129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.35340875652515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.89375641403268	19.11832488722037
2	11.999207025845868	6.564384290642608
3	4.478289732540771	3.6748946875301227
4	2.1246817041183834	2.3246914852104226
5	1.2504750410878591	1.7102377469354395
6	0.8099064890120661	1.3292221949105512
7	0.5429681012225649	1.0396419890146598
8	0.5096648179268589	1.115285607485867
9	0.4107979650027252	1.011305218776143
>10	7.49224909253947	48.5207675572822
>50	0.3999776478409339	7.454285013207038
>100	0.07953223499539806	3.581089802479963
>500	0.00540510334920181	0.9922713000324898
>1k	0.003088630485258177	1.5635982192721418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2659	0.5596374879243322	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	1916	0.40325890442385115	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1706	0.35906038149639363	No Hit
TGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTTG	1122	0.236146393926702	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	944	0.19868288401676176	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	748	0.157430929284468	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	730	0.15364248446211448	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	605	0.12733383986243738	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	605	0.12733383986243738	No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	557	0.11723132033616132	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	509	0.10712880080988531	No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	490	0.10312988683073439	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40978344828457114	0.0
2	0.0	0.0	0.0	1.6012493449147496	0.0
3	0.0	0.0	0.0	2.540994130015217	0.0
4	0.0	0.0	0.0	3.093054728294842	0.0
5	0.0	0.0	0.0	6.131177006665558	0.0
6	0.0	0.0	0.0	6.712071879426429	0.0
7	0.0	0.0	0.0	9.777134209867215	0.0
8	0.0	0.0	0.0	12.806837721966035	0.0
9	0.0	0.0	0.0	15.809601181994784	0.0
10	0.0	0.0	0.0	17.081887234835172	0.0
11	0.0	0.0	0.0	18.282824243521233	0.0
12	0.0	0.0	0.0	19.335801434978713	0.0
13	0.0	0.0	0.0	19.696545569729484	0.0
14	0.0	0.0	0.0	19.98131033887639	0.0
15	0.0	0.0	0.0	21.022080319239617	0.0
16	0.0	0.0	0.0	22.126411984955666	0.0
17	0.0	0.0	0.0	23.669992780907922	0.0
18	0.0	0.0	0.0	24.316343561432788	0.0
19	0.0	0.0	0.0	25.043304029011068	0.0
20	0.0	0.0	0.0	25.95168890974872	0.0
21	6.314074703922514E-4	0.0	0.0	26.706220836867463	0.0
22	6.314074703922514E-4	0.0	0.0	27.673326612351595	0.0
23	6.314074703922514E-4	0.0	0.0	28.313363318172538	0.0
24	6.314074703922514E-4	0.0	0.0	28.824592900033466	0.0
25	6.314074703922514E-4	0.0	0.0	29.347608754675047	0.0
26	6.314074703922514E-4	0.0	0.0	29.797591811907925	0.0
27	6.314074703922514E-4	0.0	0.0	30.28419650242355	0.0
28	6.314074703922514E-4	0.0	0.0	30.72997017652048	0.0
29	6.314074703922514E-4	0.0	0.0	31.39736787272509	0.0
30	6.314074703922514E-4	0.0	0.0	32.03193238046931	0.0
31	6.314074703922514E-4	0.0	0.0	32.51895800929853	0.0
32	6.314074703922514E-4	0.0	0.0	32.98177968509605	0.0
33	6.314074703922514E-4	0.0	0.0	33.413662394844344	0.0
34	6.314074703922514E-4	0.0	0.0	34.0854799433417	0.0
35	6.314074703922514E-4	0.0	0.0	34.55650991625432	0.0
36	6.314074703922514E-4	0.0	0.0	35.02627707422616	0.0
37	6.314074703922514E-4	0.0	0.0	35.46342151289439	0.0
38	6.314074703922514E-4	0.0	0.0	35.879729505039684	0.0
39	6.314074703922514E-4	0.0	0.0	36.36443997314414	0.0
40	6.314074703922514E-4	0.0	0.0	36.827472118098456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAC	20	6.307697E-4	46.000004	23
GCGACGG	20	6.307697E-4	46.000004	1
GCGAAGG	110	0.0	46.000004	1
TCGGGGT	40	5.5988494E-9	46.000004	4
GCACGCG	20	6.307697E-4	46.000004	8
TGACGCA	20	6.307697E-4	46.000004	10
GGGCGCG	20	6.307697E-4	46.000004	6
GGGCGAA	20	6.307697E-4	46.000004	6
GGAACGG	40	5.5988494E-9	46.000004	1
ACTACGG	40	5.5988494E-9	46.000004	1
GACGGTG	20	6.307697E-4	46.000004	8
GGACGGT	20	6.307697E-4	46.000004	7
GGGCCGT	20	6.307697E-4	46.000004	6
TTGCCGA	20	6.307697E-4	46.000004	32
GACGCAC	20	6.307697E-4	46.000004	11
GGACCGG	20	6.307697E-4	46.000004	1
ATACGTC	20	6.307697E-4	46.000004	25
TTCGACC	20	6.307697E-4	46.000004	24
ATCGCGG	80	0.0	46.000004	1
CTCGTCC	100	0.0	46.0	36
>>END_MODULE