FastQCFastQC Report
Thu 26 May 2016
SRR1544946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544946_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences975156
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT32500.3332800085319682No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT27230.2792373733023229No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT22530.23103995668385366No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT21350.21893932868176988Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.2065310575948874No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18160.18622661399817056No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11810.1211088277157706No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA11610.11905787381711234No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11120.1140330367653996No Hit
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT10710.10982858127315015No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC10390.10654705503529692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG206.3109805E-446.00000413
TCCGCTT206.3109805E-446.00000419
GACGTAG206.3109805E-446.00000416
CACGCTA206.3109805E-446.00000425
TAAACGG405.6097633E-946.0000041
CGTAGTC206.3109805E-446.00000418
GTTCGAC206.3109805E-446.00000412
TACGAGT206.3109805E-446.00000430
ACGTAGG700.046.0000041
TCGGTAA206.3109805E-446.00000413
TACCGCC206.3109805E-446.00000414
CGAAGCA206.3109805E-446.00000431
GTTCGAT301.8611099E-646.038
GCGTATG253.416772E-546.025
AGTACGG301.8611099E-646.01
ACCGGGT950.045.9999963
CTAACGG1450.044.413791
TTAGCGG2850.044.3859631
AATGCGG1400.044.3571431
CCGGGTA850.043.2941174
Produced by FastQC (version 0.11.5)