##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544946_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	975156
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.936509645636185	34.0	33.0	34.0	31.0	34.0
2	33.259422082210435	34.0	33.0	34.0	31.0	34.0
3	33.31847212138366	34.0	34.0	34.0	31.0	34.0
4	36.58901242467872	37.0	37.0	37.0	35.0	37.0
5	36.58245655054166	37.0	37.0	37.0	35.0	37.0
6	36.66089733334974	37.0	37.0	37.0	35.0	37.0
7	36.50802333165155	37.0	37.0	37.0	35.0	37.0
8	36.541464134969175	37.0	37.0	37.0	35.0	37.0
9	38.34172583668664	39.0	39.0	39.0	37.0	39.0
10	38.16770752577023	39.0	39.0	39.0	35.0	39.0
11	37.545207125834224	39.0	35.0	39.0	35.0	39.0
12	37.47225264470505	39.0	35.0	39.0	35.0	39.0
13	37.45872045088171	39.0	35.0	39.0	35.0	39.0
14	38.797819015624164	41.0	35.0	41.0	35.0	41.0
15	38.862972693599794	41.0	35.0	41.0	35.0	41.0
16	38.87037458622005	41.0	36.0	41.0	35.0	41.0
17	38.92413521528863	41.0	36.0	41.0	35.0	41.0
18	38.90653495440729	41.0	36.0	41.0	35.0	41.0
19	38.8269712743397	41.0	36.0	41.0	35.0	41.0
20	38.74975286005521	41.0	35.0	41.0	35.0	41.0
21	38.7045231737281	41.0	35.0	41.0	35.0	41.0
22	38.66848893920562	41.0	35.0	41.0	35.0	41.0
23	38.63102416433883	41.0	35.0	41.0	35.0	41.0
24	38.58250064605048	41.0	35.0	41.0	35.0	41.0
25	38.54973563204246	40.0	35.0	41.0	35.0	41.0
26	38.496944078691	40.0	35.0	41.0	35.0	41.0
27	38.47321044017573	40.0	36.0	41.0	35.0	41.0
28	38.48558179409243	40.0	36.0	41.0	35.0	41.0
29	38.496251881750204	40.0	36.0	41.0	35.0	41.0
30	38.43377572408927	40.0	35.0	41.0	35.0	41.0
31	38.368092900007795	40.0	35.0	41.0	35.0	41.0
32	38.30987965002522	40.0	35.0	41.0	35.0	41.0
33	38.216613546960694	40.0	35.0	41.0	35.0	41.0
34	38.148812087501895	40.0	35.0	41.0	35.0	41.0
35	38.07751785355369	40.0	35.0	41.0	35.0	41.0
36	38.01399160749665	40.0	35.0	41.0	35.0	41.0
37	37.980830759386194	40.0	35.0	41.0	35.0	41.0
38	37.925806742716034	40.0	35.0	41.0	35.0	41.0
39	37.83562937622288	40.0	35.0	41.0	35.0	41.0
40	37.75737317926568	40.0	35.0	41.0	35.0	41.0
41	37.71523735689469	40.0	35.0	41.0	35.0	41.0
42	37.640900532837826	40.0	35.0	41.0	35.0	41.0
43	37.570112884502585	40.0	35.0	41.0	35.0	41.0
44	37.5125415830903	39.0	35.0	41.0	35.0	41.0
45	37.43301789662372	39.0	35.0	41.0	35.0	41.0
46	37.436783447981654	39.0	35.0	41.0	35.0	41.0
47	37.41191050457568	39.0	35.0	41.0	35.0	41.0
48	37.40742917030711	39.0	35.0	41.0	35.0	41.0
49	37.36496622078929	39.0	35.0	41.0	35.0	41.0
50	37.29559680707497	39.0	35.0	41.0	35.0	41.0
51	37.22129690018828	39.0	35.0	41.0	34.0	41.0
52	37.00357173621451	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	6.0
14	19.0
15	22.0
16	21.0
17	33.0
18	73.0
19	146.0
20	239.0
21	407.0
22	616.0
23	1029.0
24	1776.0
25	2900.0
26	4101.0
27	4735.0
28	4772.0
29	5301.0
30	6061.0
31	7444.0
32	9861.0
33	13783.0
34	42898.0
35	184386.0
36	33955.0
37	52031.0
38	96423.0
39	497192.0
40	4922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18156274483262	22.08087731603969	23.960679111854922	20.776880827272766
2	26.710598099175925	24.436602964038574	25.972152147964017	22.880646788821483
3	26.81837572655042	25.33502331934583	25.09885597791533	22.747744976188425
4	25.96692221552244	27.922814400977895	22.57085020242915	23.539413181070515
5	23.294529285570718	35.55369602402077	20.678127397052368	20.47364729335614
6	87.38314690162395	3.245839640016572	6.776146585777044	2.594866872582438
7	87.79015870281268	2.779657818851548	5.156508292006612	4.273675186329162
8	78.17836325675071	5.114566284779051	10.283175204787746	6.423895253682488
9	48.677955116924885	22.495067455873727	14.886746325716091	13.9402311014853
10	37.97628276911592	21.520146520146522	26.22113795126113	14.282432759476432
11	37.91321593673217	17.769156934890418	25.132696717243192	19.184930411134218
12	20.856560386235635	33.68332861613937	27.12222454663664	18.337886450988357
13	14.553876507963853	35.38141589653348	28.185746690785884	21.878960904716784
14	12.90921657662979	24.491568528522617	42.32379229579678	20.27542259905082
15	15.3563122208139	18.786635164014783	44.03428784727777	21.822764767893545
16	15.125579907214846	22.59279540914479	28.145958185151915	34.13566649848845
17	17.98358416499514	22.969042901853655	33.151721365607145	25.895651567544064
18	22.262284188376015	27.499907707074563	26.805967455463538	23.43184064908589
19	25.202326602102637	22.84721624027335	27.048800397064674	24.901656760559337
20	19.115403073969702	30.92212938237574	27.139555107080305	22.82291243657425
21	20.99356410666601	25.314923971138974	24.975696196300902	28.71581572589411
22	18.349884531295505	30.026067624051944	24.208229247422977	27.415818597229567
23	17.86616705429695	22.087337820820462	37.8749656465222	22.171529478360384
24	19.024238173174343	23.260278355463125	33.12690482343338	24.58857864792915
25	17.10187908396195	30.082981594739717	28.118577950604827	24.69656137069351
26	17.488894084638765	29.06488807944575	27.443096284081726	26.003121551833758
27	19.197031039136302	25.554372838807325	34.82653031925149	20.422065802804884
28	16.135059416134446	24.417631640475985	32.580428157135884	26.866880786253688
29	18.407516335847802	28.0032117938053	28.263170200460237	25.326101669886665
30	24.55996784104287	25.929594854566858	25.317077472732567	24.193359831657705
31	25.14500244063514	26.90707948266739	24.24740246688735	23.70051560981012
32	22.222290587352177	26.271181226388396	25.051786585941123	26.45474160031831
33	16.70050740599453	27.89122971093856	27.28763397856343	28.120628904503487
34	17.59995323825111	24.902887332898533	30.860498217721062	26.636661211129297
35	24.035436381461018	25.06983498024931	24.44388385037881	26.45084478791086
36	18.52862516356357	29.80169326753873	28.98541361587274	22.684267953024953
37	18.641427627989778	27.864362214866134	29.864042266057943	23.630167891086142
38	17.31548593250721	29.83891808079938	26.713366886939117	26.132229099754294
39	23.24058919803601	24.557814339449276	27.12960798067181	25.071988481842904
40	15.83110804835329	22.596282030772514	31.96473179675867	29.607878124115526
41	19.596659406289866	24.908014717645177	25.713321765953346	29.782004110111615
42	20.07668516627083	24.575247447587873	27.67557190849464	27.672495477646653
43	20.978592143205805	28.687922752872364	26.413825070040076	23.919660033881758
44	16.79885064543519	30.97688985146992	25.916776392700246	26.307483110394646
45	20.235428998026983	27.767864833934265	26.8145814618379	25.182124706200852
46	21.17415059744287	24.587245527895025	28.343567593287638	25.895036281374466
47	20.034743159043273	22.209061934705833	31.380415031030935	26.375779875219962
48	18.536316240683544	22.623252074539867	29.268752896972384	29.571678787804206
49	18.552108585703213	25.824278371870758	30.557982517668968	25.065630524757065
50	17.680965917248113	26.668656091948368	28.41678664746974	27.233591343333785
51	18.372650119570615	22.84721624027335	30.329506253358435	28.4506273867976
52	20.981053287884194	21.526709572622227	31.481219415149987	26.011017724343592
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	288.0
2	428.0
3	1981.5
4	3535.0
5	2363.0
6	1191.0
7	1303.5
8	1416.0
9	1559.0
10	1702.0
11	1584.0
12	1466.0
13	1434.0
14	1325.5
15	1249.0
16	1170.0
17	1091.0
18	1127.0
19	1163.0
20	1269.5
21	1376.0
22	1345.0
23	1314.0
24	1966.5
25	2619.0
26	3725.5
27	4832.0
28	7510.5
29	10189.0
30	9817.5
31	9446.0
32	11608.0
33	13770.0
34	16946.5
35	20123.0
36	22959.5
37	25796.0
38	26611.5
39	30465.5
40	33504.0
41	44032.0
42	54560.0
43	73579.5
44	92599.0
45	106550.0
46	120501.0
47	117578.5
48	114656.0
49	101419.0
50	88182.0
51	78405.0
52	68628.0
53	60369.0
54	52110.0
55	47679.5
56	43249.0
57	39611.5
58	35974.0
59	34360.0
60	32746.0
61	32198.0
62	31650.0
63	29054.0
64	21784.5
65	17111.0
66	14675.5
67	12240.0
68	9646.0
69	7052.0
70	5914.5
71	4777.0
72	4003.0
73	3229.0
74	2533.5
75	1838.0
76	1464.0
77	1090.0
78	954.5
79	819.0
80	550.0
81	281.0
82	210.5
83	140.0
84	97.5
85	55.0
86	35.0
87	15.0
88	9.5
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	975156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.94402438005994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0655898406769	15.375210116670942
2	12.169858002072857	5.341113213987088
3	4.565090626218882	3.0052937999679075
4	2.2666786348645025	1.989602049009105
5	1.2491930848481037	1.3706161754654536
6	0.8028676366190287	1.0570888195157442
7	0.547535969180028	0.8410599859652398
8	0.40600950075949765	0.7127585906561905
9	0.30584459995712426	0.6040315222171933
>10	5.472692224957135	32.921449641504914
>50	1.9376586941829719	27.242383828413026
>100	0.2011572280156867	6.841187383145207
>500	0.004678075070132248	0.6702396750480937
>1k	0.005145882577145474	2.027965198433924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	3250	0.3332800085319682	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2723	0.2792373733023229	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	2253	0.23103995668385366	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT	2135	0.21893932868176988	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.2065310575948874	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1816	0.18622661399817056	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1181	0.1211088277157706	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1161	0.11905787381711234	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1112	0.1140330367653996	No Hit
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	1071	0.10982858127315015	No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1039	0.10654705503529692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2236565226486839	0.0
2	0.0	0.0	0.0	0.9850731575255651	0.0
3	0.0	0.0	0.0	1.6188179122109694	0.0
4	0.0	0.0	0.0	1.9960908818691574	0.0
5	0.0	0.0	0.0	4.15923195878403	0.0
6	0.0	0.0	0.0	4.55239982115682	0.0
7	0.0	0.0	0.0	6.392413111338083	0.0
8	0.0	0.0	0.0	8.337742884215448	0.0
9	0.0	0.0	0.0	10.132122450151565	0.0
10	0.0	0.0	0.0	10.990754299824848	0.0
11	0.0	0.0	0.0	11.853795700380246	0.0
12	0.0	0.0	0.0	12.581371595929268	0.0
13	0.0	0.0	0.0	12.856814704519072	0.0
14	0.0	0.0	0.0	13.075754033200841	0.0
15	0.0	0.0	0.0	13.811533744344494	0.0
16	0.0	0.0	0.0	14.671806357136704	0.0
17	0.0	0.0	0.0	15.805983863094726	0.0
18	0.0	0.0	0.0	16.298315346467643	0.0
19	0.0	0.0	0.0	16.882837207585247	0.0
20	0.0	0.0	0.0	17.583237963977044	0.0
21	0.0	0.0	0.0	18.19278146265828	0.0
22	0.0	0.0	0.0	18.938303204820564	0.0
23	0.0	0.0	0.0	19.48231872643967	0.0
24	1.025476949329133E-4	0.0	0.0	19.929529224042103	0.0
25	2.050953898658266E-4	0.0	0.0	20.37622698316987	0.0
26	2.050953898658266E-4	0.0	0.0	20.782931141273806	0.0
27	2.050953898658266E-4	0.0	0.0	21.191686253276398	0.0
28	2.050953898658266E-4	0.0	0.0	21.610080848602685	0.0
29	3.076430847987399E-4	0.0	0.0	22.139124406761585	0.0
30	3.076430847987399E-4	0.0	0.0	22.628379459286514	0.0
31	3.076430847987399E-4	0.0	0.0	23.05969506417435	0.0
32	3.076430847987399E-4	0.0	0.0	23.45542661892046	0.0
33	3.076430847987399E-4	0.0	0.0	23.86264351549906	0.0
34	3.076430847987399E-4	0.0	0.0	24.427578766884476	0.0
35	3.076430847987399E-4	0.0	0.0	24.826284204783644	0.0
36	3.076430847987399E-4	0.0	0.0	25.247652683262984	0.0
37	3.076430847987399E-4	0.0	0.0	25.647075955026683	0.0
38	4.101907797316532E-4	0.0	0.0	26.01552982292064	0.0
39	4.101907797316532E-4	0.0	0.0	26.436590658315183	0.0
40	4.101907797316532E-4	0.0	0.0	26.844730484148176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	6.3109805E-4	46.000004	13
TCCGCTT	20	6.3109805E-4	46.000004	19
GACGTAG	20	6.3109805E-4	46.000004	16
CACGCTA	20	6.3109805E-4	46.000004	25
TAAACGG	40	5.6097633E-9	46.000004	1
CGTAGTC	20	6.3109805E-4	46.000004	18
GTTCGAC	20	6.3109805E-4	46.000004	12
TACGAGT	20	6.3109805E-4	46.000004	30
ACGTAGG	70	0.0	46.000004	1
TCGGTAA	20	6.3109805E-4	46.000004	13
TACCGCC	20	6.3109805E-4	46.000004	14
CGAAGCA	20	6.3109805E-4	46.000004	31
GTTCGAT	30	1.8611099E-6	46.0	38
GCGTATG	25	3.416772E-5	46.0	25
AGTACGG	30	1.8611099E-6	46.0	1
ACCGGGT	95	0.0	45.999996	3
CTAACGG	145	0.0	44.41379	1
TTAGCGG	285	0.0	44.385963	1
AATGCGG	140	0.0	44.357143	1
CCGGGTA	85	0.0	43.294117	4
>>END_MODULE