FastQCFastQC Report
Thu 26 May 2016
SRR1544945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544945_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences608458
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT28550.4692189107547275No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT20190.3318224100923975No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT20070.3298502115182971Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA15800.25967281225655675No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.21003914814169589No Hit
TGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTTG12500.20543735146879488Illumina Single End Adapter 2 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11750.1931111103806672No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8270.1359173517317547No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7680.12622070874242758No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC7220.11866061420837593No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7080.11635971587192542No Hit
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT6840.11241531872372455No Hit
GCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC6430.10567697359554809No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC6120.10058212727912198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT351.01850674E-746.0000043
TAAACGG453.092282E-1046.0000041
ACGTAGG750.046.0000041
ACGCGAG206.309102E-446.044
ACAACGT206.309102E-446.08
ATTTACG206.309102E-446.041
TTTACGG301.8599494E-646.01
GGCACTA253.415248E-546.07
TTATGCG206.309102E-446.012
TAGCGCA206.309102E-446.023
CGCGCGA206.309102E-446.027
ACGGGTC301.8599494E-646.04
TCGGCCA206.309102E-446.011
GTAATCG206.309102E-446.020
GCCGATG405.6043064E-946.08
CTAACGG551.8189894E-1246.01
TCGGAGT206.309102E-446.042
GCCGAAC206.309102E-446.010
CCATCAA253.415248E-546.030
CTTGCGT206.309102E-446.015
Produced by FastQC (version 0.11.5)