##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544945_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	608458
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9157986253776	34.0	33.0	34.0	31.0	34.0
2	33.2803381663155	34.0	34.0	34.0	31.0	34.0
3	33.4086247530643	34.0	34.0	34.0	31.0	34.0
4	36.62182106242337	37.0	37.0	37.0	35.0	37.0
5	36.62595610543374	37.0	37.0	37.0	35.0	37.0
6	36.4222049837458	37.0	37.0	37.0	35.0	37.0
7	36.268028360215496	37.0	37.0	37.0	35.0	37.0
8	36.39955099612463	37.0	37.0	37.0	35.0	37.0
9	38.27919264764372	39.0	39.0	39.0	37.0	39.0
10	38.18317616006364	39.0	39.0	39.0	35.0	39.0
11	37.61753646102114	39.0	35.0	39.0	35.0	39.0
12	37.59565820483912	39.0	35.0	39.0	35.0	39.0
13	37.58283069661341	39.0	35.0	39.0	35.0	39.0
14	38.86179161092466	41.0	36.0	41.0	35.0	41.0
15	38.896252494009445	41.0	36.0	41.0	35.0	41.0
16	38.93125080120567	41.0	36.0	41.0	35.0	41.0
17	39.01193837536855	41.0	36.0	41.0	35.0	41.0
18	39.0087845011488	41.0	36.0	41.0	35.0	41.0
19	38.96122164553675	41.0	36.0	41.0	35.0	41.0
20	38.89422277297694	41.0	35.0	41.0	35.0	41.0
21	38.84684398923179	41.0	35.0	41.0	35.0	41.0
22	38.81627491133324	41.0	35.0	41.0	35.0	41.0
23	38.76531165667967	41.0	35.0	41.0	35.0	41.0
24	38.73497266861476	41.0	35.0	41.0	35.0	41.0
25	38.69884856473249	41.0	35.0	41.0	35.0	41.0
26	38.631277754586186	41.0	35.0	41.0	35.0	41.0
27	38.699144394518605	41.0	36.0	41.0	35.0	41.0
28	38.70103934864855	41.0	36.0	41.0	35.0	41.0
29	38.7190751046087	41.0	36.0	41.0	35.0	41.0
30	38.66611006840242	41.0	35.0	41.0	35.0	41.0
31	38.60735827287997	41.0	35.0	41.0	35.0	41.0
32	38.55513774163541	41.0	35.0	41.0	35.0	41.0
33	38.488314723448454	41.0	35.0	41.0	35.0	41.0
34	38.405628654730485	41.0	35.0	41.0	35.0	41.0
35	38.335699423789315	40.0	35.0	41.0	35.0	41.0
36	38.288910656117594	40.0	35.0	41.0	35.0	41.0
37	38.24885037258118	40.0	35.0	41.0	35.0	41.0
38	38.19577193495689	40.0	35.0	41.0	35.0	41.0
39	38.14835370724027	40.0	35.0	41.0	35.0	41.0
40	38.084773312208895	40.0	35.0	41.0	35.0	41.0
41	38.046137942142266	40.0	35.0	41.0	35.0	41.0
42	37.979587744758064	40.0	35.0	41.0	35.0	41.0
43	37.92437111517968	40.0	35.0	41.0	35.0	41.0
44	37.798013996035884	40.0	35.0	41.0	35.0	41.0
45	37.77781868263709	40.0	35.0	41.0	35.0	41.0
46	37.701764131624536	40.0	35.0	41.0	35.0	41.0
47	37.636453132344386	40.0	35.0	41.0	35.0	41.0
48	37.58448405641803	39.0	35.0	41.0	35.0	41.0
49	37.54845856246446	39.0	35.0	41.0	35.0	41.0
50	37.48452152819093	39.0	35.0	41.0	35.0	41.0
51	37.40528845047645	39.0	35.0	41.0	35.0	41.0
52	36.93755033215111	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	2.0
14	5.0
15	5.0
16	15.0
17	24.0
18	50.0
19	80.0
20	134.0
21	239.0
22	343.0
23	564.0
24	971.0
25	1652.0
26	2322.0
27	2737.0
28	2583.0
29	2524.0
30	2889.0
31	3541.0
32	4739.0
33	7284.0
34	25133.0
35	110325.0
36	19973.0
37	32242.0
38	61058.0
39	323742.0
40	3277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15725982730115	21.481186869101894	22.921056178076384	22.44049712552058
2	26.39837096397779	24.653139575780088	26.213641697537053	22.73484776270507
3	27.125947887939677	25.19039933734128	25.32418014061776	22.359472634101284
4	25.915675362966713	27.57511611319105	22.98268738351702	23.526521140325215
5	23.351652866754975	35.28082464196378	20.814912450818298	20.55261004046294
6	86.11013414237301	3.191017292894497	7.4126726906376454	3.2861758740948432
7	86.4156605714774	2.606424765554894	5.951930946753926	5.025983716213773
8	76.1607210357987	5.108158656801291	11.37416222648071	7.356958080919307
9	48.0440720641359	21.720480296092745	15.48422405490601	14.751223584865347
10	37.02819257861677	21.178618737858653	27.213546374605972	14.579642308918611
11	36.46923863274047	17.584944236085317	25.807697491034716	20.1381196401395
12	20.532723704840762	32.26467562263953	28.155764243382453	19.04683642913726
13	14.640123065191023	34.24624213996693	28.78308773982756	22.33054705501448
14	12.974765719244385	23.951036883400334	41.83937100013477	21.234826397220516
15	15.178040226276918	18.48278763694454	43.90508465662379	22.43408748015475
16	15.034069730367586	22.811106107570282	28.57649993919054	33.578324222871586
17	17.62816825483435	23.33341002994455	33.13901699049072	25.899404724730385
18	21.843414007211674	27.65318230674919	27.09439271075407	23.409010975285067
19	24.727425722071203	22.68817239645136	26.88649011106765	25.697911770409792
20	18.749363144210445	31.075932932100493	27.111320748515098	23.063383175173964
21	20.453178362352045	25.101814751387934	25.091953758517434	29.35305312774259
22	18.172988110929598	30.23528328989018	24.32657636188529	27.26515223729493
23	17.572782344878366	22.29110308353247	37.46388411361178	22.67223045797738
24	19.08759519966867	23.359212961289032	32.43329860072512	25.119893238317186
25	17.190668871146407	29.47385029040624	28.360872895088896	24.974607943358457
26	17.62290905863675	28.01146504771077	27.383484151740962	26.98214174191152
27	19.671037277840046	24.96030950369623	34.5447015241808	20.823951694282925
28	16.513547360705257	24.60005456416055	32.042474583290876	26.843923491843313
29	18.75265014183395	26.96554240391284	27.955257388348908	26.326550065904303
30	24.248345818445973	25.612614182079945	24.975594042645508	25.16344595682857
31	24.953406808686875	26.67990231043063	23.583386199211777	24.783304681670714
32	21.45390478882684	25.37545730354437	25.474560281892916	27.696077625735878
33	16.238918709261775	27.790085757767997	27.469439139595504	28.501556393374727
34	17.58642338501589	24.337259104161667	31.318184656952493	26.75813285386995
35	23.348858918775	24.92875432651062	24.532999812641133	27.18938694207324
36	18.550335438107478	29.78496461547058	28.287408498203654	23.377291448218283
37	18.572193972303758	27.790743157292695	29.87338485154275	23.763678018860794
38	17.060339415374603	29.632612275621323	26.25965966426606	27.047388644738007
39	23.033964546443634	24.344654848814546	27.02109266375	25.600287940991816
40	15.866173178756792	22.494403886545992	31.55534153548807	30.08408139920915
41	19.480391415677005	25.060069881569476	25.512360754563172	29.947177948190344
42	19.924957844255477	24.068547048440482	27.697063725022925	28.309431382281108
43	21.55251471753186	28.198823912250308	26.285133895848194	23.963527474369638
44	17.02401809163492	29.597277051168692	26.732658622287815	26.64604623490857
45	20.061861295274284	26.773088693056874	27.207794128764846	25.957255882903997
46	21.400984127088478	23.379427996673556	28.656373981441615	26.563213894796355
47	19.684678317977575	21.549063370027184	31.520006311035438	27.246252000959803
48	18.279322484049846	21.746283227437228	29.248197903552914	30.726196384960012
49	18.588464610540086	24.653139575780088	30.875754776829297	25.88264103685053
50	17.846260547153626	25.637759713899726	28.941685375161473	27.574294363785175
51	18.342761538183407	21.90044341597941	30.49150475464206	29.265290291195118
52	20.84893287622153	21.145091362098945	32.00845415788764	25.997521603791878
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	189.0
2	295.0
3	1273.5
4	2252.0
5	1533.0
6	814.0
7	886.0
8	958.0
9	1022.5
10	1087.0
11	1062.0
12	1037.0
13	975.5
14	847.5
15	781.0
16	749.0
17	717.0
18	723.5
19	730.0
20	738.0
21	746.0
22	781.5
23	817.0
24	1173.5
25	1530.0
26	2090.0
27	2650.0
28	3926.0
29	5202.0
30	5159.5
31	5117.0
32	6301.0
33	7485.0
34	9298.0
35	11111.0
36	12806.0
37	14501.0
38	15205.0
39	17423.0
40	18937.0
41	25240.5
42	31544.0
43	43899.0
44	56254.0
45	66619.0
46	76984.0
47	76550.0
48	76116.0
49	67068.5
50	58021.0
51	51312.5
52	44604.0
53	38951.5
54	33299.0
55	30253.0
56	27207.0
57	24939.0
58	22671.0
59	21439.5
60	20208.0
61	19813.0
62	19418.0
63	18432.5
64	14273.5
65	11100.0
66	9361.0
67	7622.0
68	6040.0
69	4458.0
70	3744.5
71	3031.0
72	2562.5
73	2094.0
74	1629.0
75	1164.0
76	923.5
77	683.0
78	610.0
79	537.0
80	365.0
81	193.0
82	137.0
83	81.0
84	61.5
85	42.0
86	23.0
87	4.0
88	3.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	608458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.464598060462503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43580155030065	18.905197751556713
2	10.948723912703564	5.795071552493483
3	3.9181729421168043	3.110786161335031
4	1.9297503311537643	2.0428026748411514
5	1.0751759809069161	1.4227050089482522
6	0.6964310056264811	1.1058459984449114
7	0.5411811521752661	1.0025499169151486
8	0.4493515225527335	0.9513525945771953
9	0.41804856842846094	0.9957138599889876
>10	8.138706375382322	51.920373845882814
>50	0.36628853773201375	6.406910754041524
>100	0.07176010534783915	3.2433365262617095
>500	0.006240009160681665	1.0885984919102438
>1k	0.004368006412477166	2.0087548628028222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	2855	0.4692189107547275	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	2019	0.3318224100923975	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT	2007	0.3298502115182971	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1580	0.25967281225655675	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.21003914814169589	No Hit
TGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTTG	1250	0.20543735146879488	Illumina Single End Adapter 2 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1175	0.1931111103806672	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	827	0.1359173517317547	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	768	0.12622070874242758	No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	722	0.11866061420837593	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	708	0.11635971587192542	No Hit
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	684	0.11241531872372455	No Hit
GCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	643	0.10567697359554809	No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	612	0.10058212727912198	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.333958958547673	0.0
2	0.0	0.0	0.0	1.3401089311012429	0.0
3	0.0	0.0	0.0	2.0864217415170807	0.0
4	0.0	0.0	0.0	2.5604067988258845	0.0
5	0.0	0.0	0.0	5.033708160629	0.0
6	0.0	0.0	0.0	5.482712035999198	0.0
7	0.0	0.0	0.0	7.704229379842158	0.0
8	0.0	0.0	0.0	10.016303508212564	0.0
9	0.0	0.0	0.0	12.17372439839726	0.0
10	0.0	0.0	0.0	13.183983117980207	0.0
11	0.0	0.0	0.0	14.1919409392267	0.0
12	0.0	0.0	0.0	15.055270865039164	0.0
13	0.0	0.0	0.0	15.385449776319811	0.0
14	0.0	0.0	0.0	15.645122588576369	0.0
15	0.0	0.0	0.0	16.498427171637154	0.0
16	0.0	0.0	0.0	17.458230477699363	0.0
17	0.0	0.0	0.0	18.62642943309152	0.0
18	0.0	0.0	0.0	19.14183066045643	0.0
19	0.0	0.0	0.0	19.740721627458264	0.0
20	0.0	0.0	0.0	20.50199027706103	0.0
21	0.0	0.0	0.0	21.18864408061033	0.0
22	0.0	0.0	0.0	21.97456521238935	0.0
23	0.0	0.0	0.0	22.546995848522002	0.0
24	1.643498811750359E-4	0.0	0.0	23.037580243829485	0.0
25	1.643498811750359E-4	0.0	0.0	23.52668549020639	0.0
26	1.643498811750359E-4	0.0	0.0	23.96763622139901	0.0
27	1.643498811750359E-4	0.0	0.0	24.41302439938336	0.0
28	1.643498811750359E-4	0.0	0.0	24.857097778318305	0.0
29	1.643498811750359E-4	0.0	0.0	25.403396783344125	0.0
30	1.643498811750359E-4	0.0	0.0	25.919784109996087	0.0
31	1.643498811750359E-4	0.0	0.0	26.382922075147338	0.0
32	1.643498811750359E-4	0.0	0.0	26.795275927015506	0.0
33	1.643498811750359E-4	0.0	0.0	27.23606230832695	0.0
34	1.643498811750359E-4	0.0	0.0	27.82246268435948	0.0
35	1.643498811750359E-4	0.0	0.0	28.23103648896062	0.0
36	1.643498811750359E-4	0.0	0.0	28.66360537621331	0.0
37	1.643498811750359E-4	0.0	0.0	29.053278944479324	0.0
38	1.643498811750359E-4	0.0	0.0	29.439172465478308	0.0
39	1.643498811750359E-4	0.0	0.0	29.865989106889877	0.0
40	1.643498811750359E-4	0.0	0.0	30.299708443310795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.01850674E-7	46.000004	3
TAAACGG	45	3.092282E-10	46.000004	1
ACGTAGG	75	0.0	46.000004	1
ACGCGAG	20	6.309102E-4	46.0	44
ACAACGT	20	6.309102E-4	46.0	8
ATTTACG	20	6.309102E-4	46.0	41
TTTACGG	30	1.8599494E-6	46.0	1
GGCACTA	25	3.415248E-5	46.0	7
TTATGCG	20	6.309102E-4	46.0	12
TAGCGCA	20	6.309102E-4	46.0	23
CGCGCGA	20	6.309102E-4	46.0	27
ACGGGTC	30	1.8599494E-6	46.0	4
TCGGCCA	20	6.309102E-4	46.0	11
GTAATCG	20	6.309102E-4	46.0	20
GCCGATG	40	5.6043064E-9	46.0	8
CTAACGG	55	1.8189894E-12	46.0	1
TCGGAGT	20	6.309102E-4	46.0	42
GCCGAAC	20	6.309102E-4	46.0	10
CCATCAA	25	3.415248E-5	46.0	30
CTTGCGT	20	6.309102E-4	46.0	15
>>END_MODULE