FastQCFastQC Report
Thu 26 May 2016
SRR1544944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544944_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1190167
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT41000.3444894708053576Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT36310.30508323621811057Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT35780.30063007964428523Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.20022400217784564No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT15540.1305699116174453Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC15000.1260327332214723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA351.0196163E-746.0000047
AAATCCG351.0196163E-746.00000421
TACCGAA351.0196163E-746.0000048
GTCGCGA206.311545E-446.021
TCGTTAG253.4172303E-546.012
GTTTCGA253.4172303E-546.032
CGACCCA206.311545E-446.035
GCGCCTA253.4172303E-546.033
TTCGCGT206.311545E-446.015
GCGTAAC206.311545E-446.032
GTACGCA253.4172303E-546.08
ATGGGCG1800.045.9999964
CTAACGG900.045.9999961
CGGTCTA1100.043.90909230
ATCGCGG2050.043.7560961
CATGCGG2000.043.6999971
AACGGGA2900.043.6206933
TATCCGG950.043.578951
ACGGGCC900.043.4444434
GCGATAG850.043.2941178