##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544944_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1190167
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92417282616641	34.0	33.0	34.0	31.0	34.0
2	33.231627998423754	34.0	33.0	34.0	31.0	34.0
3	33.28044971840086	34.0	34.0	34.0	31.0	34.0
4	36.572684337576156	37.0	37.0	37.0	35.0	37.0
5	36.566502852120756	37.0	37.0	37.0	35.0	37.0
6	36.67425495749756	37.0	37.0	37.0	35.0	37.0
7	36.5138102467973	37.0	37.0	37.0	35.0	37.0
8	36.54010235538374	37.0	37.0	37.0	35.0	37.0
9	38.3232790020224	39.0	39.0	39.0	37.0	39.0
10	38.152604634475665	39.0	39.0	39.0	35.0	39.0
11	37.61026897905924	39.0	37.0	39.0	35.0	39.0
12	37.55471122960055	39.0	37.0	39.0	35.0	39.0
13	37.546987103490515	39.0	37.0	39.0	35.0	39.0
14	38.89956451489581	41.0	37.0	41.0	35.0	41.0
15	38.958638577611374	41.0	37.0	41.0	35.0	41.0
16	38.970667141670035	41.0	37.0	41.0	35.0	41.0
17	39.012642763578555	41.0	37.0	41.0	35.0	41.0
18	39.002369415384564	41.0	37.0	41.0	35.0	41.0
19	38.92548608724658	41.0	37.0	41.0	35.0	41.0
20	38.84692316288386	41.0	36.0	41.0	35.0	41.0
21	38.80097162835131	41.0	35.0	41.0	35.0	41.0
22	38.77011293373115	41.0	35.0	41.0	35.0	41.0
23	38.75598298390058	41.0	35.0	41.0	35.0	41.0
24	38.696165328059	41.0	35.0	41.0	35.0	41.0
25	38.66487560149122	41.0	35.0	41.0	35.0	41.0
26	38.62007768657676	41.0	35.0	41.0	35.0	41.0
27	38.593991431454576	41.0	36.0	41.0	35.0	41.0
28	38.61414406549669	41.0	36.0	41.0	35.0	41.0
29	38.63500248284485	41.0	36.0	41.0	35.0	41.0
30	38.56504423328827	41.0	36.0	41.0	35.0	41.0
31	38.4961581021823	40.0	35.0	41.0	35.0	41.0
32	38.41272779366257	40.0	35.0	41.0	35.0	41.0
33	38.31938963187519	40.0	35.0	41.0	35.0	41.0
34	38.23044917225902	40.0	35.0	41.0	35.0	41.0
35	38.15792237559939	40.0	35.0	41.0	35.0	41.0
36	38.09175098956701	40.0	35.0	41.0	35.0	41.0
37	38.051907841504594	40.0	35.0	41.0	35.0	41.0
38	37.985993562247984	40.0	35.0	41.0	35.0	41.0
39	37.89961072689799	40.0	35.0	41.0	35.0	41.0
40	37.82715786944185	40.0	35.0	41.0	35.0	41.0
41	37.78755670422722	40.0	35.0	41.0	35.0	41.0
42	37.68269410931407	40.0	35.0	41.0	35.0	41.0
43	37.57085350207156	40.0	35.0	41.0	34.0	41.0
44	37.484216080600454	40.0	35.0	41.0	34.0	41.0
45	37.42892384009975	39.0	35.0	41.0	34.0	41.0
46	37.41000044531566	39.0	35.0	41.0	34.0	41.0
47	37.32784642827435	39.0	35.0	41.0	34.0	41.0
48	37.28332746580942	39.0	35.0	41.0	34.0	41.0
49	37.19384758609506	39.0	35.0	41.0	34.0	41.0
50	37.00186864532456	39.0	35.0	41.0	34.0	41.0
51	36.850428553303864	38.0	35.0	40.0	34.0	41.0
52	36.62489465764048	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	0.0
13	5.0
14	12.0
15	13.0
16	22.0
17	55.0
18	119.0
19	167.0
20	272.0
21	491.0
22	794.0
23	1281.0
24	2207.0
25	3655.0
26	5089.0
27	6097.0
28	6399.0
29	6780.0
30	7919.0
31	9844.0
32	12459.0
33	17475.0
34	47479.0
35	206659.0
36	40766.0
37	67280.0
38	128848.0
39	613625.0
40	4352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.650459977465346	22.52154529574421	25.52935848498572	19.29863624180472
2	26.800020501324607	24.042004189328054	27.051413793190367	22.106561516156976
3	25.894769389505846	24.881466214405204	26.199432516613214	23.02433187947574
4	25.486507355690417	27.68653474680444	23.414445199707266	23.412512697797872
5	23.00248620571735	35.97789217815651	21.051919604559696	19.967702011566445
6	87.94496906736617	2.4906588739227353	6.965661121506478	2.598710937204611
7	87.70710328886618	2.4778035351341448	5.249767469607206	4.565325706392464
8	77.34418783246385	5.066263810036743	11.186581378915731	6.402966978583678
9	48.19222848558228	20.831782430532858	15.824922048754503	15.151067035130364
10	36.28221921797529	20.85413223522413	27.19534317452929	15.668305372271284
11	34.67740241495521	18.019151934140336	28.306447750609788	18.996997900294666
12	20.87093659965366	29.990329088270805	29.990077022804364	19.14865728927117
13	15.241054406650495	32.80690861030427	31.5375069212976	20.414530061747637
14	13.384256159009619	23.5151873644623	43.491711667354245	19.60884480917384
15	16.21906841644912	18.59663391776112	44.502242122324006	20.68205554346575
16	16.7110161851236	21.795848817854974	29.26572489406949	32.227410102951936
17	18.91213585992554	23.26933951285828	33.33372543517002	24.484799192046157
18	23.26581059632808	27.249873337103114	28.14067269551248	21.34364337105633
19	25.734203687381687	22.577503829294546	26.653234378032664	25.035058105291107
20	20.63743995590535	29.57862215974733	27.513281749535988	22.270656134811333
21	20.05962188499597	24.42505967649918	27.17307739166016	28.342241046844684
22	18.936334144704063	28.404081107945355	24.975486633388424	27.684098113962158
23	19.000190729536275	22.66480250250595	35.76355250985786	22.571454258099912
24	19.348461182338276	22.441304455593205	33.347841101290825	24.86239326077769
25	17.340675720298076	29.196490912619826	29.532998310321158	23.929835056760943
26	19.45592509286512	27.870206450019197	27.738208167425242	24.93566028969044
27	21.00797619157648	26.136164084536034	33.85575301617336	19.000106707714124
28	19.01077747912688	24.279449858717307	30.91095619354259	25.79881646861323
29	21.921965572898593	26.890764069244067	27.930450096499065	23.25682026135828
30	27.481773566230622	25.06454976486493	23.52871487782807	23.92496179107638
31	27.900286262348057	27.05090966225748	22.67085207370058	22.377952001693878
32	27.653430148878265	25.37383409219042	25.433657629559548	21.53907812937176
33	22.02850524338181	28.596575102485616	25.83746650680115	23.537453147331426
34	20.998397703851644	25.0175815662844	29.394278281955387	24.589742447908574
35	28.10521548656617	25.28603128804613	26.697009747371585	19.91174347801611
36	21.682083270667057	27.65679102176417	29.026262700948692	21.63486300662008
37	22.32291770818717	27.805257581499067	26.70793258425078	23.163892126062983
38	20.969830284321446	26.71835129019709	27.17635424272392	25.135464182757545
39	25.855111089452155	23.013072955307955	29.061551866250703	22.07026408898919
40	18.80248738202286	21.51647625921404	31.65102040301907	28.030015955744027
41	23.685751663422025	24.594867779059577	24.73131921822736	26.988061339291043
42	23.964368025663628	22.212260968418718	28.588761073025886	25.23460993289177
43	23.543586740348204	27.331122439119888	27.884322116140005	21.240968704391904
44	18.801227054690646	28.882585385076208	27.764086888646723	24.552100671586423
45	23.23253795475761	26.73834848386823	26.44729689194878	23.581816669425386
46	24.116615567395165	23.27639734591868	28.069338168509123	24.53764891817703
47	23.13347622644553	21.284828095552978	31.326444104062706	24.255251573938782
48	20.982685623110033	20.6855004381738	29.55618833323391	28.775625605482258
49	21.205931604556337	23.95630193073745	31.112188457586203	23.72557800712001
50	20.259677843529523	25.485078984713912	28.414163726603075	25.841079445153497
51	20.730536134844943	21.57167859636505	30.801643802928496	26.89614146586151
52	21.88852488768383	20.147424689140266	33.822648418247184	24.141402004928718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	332.5
2	568.0
3	2525.0
4	4482.0
5	3015.0
6	1548.0
7	1679.5
8	1811.0
9	1837.5
10	1864.0
11	1768.5
12	1673.0
13	1623.5
14	1565.5
15	1557.0
16	1606.0
17	1655.0
18	1523.5
19	1392.0
20	1556.0
21	1720.0
22	2158.0
23	2596.0
24	2967.5
25	3339.0
26	3935.5
27	4532.0
28	6718.0
29	8904.0
30	9740.0
31	10576.0
32	12785.0
33	14994.0
34	17248.5
35	19503.0
36	22277.5
37	25052.0
38	28073.0
39	34522.0
40	37950.0
41	49086.5
42	60223.0
43	78501.5
44	96780.0
45	109561.5
46	122343.0
47	129025.0
48	135707.0
49	138550.5
50	141394.0
51	125686.0
52	109978.0
53	97710.0
54	85442.0
55	72682.0
56	59922.0
57	53393.5
58	46865.0
59	44147.0
60	41429.0
61	37692.5
62	33956.0
63	32101.0
64	24238.0
65	18230.0
66	14635.5
67	11041.0
68	9082.5
69	7124.0
70	5437.5
71	3751.0
72	3281.0
73	2811.0
74	2577.0
75	2343.0
76	1790.0
77	1237.0
78	917.0
79	597.0
80	353.0
81	109.0
82	123.5
83	138.0
84	75.5
85	13.0
86	9.0
87	5.0
88	2.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1190167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.637882131561152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.51766864782903	15.510949067789165
2	12.872866667146113	5.828288766123123
3	5.103868033348161	3.4662228886193547
4	2.5277701438875266	2.288934502920208
5	1.4484904561989156	1.639537810806115
6	0.9325847148796408	1.2667045711884524
7	0.6600089516239567	1.0458843396846864
8	0.4842647405689808	0.8770182493977301
9	0.3817708871812721	0.7778235910744052
>10	5.035653461156765	30.36452040645385
>50	1.8201117537263853	26.797775167690492
>100	0.20376607230833846	7.307586569004481
>500	0.007450313429920697	1.0488079644418273
>1k	0.0037251567149603486	1.7799461048061147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	4100	0.3444894708053576	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT	3631	0.30508323621811057	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	3578	0.30063007964428523	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.20022400217784564	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	1554	0.1305699116174453	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1500	0.1260327332214723	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.310712698302003	0.0
2	0.0	0.0	0.0	1.1890768270335172	0.0
3	0.0	0.0	0.0	1.92351157442611	0.0
4	0.0	0.0	0.0	2.3897486655234097	0.0
5	0.0	0.0	0.0	4.9247710615401035	0.0
6	0.0	0.0	0.0	5.409324909865591	0.0
7	0.0	0.0	0.0	7.735301012378935	0.0
8	0.0	0.0	0.0	10.240747726999656	0.0
9	0.0	0.0	0.0	12.511353448717701	0.0
10	0.0	0.0	0.0	13.574481564351894	0.0
11	0.0	0.0	0.0	14.637609679986086	0.0
12	8.402182214764819E-5	0.0	0.0	15.482617145324983	0.0
13	8.402182214764819E-5	0.0	0.0	15.815343561029671	0.0
14	8.402182214764819E-5	0.0	0.0	16.05581401601624	0.0
15	8.402182214764819E-5	0.0	0.0	16.943084457895406	0.0
16	8.402182214764819E-5	0.0	0.0	17.92000618400611	0.0
17	8.402182214764819E-5	0.0	0.0	19.276790568046334	0.0
18	8.402182214764819E-5	0.0	0.0	19.8799832292443	0.0
19	8.402182214764819E-5	0.0	0.0	20.675417819516085	0.0
20	8.402182214764819E-5	0.0	0.0	21.514291691838203	0.0
21	8.402182214764819E-5	0.0	0.0	22.305273125536164	0.0
22	8.402182214764819E-5	0.0	0.0	23.11499142557305	0.0
23	8.402182214764819E-5	0.0	0.0	23.70053950412001	0.0
24	2.5206546644294454E-4	0.0	0.0	24.196184232968985	0.0
25	2.5206546644294454E-4	0.0	0.0	24.70342397327434	0.0
26	2.5206546644294454E-4	0.0	0.0	25.180247813962243	0.0
27	2.5206546644294454E-4	0.0	0.0	25.66009644024746	0.0
28	2.5206546644294454E-4	0.0	0.0	26.127341793210533	0.0
29	2.5206546644294454E-4	0.0	0.0	26.74086913853266	0.0
30	2.5206546644294454E-4	0.0	0.0	27.315914489311165	0.0
31	2.5206546644294454E-4	0.0	0.0	27.81155921816014	0.0
32	3.3608728859059277E-4	0.0	0.0	28.302918834079588	0.0
33	3.3608728859059277E-4	0.0	0.0	28.759577437452055	0.0
34	3.3608728859059277E-4	0.0	0.0	29.36529075331445	0.0
35	3.3608728859059277E-4	0.0	0.0	29.85547406372383	0.0
36	3.3608728859059277E-4	0.0	0.0	30.368763375223814	0.0
37	3.3608728859059277E-4	0.0	0.0	30.850124394307688	0.0
38	3.3608728859059277E-4	0.0	0.0	31.28535743303251	0.0
39	3.3608728859059277E-4	0.0	0.0	31.812846432475443	0.0
40	3.3608728859059277E-4	0.0	0.0	32.330756944193546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	35	1.0196163E-7	46.000004	7
AAATCCG	35	1.0196163E-7	46.000004	21
TACCGAA	35	1.0196163E-7	46.000004	8
GTCGCGA	20	6.311545E-4	46.0	21
TCGTTAG	25	3.4172303E-5	46.0	12
GTTTCGA	25	3.4172303E-5	46.0	32
CGACCCA	20	6.311545E-4	46.0	35
GCGCCTA	25	3.4172303E-5	46.0	33
TTCGCGT	20	6.311545E-4	46.0	15
GCGTAAC	20	6.311545E-4	46.0	32
GTACGCA	25	3.4172303E-5	46.0	8
ATGGGCG	180	0.0	45.999996	4
CTAACGG	90	0.0	45.999996	1
CGGTCTA	110	0.0	43.909092	30
ATCGCGG	205	0.0	43.756096	1
CATGCGG	200	0.0	43.699997	1
AACGGGA	290	0.0	43.620693	3
TATCCGG	95	0.0	43.57895	1
ACGGGCC	90	0.0	43.444443	4
GCGATAG	85	0.0	43.294117	8
>>END_MODULE