FastQCFastQC Report
Thu 26 May 2016
SRR1544943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544943_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences739477
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT38440.5198268505984636Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT29280.3959555199147506Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT26270.35525107609837764Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTTG17180.23232636038713847Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.19824822137808207No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT10630.14375024510566253Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA10470.14158655374000814No Hit
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC10410.14077516947788776No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC9010.12184287002841197No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC8290.11210625888296728No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC8150.11021302893801971No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA253.4159657E-546.07
CAAACGG301.8604951E-646.01
GTTTCGA253.4159657E-546.032
CGACGGT850.046.027
CATGCGG1150.046.01
TACGGGT405.6061253E-946.03
GCGTTGT206.3099875E-446.041
CGTTCGC253.4159657E-546.041
CCATACG206.3099875E-446.034
CTCGCGG1050.046.01
GCGATAT253.4159657E-546.08
ACGTAGG800.046.01
ATACGTA301.8604951E-646.016
ATCGGGT206.3099875E-446.03
CGCCCCG206.3099875E-446.025
AGTCGAC206.3099875E-446.027
TACCGAG253.4159657E-546.016
AACACGG600.046.01
ATTACGG351.0189069E-745.9999961
CTAACGG351.0189069E-745.9999961
Produced by FastQC (version 0.11.5)