##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544943_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	739477
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93206144342555	34.0	33.0	34.0	31.0	34.0
2	33.28065646396034	34.0	33.0	34.0	31.0	34.0
3	33.400518204082076	34.0	34.0	34.0	31.0	34.0
4	36.63224549242235	37.0	37.0	37.0	35.0	37.0
5	36.62959902742073	37.0	37.0	37.0	35.0	37.0
6	36.447344542156145	37.0	37.0	37.0	35.0	37.0
7	36.281895177267174	37.0	37.0	37.0	35.0	37.0
8	36.40980855388335	37.0	37.0	37.0	35.0	37.0
9	38.278487363366274	39.0	39.0	39.0	37.0	39.0
10	38.181063102706375	39.0	39.0	39.0	35.0	39.0
11	37.703416062974235	39.0	37.0	39.0	35.0	39.0
12	37.70532957752574	39.0	37.0	39.0	35.0	39.0
13	37.709628561807875	39.0	37.0	39.0	35.0	39.0
14	39.012005782465174	41.0	38.0	41.0	35.0	41.0
15	39.03768474205418	41.0	38.0	41.0	35.0	41.0
16	39.07832562743669	41.0	38.0	41.0	35.0	41.0
17	39.14644133624169	41.0	38.0	41.0	35.0	41.0
18	39.14978694401584	41.0	38.0	41.0	35.0	41.0
19	39.10379227481044	41.0	37.0	41.0	35.0	41.0
20	39.03572524906116	41.0	37.0	41.0	35.0	41.0
21	38.995924146389946	41.0	37.0	41.0	35.0	41.0
22	38.96798412932383	41.0	37.0	41.0	35.0	41.0
23	38.928933557095085	41.0	36.0	41.0	35.0	41.0
24	38.88727979369203	41.0	36.0	41.0	35.0	41.0
25	38.854146917348345	41.0	36.0	41.0	35.0	41.0
26	38.796680626983665	41.0	36.0	41.0	35.0	41.0
27	38.868077032821844	41.0	36.0	41.0	35.0	41.0
28	38.85952909962041	41.0	36.0	41.0	35.0	41.0
29	38.89275798976844	41.0	36.0	41.0	35.0	41.0
30	38.831532285655946	41.0	36.0	41.0	35.0	41.0
31	38.76415628883657	41.0	36.0	41.0	35.0	41.0
32	38.69334407966711	41.0	36.0	41.0	35.0	41.0
33	38.61562834273412	41.0	36.0	41.0	35.0	41.0
34	38.51845561119548	41.0	36.0	41.0	35.0	41.0
35	38.458188692819384	41.0	36.0	41.0	35.0	41.0
36	38.396054238333306	40.0	36.0	41.0	35.0	41.0
37	38.37145712442713	40.0	35.0	41.0	35.0	41.0
38	38.295192413016224	40.0	35.0	41.0	35.0	41.0
39	38.23612904796228	40.0	35.0	41.0	35.0	41.0
40	38.1769426229619	40.0	35.0	41.0	35.0	41.0
41	38.156883851695184	40.0	35.0	41.0	35.0	41.0
42	38.08049878495207	40.0	35.0	41.0	35.0	41.0
43	37.99985124621861	40.0	35.0	41.0	35.0	41.0
44	37.85589680274032	40.0	35.0	41.0	35.0	41.0
45	37.845807239440845	40.0	35.0	41.0	35.0	41.0
46	37.75982755379816	40.0	35.0	41.0	35.0	41.0
47	37.649064135869	39.0	35.0	41.0	35.0	41.0
48	37.54576951007266	39.0	35.0	41.0	35.0	41.0
49	37.45641581820665	39.0	35.0	41.0	35.0	41.0
50	37.23543260980396	39.0	35.0	41.0	35.0	41.0
51	37.05830877769018	39.0	35.0	40.0	35.0	41.0
52	36.60166171496882	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	4.0
15	9.0
16	18.0
17	26.0
18	51.0
19	86.0
20	149.0
21	233.0
22	381.0
23	645.0
24	1097.0
25	1948.0
26	2755.0
27	3299.0
28	3237.0
29	3217.0
30	3688.0
31	4366.0
32	5617.0
33	8463.0
34	26639.0
35	121313.0
36	23044.0
37	40977.0
38	84295.0
39	402535.0
40	1382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07604834227434	22.244775699582274	24.51827440204361	21.160901556099784
2	26.500080462272663	24.391968918573532	26.952427188404776	22.15552343074903
3	25.82243937269178	24.96047882489922	26.072886648266273	23.144195154142725
4	25.530611499749146	27.392197458474026	23.70256275719191	23.374628284584915
5	23.00869398236862	35.63491494664473	21.14710802364374	20.209283047342918
6	86.53737709218812	2.4813483042745075	7.756022161608812	3.225252441928552
7	86.27056690066087	2.3146088384087675	6.022229224167892	5.392595036762469
8	75.34919950181006	4.995151979033831	12.360357387721322	7.295291131434785
9	47.11884210056567	20.16114091445711	16.477862056561595	16.242154928415623
10	35.040575974641534	20.38089081878138	28.51163727877946	16.06689592779762
11	33.452696973671934	17.76485272699489	28.71488903644062	20.067561262892557
12	20.434036487950266	28.466064529390366	31.18988149732852	19.91001748533085
13	14.99411070256411	31.448442615524215	32.262125799720614	21.295320882191064
14	13.318737431995856	23.075903645414257	42.979159595227436	20.626199327362446
15	15.952220285417937	18.435867511768453	44.16513292502674	21.446779277786867
16	16.402809012315462	22.313337669731446	29.726549980594392	31.5573033373587
17	18.469269497225742	23.16218083861973	33.52463971157994	24.843909952574588
18	22.614902153819525	27.685242407809845	28.32258474570541	21.37727069266522
19	24.79225182121959	22.606247388356906	26.910911360326285	25.690589430097216
20	19.956401618982063	30.1684839420293	27.318361490621072	22.55675294836756
21	19.689320966034103	24.393321225677067	27.052092221935233	28.8652655863536
22	18.739866148642893	28.80400607456351	24.814294427007198	27.641833349786403
23	18.53999515874057	22.815449297273613	35.39366335937426	23.25089218461156
24	19.652402982107624	22.64343583370409	32.59560473145209	25.10855645273619
25	17.39364442707481	28.67080382486541	29.672728157873742	24.262823590186038
26	19.74490078798935	26.826527397065764	27.532026012979443	25.89654580196544
27	21.79783820186429	25.463672298124212	33.51463263901379	19.223856860997703
28	19.435628153411127	24.248759596309284	30.333059716529387	25.9825525337502
29	22.535792188262786	26.27154056177542	27.3751583889695	23.817508860992294
30	27.577328300947833	24.59833098257282	23.015996440727704	24.808344275751644
31	28.300406909207453	26.70184468211993	21.91400138205786	23.083747026614756
32	27.803434048658715	24.466616270688608	25.48301028970475	22.246939390947926
33	22.659257826815438	28.034543332652675	25.667194517206077	23.63900432332581
34	21.753347298158022	24.436595052990153	29.42525595792702	24.3848016909248
35	27.85265802722735	25.611614695250832	26.634499788363936	19.901227489157876
36	21.848549718246815	27.24858244407872	28.836055752917268	22.0668120847572
37	22.61760676802659	27.637370736344742	26.74849927719185	22.996523218436813
38	21.09193389381955	26.205547975122954	26.74349574090878	25.95902239014871
39	26.408394040653054	22.316853668200633	29.21510743403784	22.05964485710847
40	19.156917659372773	20.679480227241687	31.751224176005472	28.412377937380068
41	23.809124556950383	24.398189531249788	24.626864662457386	27.165821249342443
42	23.77991472351405	21.499924946955755	28.935856017158073	25.784304312372125
43	24.31380556798927	26.574998275808444	27.78815297838878	21.32304317781351
44	18.97300389329215	27.49010449276989	28.64808506552604	24.888806548411917
45	23.224522196092646	26.060310192203406	26.571076585208193	24.144091026495754
46	24.467698116371437	21.957545670791653	28.55274741472689	25.02200879811002
47	23.01870105493477	20.29339654918273	31.811672303533445	24.876230092349054
48	20.829045392892546	19.833476903270824	29.368864751709655	29.96861295212698
49	21.165634630962153	22.645193832938684	31.607879623030872	24.58129191306829
50	20.730462205044915	24.065792445201133	29.007663524355724	26.19608182539822
51	20.65892515926797	20.56196473994458	31.109013532537183	27.670096568250262
52	21.5575332295663	19.451585377232828	34.55009418818976	24.44078720501111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	222.5
2	380.0
3	1592.0
4	2804.0
5	1942.0
6	1080.0
7	1104.5
8	1129.0
9	1146.0
10	1163.0
11	1128.5
12	1094.0
13	1059.0
14	1001.5
15	979.0
16	966.0
17	953.0
18	927.0
19	901.0
20	919.0
21	937.0
22	1238.5
23	1540.0
24	1673.0
25	1806.0
26	2146.0
27	2486.0
28	3552.0
29	4618.0
30	5226.0
31	5834.0
32	6968.0
33	8102.0
34	9395.0
35	10688.0
36	12184.5
37	13681.0
38	15605.0
39	19312.5
40	21096.0
41	27811.5
42	34527.0
43	45360.5
44	56194.0
45	64502.5
46	72811.0
47	80667.0
48	88523.0
49	91715.5
50	94908.0
51	84432.0
52	73956.0
53	64756.5
54	55557.0
55	47007.0
56	38457.0
57	33699.5
58	28942.0
59	27202.0
60	25462.0
61	23236.0
62	21010.0
63	20110.0
64	15279.0
65	11348.0
66	9092.5
67	6837.0
68	5789.5
69	4742.0
70	3609.0
71	2476.0
72	2161.0
73	1846.0
74	1657.0
75	1468.0
76	1114.5
77	761.0
78	563.0
79	365.0
80	219.0
81	73.0
82	89.5
83	106.0
84	56.0
85	6.0
86	3.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	739477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.563671803961988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31816910542211	19.382269350773544
2	11.999094962496166	6.614782309816358
3	4.437351525717385	3.6692910340105196
4	2.11047503792427	2.3268976518319526
5	1.2169244223815356	1.677145269437533
6	0.8004011868449453	1.323719737541744
7	0.6011556922966816	1.1599040743884506
8	0.4528007679217214	0.998468140766103
9	0.4355497221408662	1.0804814635857907
>10	7.175771535025158	47.952308271237214
>50	0.3455047240094891	6.308763655451198
>100	0.09991091021840937	4.783802098328406
>500	0.002953031829115548	0.5876820091869285
>1k	0.003937375772154064	2.134484933644306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	3844	0.5198268505984636	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT	2928	0.3959555199147506	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	2627	0.35525107609837764	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTTG	1718	0.23232636038713847	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1466	0.19824822137808207	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	1063	0.14375024510566253	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1047	0.14158655374000814	No Hit
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1041	0.14077516947788776	No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	901	0.12184287002841197	No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	829	0.11210625888296728	No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	815	0.11021302893801971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40393413182560106	0.0
2	0.0	0.0	0.0	1.5313525640418837	0.0
3	0.0	0.0	0.0	2.4154909483324025	0.0
4	0.0	0.0	0.0	2.967908400126035	0.0
5	1.352307103533984E-4	0.0	0.0	5.869688982889258	0.0
6	1.352307103533984E-4	0.0	0.0	6.428867970200561	0.0
7	1.352307103533984E-4	0.0	0.0	9.186763077147768	0.0
8	1.352307103533984E-4	0.0	0.0	12.056223520136529	0.0
9	1.352307103533984E-4	0.0	0.0	14.876595215267006	0.0
10	2.704614207067968E-4	0.0	0.0	16.16480296209348	0.0
11	2.704614207067968E-4	0.0	0.0	17.403381038220257	0.0
12	2.704614207067968E-4	0.0	0.0	18.43181059045785	0.0
13	2.704614207067968E-4	0.0	0.0	18.818435191358216	0.0
14	2.704614207067968E-4	0.0	0.0	19.105665220148836	0.0
15	2.704614207067968E-4	0.0	0.0	20.13720507872456	0.0
16	4.056921310601952E-4	0.0	0.0	21.245150288649953	0.0
17	4.056921310601952E-4	0.0	0.0	22.681841355444455	0.0
18	4.056921310601952E-4	0.0	0.0	23.340414914865505	0.0
19	4.056921310601952E-4	0.0	0.0	24.17898054976693	0.0
20	4.056921310601952E-4	0.0	0.0	25.080158003561976	0.0
21	4.056921310601952E-4	0.0	0.0	25.948609625451503	0.0
22	4.056921310601952E-4	0.0	0.0	26.8180078623135	0.0
23	4.056921310601952E-4	0.0	0.0	27.470360809058295	0.0
24	4.056921310601952E-4	0.0	0.0	28.002358423588564	0.0
25	4.056921310601952E-4	0.0	0.0	28.553964491120077	0.0
26	4.056921310601952E-4	0.0	0.0	29.077172109477374	0.0
27	4.056921310601952E-4	0.0	0.0	29.58996696313746	0.0
28	4.056921310601952E-4	0.0	0.0	30.068818908498844	0.0
29	4.056921310601952E-4	0.0	0.0	30.67113649241288	0.0
30	4.056921310601952E-4	0.0	0.0	31.257902544636277	0.0
31	4.056921310601952E-4	0.0	0.0	31.7801635480211	0.0
32	4.056921310601952E-4	0.0	0.0	32.27997625348726	0.0
33	4.056921310601952E-4	0.0	0.0	32.74408805142013	0.0
34	4.056921310601952E-4	0.0	0.0	33.37466885379802	0.0
35	4.056921310601952E-4	0.0	0.0	33.85852433544248	0.0
36	4.056921310601952E-4	0.0	0.0	34.35860750232935	0.0
37	4.056921310601952E-4	0.0	0.0	34.845978982443	0.0
38	4.056921310601952E-4	0.0	0.0	35.29629724791981	0.0
39	4.056921310601952E-4	0.0	0.0	35.8111205622352	0.0
40	5.409228414135936E-4	0.0	0.0	36.33960217829628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	25	3.4159657E-5	46.0	7
CAAACGG	30	1.8604951E-6	46.0	1
GTTTCGA	25	3.4159657E-5	46.0	32
CGACGGT	85	0.0	46.0	27
CATGCGG	115	0.0	46.0	1
TACGGGT	40	5.6061253E-9	46.0	3
GCGTTGT	20	6.3099875E-4	46.0	41
CGTTCGC	25	3.4159657E-5	46.0	41
CCATACG	20	6.3099875E-4	46.0	34
CTCGCGG	105	0.0	46.0	1
GCGATAT	25	3.4159657E-5	46.0	8
ACGTAGG	80	0.0	46.0	1
ATACGTA	30	1.8604951E-6	46.0	16
ATCGGGT	20	6.3099875E-4	46.0	3
CGCCCCG	20	6.3099875E-4	46.0	25
AGTCGAC	20	6.3099875E-4	46.0	27
TACCGAG	25	3.4159657E-5	46.0	16
AACACGG	60	0.0	46.0	1
ATTACGG	35	1.0189069E-7	45.999996	1
CTAACGG	35	1.0189069E-7	45.999996	1
>>END_MODULE