FastQCFastQC Report
Thu 26 May 2016
SRR1544942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544942_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences726462
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT19820.27282913627966776Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT16360.225201042862531Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT14820.20400241168842967Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.19849627372113063No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT12100.16656067351079615No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10150.1397182509202133No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG9620.13242261811354206No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA8380.11535359041491502No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA7540.10379070068358702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT301.8604496E-646.016
TCGATGG405.6061253E-946.01
CTAACGG750.046.01
CACTAGG600.046.01
TTGCGTT206.3099124E-446.028
GCGATAC301.8604496E-646.08
CACCGTA206.3099124E-446.023
ATACCGG453.092282E-1046.01
CGGGATA1200.044.0833325
ACGGGCG1150.044.04
CCCGCAC3650.042.84931632
GCTTATG4050.042.59259424
ATCAACG4250.042.21176514
ACGGGAT2350.042.0851064
CACTTAC3450.042.036
CATACGA2250.041.9111117
ACATACG2250.041.9111116
AGGGATT3250.041.753855
AACGCAA4250.041.67058617
CGCAAGC4250.041.67058619
Produced by FastQC (version 0.11.5)