SRR1544941_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544941_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	450142
Sequences flagged as poor quality	0
Sequence length	52
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	1637	0.36366302189086996	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1534	0.34078135343958127	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT	1454	0.32300918376867743	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	1105	0.24547809357935937	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.20726792878691613	No Hit
TGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTTG	802	0.17816600095081106	Illumina Single End Adapter 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	593	0.13173620768557479	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	520	0.11551910286087502	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	451	0.10019060651972045	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	70	0.0	46.000004	1
CTAGACG	25	3.4138207E-5	46.0	25
ACCGCTA	25	3.4138207E-5	46.0	21
CCTACGG	20	6.307344E-4	46.0	1
TACGGGA	65	0.0	46.0	3
GCCGATG	20	6.307344E-4	46.0	8
TCTACCG	20	6.307344E-4	46.0	35
CTCGCGG	40	5.5988494E-9	46.0	1
CTCGAGG	45	3.092282E-10	46.0	1
AGTCGGC	20	6.307344E-4	46.0	46
CGAAGTT	20	6.307344E-4	46.0	14
ATCAACG	260	0.0	43.346153	14
CGCACTT	235	0.0	43.06383	34
GCACTTA	230	0.0	43.0	35
TACACGG	65	0.0	42.46154	1
CTTATGA	245	0.0	42.2449	25
TTGACGG	60	1.8189894E-12	42.166668	1
GTCATAC	230	0.0	42.0	38
CGTCATA	230	0.0	42.0	37
ACGGGTA	55	4.7293724E-11	41.81818	4