FastQCFastQC Report
Thu 26 May 2016
SRR1544941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544941_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences450142
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT16370.36366302189086996Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA15340.34078135343958127No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT14540.32300918376867743Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT11050.24547809357935937Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.20726792878691613No Hit
TGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTTG8020.17816600095081106Illumina Single End Adapter 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5930.13173620768557479No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT5200.11551910286087502No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG4510.10019060651972045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG700.046.0000041
CTAGACG253.4138207E-546.025
ACCGCTA253.4138207E-546.021
CCTACGG206.307344E-446.01
TACGGGA650.046.03
GCCGATG206.307344E-446.08
TCTACCG206.307344E-446.035
CTCGCGG405.5988494E-946.01
CTCGAGG453.092282E-1046.01
AGTCGGC206.307344E-446.046
CGAAGTT206.307344E-446.014
ATCAACG2600.043.34615314
CGCACTT2350.043.0638334
GCACTTA2300.043.035
TACACGG650.042.461541
CTTATGA2450.042.244925
TTGACGG601.8189894E-1242.1666681
GTCATAC2300.042.038
CGTCATA2300.042.037
ACGGGTA554.7293724E-1141.818184