##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544941_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	450142
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.888353008606174	34.0	33.0	34.0	31.0	34.0
2	33.26859524327879	34.0	33.0	34.0	31.0	34.0
3	33.402706257136636	34.0	34.0	34.0	31.0	34.0
4	36.61631662897486	37.0	37.0	37.0	35.0	37.0
5	36.62296342043177	37.0	37.0	37.0	35.0	37.0
6	36.423099821834	37.0	37.0	37.0	35.0	37.0
7	36.21920194072093	37.0	37.0	37.0	35.0	37.0
8	36.350793749527924	37.0	37.0	37.0	35.0	37.0
9	38.23045616716503	39.0	39.0	39.0	37.0	39.0
10	38.16222436475601	39.0	39.0	39.0	35.0	39.0
11	37.715205424066184	39.0	37.0	39.0	35.0	39.0
12	37.71010703289184	39.0	37.0	39.0	35.0	39.0
13	37.71930857373896	39.0	37.0	39.0	35.0	39.0
14	39.026878185106035	41.0	38.0	41.0	35.0	41.0
15	39.04287980237347	41.0	38.0	41.0	35.0	41.0
16	39.06261801831422	41.0	38.0	41.0	35.0	41.0
17	39.131838397661184	41.0	38.0	41.0	35.0	41.0
18	39.12830173589667	41.0	38.0	41.0	35.0	41.0
19	39.08402015364041	41.0	37.0	41.0	35.0	41.0
20	39.02339928289295	41.0	37.0	41.0	35.0	41.0
21	38.96614623829814	41.0	37.0	41.0	35.0	41.0
22	38.937892931563816	41.0	37.0	41.0	35.0	41.0
23	38.89357802648942	41.0	36.0	41.0	35.0	41.0
24	38.83663377334263	41.0	36.0	41.0	35.0	41.0
25	38.78409035371061	41.0	36.0	41.0	35.0	41.0
26	38.711728743374316	41.0	36.0	41.0	35.0	41.0
27	38.76871742694527	41.0	36.0	41.0	35.0	41.0
28	38.778352164428114	41.0	36.0	41.0	35.0	41.0
29	38.79246771018923	41.0	36.0	41.0	35.0	41.0
30	38.74818612793296	41.0	36.0	41.0	35.0	41.0
31	38.689624607346126	41.0	36.0	41.0	35.0	41.0
32	38.623292205570685	41.0	36.0	41.0	35.0	41.0
33	38.55008419565382	41.0	36.0	41.0	35.0	41.0
34	38.42293098622213	40.0	36.0	41.0	35.0	41.0
35	38.33614503867668	40.0	35.0	41.0	35.0	41.0
36	38.284772360721725	40.0	35.0	41.0	35.0	41.0
37	38.214227954734284	40.0	35.0	41.0	35.0	41.0
38	38.15031034651288	40.0	35.0	41.0	35.0	41.0
39	38.061676093321665	40.0	35.0	41.0	35.0	41.0
40	37.98017958777452	40.0	35.0	41.0	35.0	41.0
41	37.92733404125809	40.0	35.0	41.0	35.0	41.0
42	37.858349143159266	40.0	35.0	41.0	35.0	41.0
43	37.794324901919836	40.0	35.0	41.0	35.0	41.0
44	37.657876847750266	40.0	35.0	41.0	35.0	41.0
45	37.658261170919396	40.0	35.0	41.0	35.0	41.0
46	37.60180343091736	40.0	35.0	41.0	35.0	41.0
47	37.507566501237385	39.0	35.0	41.0	35.0	41.0
48	37.42263996694376	39.0	35.0	41.0	35.0	41.0
49	37.357360566221324	39.0	35.0	41.0	35.0	41.0
50	37.29846359593195	39.0	35.0	41.0	35.0	41.0
51	37.214538967703525	39.0	35.0	41.0	34.0	41.0
52	36.71184870551959	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	7.0
15	4.0
16	10.0
17	19.0
18	36.0
19	54.0
20	100.0
21	184.0
22	264.0
23	418.0
24	735.0
25	1196.0
26	1673.0
27	2105.0
28	2101.0
29	2115.0
30	2333.0
31	2811.0
32	3660.0
33	5466.0
34	19116.0
35	70111.0
36	17583.0
37	29454.0
38	52897.0
39	234225.0
40	1462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.72002612508941	21.915973181795966	23.037619240150885	21.32638145296373
2	27.84121455007531	24.09417472708612	25.49240017594448	22.57221054689409
3	28.116683179974316	25.080752295942172	24.452061793833945	22.350502730249566
4	26.75489067894131	25.900049317770836	22.33761790723816	25.007442096049694
5	25.146953627966283	35.84024596682824	19.93437626349019	19.07842414171528
6	86.58912076633595	3.3940401029008624	6.824513153627078	3.192325977136104
7	85.79137250023327	3.252751354017177	5.897694505289442	5.058181640460122
8	74.3716427260731	6.43907922388935	11.321316384607524	7.867961665430021
9	47.513673463040554	20.87385758271834	16.519453861226012	15.093015093015094
10	35.904892234006155	22.192774724420296	25.882499300220818	16.01983374135273
11	34.821678492564565	18.328660733724025	26.86196800120851	19.9876927725029
12	21.191312963464863	28.327505542695413	29.63731444744103	20.843867046398692
13	16.379275872946756	31.211928680283112	29.290757138858403	23.118038307911725
14	14.955947234428246	24.357202838215496	39.66015168546814	21.026698241888113
15	17.204348849918468	19.199497047598314	41.50356998458265	22.092584117900575
16	18.420409559650068	21.494106304232886	28.895326363680795	31.190157772436255
17	20.023903568207366	23.32774991002839	31.533827103447354	25.11451941831689
18	23.776497194218713	27.06234921424795	26.75444637469954	22.406707216833798
19	26.421440345490975	23.100710442482594	26.12309004714068	24.35475916488575
20	22.78880886475823	28.629188122859006	26.69913049659885	21.88287251578391
21	21.29172572210547	24.301664807993923	25.119406764976386	29.287202704924226
22	20.507084431135063	28.762701547511675	23.96577080121384	26.764443220139423
23	20.449991336067285	22.36405400962363	33.67781722212102	23.508137432188065
24	20.006575702778235	23.281542268884042	31.539603058590398	25.172278969747325
25	19.944150956809185	27.504654086932568	28.3423897347948	24.20880522146345
26	20.71257514295489	26.628708274277894	27.324710869014666	25.33400571375255
27	23.12314780669211	24.993446512433852	32.12430744076314	19.759098240110898
28	20.770112542264442	24.62089740570753	28.734488228159115	25.87450182386891
29	22.034380262228364	25.61058510425599	28.20021237742757	24.154822256088078
30	26.496749914471433	24.596904976651814	22.88322351613491	26.023121592741845
31	28.096023032731892	24.04130252231518	25.132513740108674	22.73016070484425
32	26.11820270048118	25.76542513251374	25.47596091899889	22.640411248006185
33	24.435400384767473	23.19179281204598	26.36079281648902	26.01201398669753
34	25.97380382190509	22.924321658498872	28.185994641690844	22.915879877905194
35	30.086728187994012	22.948314087554593	24.00709109569869	22.957866628752704
36	24.84371598295649	27.06168275788529	25.553269857067324	22.541331402090893
37	25.019216158456665	25.25669677568412	26.712681775972914	23.0114052898863
38	25.085195338359895	24.326767997654073	25.48973435049384	25.098302313492187
39	29.14702471664497	21.336378298403616	27.22807469642913	22.288522288522287
40	21.19975474405854	20.15874990558535	30.631223036286325	28.010272314069784
41	24.00309235752274	24.97034269186168	25.10829915893207	25.918265791683513
42	24.993890816675627	22.231651345575397	27.11388850629357	25.660569331455406
43	25.139178303735264	25.751873853139678	26.482087874492937	22.62685996863212
44	20.895184186323426	26.855525589702804	27.389357136192576	24.85993308778119
45	23.587445739344474	25.02277059239085	26.320583282608602	25.06920038565608
46	25.32245380346646	22.37782744111858	28.18688325017439	24.112835505240568
47	25.157172625527057	20.605275668566808	29.70018349765185	24.537368208254286
48	22.612642232895396	20.155639775892944	28.13423319752434	29.09748479368732
49	21.908420009685834	23.543681771529872	30.063624367421838	24.48427385136246
50	21.787124951681914	24.267453381377432	27.399353981632462	26.546067685308188
51	22.095027791230322	21.695820429997646	29.90522990522991	26.303921873542123
52	23.889350471628955	20.105655548693523	31.328114239506643	24.67687974017088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	173.5
2	280.0
3	1037.5
4	1795.0
5	1241.0
6	687.0
7	675.5
8	664.0
9	668.0
10	672.0
11	666.0
12	660.0
13	654.0
14	630.5
15	613.0
16	619.5
17	626.0
18	591.5
19	557.0
20	586.5
21	616.0
22	747.0
23	878.0
24	1088.5
25	1299.0
26	1638.0
27	1977.0
28	2868.5
29	3760.0
30	4229.5
31	4699.0
32	5089.5
33	5480.0
34	6133.5
35	6787.0
36	7341.5
37	7896.0
38	9301.5
39	12013.0
40	13319.0
41	16615.5
42	19912.0
43	23512.5
44	27113.0
45	30087.5
46	33062.0
47	34007.5
48	34953.0
49	39774.5
50	44596.0
51	46334.5
52	48073.0
53	43168.0
54	38263.0
55	32985.5
56	27708.0
57	24495.5
58	21283.0
59	19981.0
60	18679.0
61	18706.5
62	18734.0
63	18077.5
64	14410.5
65	11400.0
66	9626.0
67	7852.0
68	6648.0
69	5444.0
70	4708.5
71	3973.0
72	3240.5
73	2508.0
74	2339.5
75	2171.0
76	1740.0
77	1309.0
78	977.0
79	645.0
80	434.5
81	224.0
82	157.5
83	91.0
84	55.5
85	20.0
86	18.5
87	17.0
88	9.5
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	450142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.809323379104846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.30515433633143	21.255584042086834
2	8.980381191596033	5.353981740158341
3	3.095607057019088	2.7683385545196315
4	1.7503236706186962	2.08703857262298
5	1.2964395343004376	1.9322992659708917
6	1.0648989833888967	1.904635089715177
7	0.9306060952690929	1.941851662270971
8	0.9175803232509758	2.1881958865673514
9	0.8456381455472534	2.268711084809214
>10	9.622551404993493	50.68365446116668
>50	0.13394763578647415	2.674566022815448
>100	0.050885135382571195	3.0276801190720666
>500	0.0029932432577983053	0.635293080315129
>1k	0.0029932432577983053	1.278170417909301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	1637	0.36366302189086996	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1534	0.34078135343958127	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT	1454	0.32300918376867743	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	1105	0.24547809357935937	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.20726792878691613	No Hit
TGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTTG	802	0.17816600095081106	Illumina Single End Adapter 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	593	0.13173620768557479	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	520	0.11551910286087502	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	451	0.10019060651972045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3292294431534938	0.0
2	0.0	0.0	0.0	1.1012080632333796	0.0
3	0.0	0.0	0.0	1.7187909592972883	0.0
4	0.0	0.0	0.0	2.126217949002759	0.0
5	0.0	0.0	0.0	4.220223840477005	0.0
6	0.0	0.0	0.0	4.656974910139467	0.0
7	0.0	0.0	0.0	6.732320023459264	0.0
8	0.0	0.0	0.0	8.916741828134233	0.0
9	0.0	0.0	0.0	11.197133349032082	0.0
10	0.0	0.0	0.0	12.119286802831107	0.0
11	0.0	0.0	0.0	12.978571206419307	0.0
12	0.0	0.0	0.0	13.720559290179544	0.0
13	0.0	0.0	0.0	14.01846528428807	0.0
14	0.0	0.0	0.0	14.237951579723731	0.0
15	0.0	0.0	0.0	14.971942187132061	0.0
16	0.0	0.0	0.0	15.751696131442968	0.0
17	0.0	0.0	0.0	16.738495852419902	0.0
18	0.0	0.0	0.0	17.21279063051215	0.0
19	0.0	0.0	0.0	17.803715272069702	0.0
20	4.443042417725962E-4	0.0	0.0	18.453954529903896	0.0
21	4.443042417725962E-4	0.0	0.0	19.06176273264881	0.0
22	4.443042417725962E-4	0.0	0.0	19.702227297164008	0.0
23	4.443042417725962E-4	0.0	0.0	20.15919420982712	0.0
24	4.443042417725962E-4	0.0	0.0	20.577728805576907	0.0
25	4.443042417725962E-4	0.0	0.0	20.975825406205153	0.0
26	4.443042417725962E-4	0.0	0.0	21.345264383239066	0.0
27	4.443042417725962E-4	0.0	0.0	21.722700836624888	0.0
28	4.443042417725962E-4	0.0	0.0	22.088363227603732	0.0
29	4.443042417725962E-4	0.0	0.0	22.556437746311165	0.0
30	4.443042417725962E-4	0.0	0.0	23.0000755317211	0.0
31	4.443042417725962E-4	0.0	0.0	23.409724042635435	0.0
32	4.443042417725962E-4	0.0	0.0	23.78560543117505	0.0
33	4.443042417725962E-4	0.0	0.0	24.13305134824122	0.0
34	4.443042417725962E-4	0.0	0.0	24.585353066365723	0.0
35	4.443042417725962E-4	0.0	0.0	24.965233193081293	0.0
36	4.443042417725962E-4	0.0	0.0	25.37776968156715	0.0
37	6.664563626588943E-4	0.0	0.0	25.745209289513088	0.0
38	6.664563626588943E-4	0.0	0.0	26.089322924765963	0.0
39	6.664563626588943E-4	0.0	0.0	26.480532809646732	0.0
40	6.664563626588943E-4	0.0	0.0	26.86352306605471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	70	0.0	46.000004	1
CTAGACG	25	3.4138207E-5	46.0	25
ACCGCTA	25	3.4138207E-5	46.0	21
CCTACGG	20	6.307344E-4	46.0	1
TACGGGA	65	0.0	46.0	3
GCCGATG	20	6.307344E-4	46.0	8
TCTACCG	20	6.307344E-4	46.0	35
CTCGCGG	40	5.5988494E-9	46.0	1
CTCGAGG	45	3.092282E-10	46.0	1
AGTCGGC	20	6.307344E-4	46.0	46
CGAAGTT	20	6.307344E-4	46.0	14
ATCAACG	260	0.0	43.346153	14
CGCACTT	235	0.0	43.06383	34
GCACTTA	230	0.0	43.0	35
TACACGG	65	0.0	42.46154	1
CTTATGA	245	0.0	42.2449	25
TTGACGG	60	1.8189894E-12	42.166668	1
GTCATAC	230	0.0	42.0	38
CGTCATA	230	0.0	42.0	37
ACGGGTA	55	4.7293724E-11	41.81818	4
>>END_MODULE