FastQCFastQC Report
Thu 26 May 2016
SRR1544940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544940_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences852510
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT30450.3571805609318366TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT30270.3550691487489883TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT25670.3011108374095319TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.21043741422388007No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC10850.12727123435502222TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT9780.11472006193475735TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC9280.10885502809351211TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC8590.10076128139259363TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG351.0191616E-746.0000045
TACGCGG1100.046.0000041
GAAACGG551.8189894E-1246.0000041
TCACGCG206.310533E-446.012
ATGGGCG453.092282E-1046.04
CGTGGAT253.4164084E-546.026
CCGATAT301.8608334E-646.011
ACGCAAC206.310533E-446.036
GGATCGC253.4164084E-546.07
TGTATCG206.310533E-446.045
ACGGGCG600.046.04
CTAACGG253.4164084E-546.01
TACGCAA206.310533E-446.035
ACCCGCA650.046.031
TTACCGG405.6079443E-946.01
TCGACTC206.310533E-446.036
CCCGCAC650.046.032
GTACGCA206.310533E-446.034
CATGCGG2000.044.851
ACATACG950.043.57894516
Produced by FastQC (version 0.11.5)