FastQCFastQC Report
Thu 26 May 2016
SRR1544939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544939_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences490457
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT24920.5080975498361732TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT23190.47282432506825267TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT17050.3476349608630318TruSeq Adapter, Index 15 (95% over 22bp)
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG13340.27199122451101726TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.22631953463810284No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC6650.13558782931021476TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT6340.12926719365815964TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC6270.12783995334963105TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC5750.11723759677199021TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC5480.11173252701052283TruSeq Adapter, Index 14 (95% over 21bp)
CTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTGAAA5180.10561578283111465TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG351.01796104E-746.0000041
ATGGGCG253.4142708E-546.04
CGGCGCT253.4142708E-546.016
GGTCGGC206.307898E-446.02
CATGCGG650.046.01
ATTACGG501.6370905E-1146.01
GGATCGA253.4142708E-546.07
CCAGCGG600.046.01
AGGGTCG206.307898E-446.05
CACGAGG501.6370905E-1146.01
TACGGGT206.307898E-446.03
TGCGGGT405.6006684E-946.03
ACGGGTA301.8592054E-646.04
TCGGCGT206.307898E-446.04
AGTGCGG453.092282E-1046.01
CTAACGC206.307898E-446.044
ACGGCGC253.4142708E-546.015
CTACGTC206.307898E-446.027
GGAGTAA253.4142708E-546.07
CTCGCGG551.8189894E-1246.01
Produced by FastQC (version 0.11.5)