##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544939_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	490457
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95902189182742	34.0	33.0	34.0	31.0	34.0
2	33.30175326277329	34.0	34.0	34.0	31.0	34.0
3	33.41727205443087	34.0	34.0	34.0	31.0	34.0
4	36.64693540922038	37.0	37.0	37.0	35.0	37.0
5	36.637491156207375	37.0	37.0	37.0	35.0	37.0
6	36.4617897185686	37.0	37.0	37.0	35.0	37.0
7	36.303361966492474	37.0	37.0	37.0	35.0	37.0
8	36.42130910558928	37.0	37.0	37.0	35.0	37.0
9	38.28280970604966	39.0	39.0	39.0	37.0	39.0
10	38.165229571603625	39.0	39.0	39.0	35.0	39.0
11	37.70830266465766	39.0	37.0	39.0	35.0	39.0
12	37.71839529255368	39.0	37.0	39.0	35.0	39.0
13	37.716592891935484	39.0	37.0	39.0	35.0	39.0
14	39.00336217038395	41.0	38.0	41.0	35.0	41.0
15	39.021088495015874	41.0	38.0	41.0	35.0	41.0
16	39.04458698723843	41.0	38.0	41.0	35.0	41.0
17	39.12994207443262	41.0	38.0	41.0	35.0	41.0
18	39.13508421737278	41.0	38.0	41.0	35.0	41.0
19	39.11548413010723	41.0	38.0	41.0	35.0	41.0
20	39.04525167343926	41.0	37.0	41.0	35.0	41.0
21	39.0070791119303	41.0	37.0	41.0	35.0	41.0
22	38.98423307242021	41.0	37.0	41.0	35.0	41.0
23	38.95661393353546	41.0	37.0	41.0	35.0	41.0
24	38.9369669512312	41.0	37.0	41.0	35.0	41.0
25	38.89772395949084	41.0	37.0	41.0	35.0	41.0
26	38.845558326214125	41.0	36.0	41.0	35.0	41.0
27	38.91760745590337	41.0	36.0	41.0	35.0	41.0
28	38.93968278564686	41.0	37.0	41.0	35.0	41.0
29	38.94718191401081	41.0	37.0	41.0	35.0	41.0
30	38.90018900739514	41.0	37.0	41.0	35.0	41.0
31	38.856199422171564	41.0	37.0	41.0	35.0	41.0
32	38.80386659788728	41.0	36.0	41.0	35.0	41.0
33	38.751333959959794	41.0	36.0	41.0	35.0	41.0
34	38.6454347679817	41.0	36.0	41.0	35.0	41.0
35	38.584966673938794	41.0	36.0	41.0	35.0	41.0
36	38.55895623877323	41.0	36.0	41.0	35.0	41.0
37	38.50011315976732	41.0	36.0	41.0	35.0	41.0
38	38.4782743441321	41.0	36.0	41.0	35.0	41.0
39	38.42588443023548	40.0	36.0	41.0	35.0	41.0
40	38.36979184719557	40.0	36.0	41.0	35.0	41.0
41	38.35383937837568	40.0	36.0	41.0	35.0	41.0
42	38.291513833016964	40.0	35.0	41.0	35.0	41.0
43	38.22576087200305	40.0	35.0	41.0	35.0	41.0
44	38.09226293028747	40.0	35.0	41.0	35.0	41.0
45	38.10738759972842	40.0	35.0	41.0	35.0	41.0
46	38.059570971563254	40.0	35.0	41.0	35.0	41.0
47	37.98268145831337	40.0	35.0	41.0	35.0	41.0
48	37.947803783002385	40.0	35.0	41.0	35.0	41.0
49	37.90725384692236	40.0	35.0	41.0	35.0	41.0
50	37.82997082313027	40.0	35.0	41.0	35.0	41.0
51	37.766846023198774	40.0	35.0	41.0	35.0	41.0
52	37.318239927251526	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	4.0
16	13.0
17	15.0
18	31.0
19	54.0
20	86.0
21	164.0
22	263.0
23	432.0
24	771.0
25	1174.0
26	1847.0
27	2093.0
28	2063.0
29	2055.0
30	2367.0
31	2853.0
32	3839.0
33	5701.0
34	18207.0
35	76692.0
36	13688.0
37	20882.0
38	43520.0
39	288913.0
40	2726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.00737883239509	22.234568983621397	23.516230780680058	21.241821403303447
2	26.82273879259548	24.277561539543733	25.6986851038929	23.20101456396789
3	26.121963801107945	24.86069115131398	26.329321428789882	22.688023618788193
4	26.085263336031495	27.074748652787093	22.398089944684244	24.441898066497163
5	24.119341756769707	35.16985179128853	21.593330302146775	19.11747614979499
6	86.53847330143111	2.570663687132613	7.641852394807291	3.2490106166289805
7	86.26485094513893	2.1842893464666626	6.090238287964082	5.460621420430333
8	75.64557137526837	4.963941793062389	12.385999180356281	7.004487651312958
9	43.7624501230485	26.46755984724451	15.071045983643828	14.698944046063161
10	33.26611711118406	21.148846891776447	32.86547036743283	12.719565629606672
11	32.1477723837156	20.16323551300114	27.71945348929672	19.969538613986547
12	20.946382659438033	29.30491358059932	31.54343805879007	18.20526570117258
13	15.91209830831245	32.52170934455008	31.005164571002148	20.56102777613532
14	14.946060510911252	24.21639409774965	41.33532603265934	19.502219358679763
15	17.212518120854632	21.404934581421	42.19472858986619	19.18781870785818
16	16.895059097943346	23.52826037756623	30.889558106011332	28.687122418479095
17	18.612029189103225	23.475452486150672	34.61200472212651	23.300513602619596
18	21.839019526686336	28.725250123864072	29.900072789255734	19.535657560193858
19	23.614914253441178	25.612439010963246	27.507406357743903	23.265240377851676
20	19.058959297145314	31.747941205854946	28.41574286838602	20.77735662861372
21	18.93275047557686	25.41262536776924	28.3170593956249	27.337564761029
22	17.360135547051016	29.616663642276492	27.15487800153734	25.86832280913515
23	17.082231469833236	24.463102779652445	35.76460321699966	22.69006253351466
24	18.829173607472217	25.05173746118416	32.728659189286724	23.3904297420569
25	15.595250959819108	29.852973859074293	30.857547144805764	23.69422803630084
26	17.529365469347976	27.249891427790818	29.270863704667278	25.949879398193932
27	18.582668817042062	27.17302434260292	34.285166691473464	19.959140148881556
28	16.882825609584533	26.126041630560888	31.825216073988138	25.165916685866446
29	18.604892987560582	27.916208760401013	28.810680650903137	24.668217601135268
30	23.485443168310372	25.383672778653377	27.380585861757506	23.750298191278745
31	24.7860668723252	26.78420330426521	24.892498221046903	23.53723160236269
32	20.802027496804	27.700491582340554	26.781960498066088	24.715520422789357
33	16.379009780673943	26.568893909150038	31.627237454047957	25.424858856128058
34	18.680536723912596	27.687034745145855	30.592284338892096	23.040144192049457
35	21.644303170308508	29.65112130115382	27.92986948906836	20.77470603946931
36	20.700081760480533	28.24834796934288	28.552961829477407	22.498608440699186
37	20.12408835025293	29.75449427778582	29.813622804853434	20.30779456710782
38	20.16731334245408	29.185025394682917	26.025930917491237	24.621730345371766
39	22.984074037071547	25.364099197279273	26.69856888575349	24.953257879895688
40	16.823289299571623	22.064931278379145	30.862032757203995	30.249746664845233
41	20.45826647392126	26.22411342890406	25.79125183247461	27.526368264700064
42	19.770336645210488	24.229239260526406	29.39197523941956	26.60844885484354
43	21.59679645718177	26.98462862187715	28.63268339528236	22.785891525658723
44	17.148496198443492	28.45142387609923	29.13507198388442	25.265007941572858
45	20.77735662861372	27.93720958208365	28.563564186055046	22.721869603247583
46	22.31979561918782	23.17838261050408	29.691491812737915	24.810329957570186
47	20.425847729770396	21.77622095311108	32.22525114332143	25.572680173797092
48	19.122369545138515	20.914575589705112	29.857255579999876	30.105799285156497
49	20.41687650497393	23.89852729189307	31.49307686504627	24.191519338086724
50	19.69632404063965	24.59665169423619	29.92657868069984	25.78044558442432
51	18.711528227754933	21.577222875807664	32.34187706567548	27.369371830761924
52	20.19524647420671	20.57958190014619	34.84525656683461	24.379915058812497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	151.5
2	254.0
3	1131.5
4	2009.0
5	1386.0
6	763.0
7	745.5
8	728.0
9	748.5
10	769.0
11	758.5
12	748.0
13	759.0
14	750.5
15	731.0
16	696.5
17	662.0
18	681.5
19	701.0
20	809.5
21	918.0
22	1177.0
23	1436.0
24	1798.5
25	2161.0
26	2566.0
27	2971.0
28	3584.0
29	4197.0
30	5068.0
31	5939.0
32	6727.5
33	7516.0
34	8841.5
35	10167.0
36	11178.0
37	12189.0
38	13616.5
39	17083.0
40	19122.0
41	24133.0
42	29144.0
43	38592.5
44	48041.0
45	57689.5
46	67338.0
47	66786.5
48	66235.0
49	58063.5
50	49892.0
51	42863.0
52	35834.0
53	31449.5
54	27065.0
55	23516.5
56	19968.0
57	17525.5
58	15083.0
59	13608.5
60	12134.0
61	11370.5
62	10607.0
63	9085.5
64	6180.0
65	4796.0
66	3811.5
67	2827.0
68	2000.5
69	1174.0
70	1015.0
71	856.0
72	687.5
73	519.0
74	470.5
75	422.0
76	600.0
77	778.0
78	508.5
79	239.0
80	142.5
81	46.0
82	34.5
83	23.0
84	24.0
85	25.0
86	13.5
87	2.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	490457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.826437523139717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62050775986681	22.794256155949444
2	10.077797722215797	6.414807991538816
3	3.513975856003651	3.355119991167648
4	1.846689091074695	2.350941399270098
5	1.2236699353053802	1.9472527372470552
6	0.9986075141992404	1.9069271796479992
7	0.8460378421126294	1.8848459366946684
8	0.7685689367176487	1.9568648997336135
9	0.7445328062527135	2.1326244157916885
>10	8.134404992000139	46.31821487114964
>50	0.1705142835045602	3.6165428788240015
>100	0.04761530935599039	2.756231631149051
>500	0.0038607007585938157	0.7304761296016542
>1k	0.003217250632161513	1.834893782234601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2492	0.5080975498361732	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	2319	0.47282432506825267	TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1705	0.3476349608630318	TruSeq Adapter, Index 15 (95% over 22bp)
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG	1334	0.27199122451101726	TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1110	0.22631953463810284	No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	665	0.13558782931021476	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	634	0.12926719365815964	TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	627	0.12783995334963105	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	575	0.11723759677199021	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	548	0.11173252701052283	TruSeq Adapter, Index 14 (95% over 21bp)
CTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTGAAA	518	0.10561578283111465	TruSeq Adapter, Index 14 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4822033328100119	0.0
2	0.0	0.0	0.0	1.57689664945143	0.0
3	0.0	0.0	0.0	2.4685140593364965	0.0
4	0.0	0.0	0.0	3.0510319966888026	0.0
5	0.0	0.0	0.0	5.828849420030706	0.0
6	0.0	0.0	0.0	6.373443543470682	0.0
7	0.0	0.0	0.0	9.049315230489116	0.0
8	0.0	0.0	0.0	11.898086886312154	0.0
9	0.0	0.0	0.0	14.721983782472266	0.0
10	0.0	0.0	0.0	15.93350691294038	0.0
11	0.0	0.0	0.0	17.11933971785498	0.0
12	0.0	0.0	0.0	18.07661018193236	0.0
13	0.0	0.0	0.0	18.482150321027124	0.0
14	0.0	0.0	0.0	18.760258289717548	0.0
15	0.0	0.0	0.0	19.737714009586977	0.0
16	0.0	0.0	0.0	20.76410368289167	0.0
17	0.0	0.0	0.0	22.028434704775343	0.0
18	0.0	0.0	0.0	22.679052393991725	0.0
19	0.0	0.0	0.0	23.4269263156607	0.0
20	0.0	0.0	0.0	24.252890671353452	0.0
21	0.0	0.0	0.0	25.04908687203975	0.0
22	0.0	0.0	0.0	25.87178896417015	0.0
23	0.0	0.0	0.0	26.4948813045792	0.0
24	2.0389147264693948E-4	0.0	0.0	27.007668358286253	0.0
25	2.0389147264693948E-4	0.0	0.0	27.516581474013012	0.0
26	2.0389147264693948E-4	0.0	0.0	27.992056388225674	0.0
27	2.0389147264693948E-4	0.0	0.0	28.46936632569216	0.0
28	2.0389147264693948E-4	0.0	0.0	28.965026495696872	0.0
29	2.0389147264693948E-4	0.0	0.0	29.549379456303	0.0
30	2.0389147264693948E-4	0.0	0.0	30.124557300640014	0.0
31	2.0389147264693948E-4	0.0	0.0	30.61328516057473	0.0
32	2.0389147264693948E-4	0.0	0.0	31.09018731509592	0.0
33	4.0778294529387896E-4	0.0	0.0	31.570351733179464	0.0
34	4.0778294529387896E-4	0.0	0.0	32.14226731395413	0.0
35	4.0778294529387896E-4	0.0	0.0	32.60673208864386	0.0
36	4.0778294529387896E-4	0.0	0.0	33.0834303516924	0.0
37	4.0778294529387896E-4	0.0	0.0	33.54850680080007	0.0
38	4.0778294529387896E-4	0.0	0.0	34.00665093983774	0.0
39	4.0778294529387896E-4	0.0	0.0	34.51862242765421	0.0
40	4.0778294529387896E-4	0.0	0.0	35.02325382245538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGG	35	1.01796104E-7	46.000004	1
ATGGGCG	25	3.4142708E-5	46.0	4
CGGCGCT	25	3.4142708E-5	46.0	16
GGTCGGC	20	6.307898E-4	46.0	2
CATGCGG	65	0.0	46.0	1
ATTACGG	50	1.6370905E-11	46.0	1
GGATCGA	25	3.4142708E-5	46.0	7
CCAGCGG	60	0.0	46.0	1
AGGGTCG	20	6.307898E-4	46.0	5
CACGAGG	50	1.6370905E-11	46.0	1
TACGGGT	20	6.307898E-4	46.0	3
TGCGGGT	40	5.6006684E-9	46.0	3
ACGGGTA	30	1.8592054E-6	46.0	4
TCGGCGT	20	6.307898E-4	46.0	4
AGTGCGG	45	3.092282E-10	46.0	1
CTAACGC	20	6.307898E-4	46.0	44
ACGGCGC	25	3.4142708E-5	46.0	15
CTACGTC	20	6.307898E-4	46.0	27
GGAGTAA	25	3.4142708E-5	46.0	7
CTCGCGG	55	1.8189894E-12	46.0	1
>>END_MODULE