SRR1544938_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544938_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1693186
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	6541	0.3863131398440573	Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	6070	0.3584957588829579	Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	5957	0.35182194986256676	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3287	0.1941310641595194	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2612	0.15426539080762539	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2388	0.14103589328047836	Illumina PCR Primer Index 2 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2186	0.12910572140331897	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1873	0.11061986101940366	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1816	0.10725342638079928	Illumina PCR Primer Index 2 (95% over 24bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG	1790	0.10571785970354114	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	20	6.3123053E-4	46.0	14
CACGTTA	20	6.3123053E-4	46.0	3
CCAACCG	20	6.3123053E-4	46.0	21
CGAATAT	165	0.0	44.60606	14
ATAGCGG	185	0.0	43.51351	1
CGAGGGT	415	0.0	43.228916	3
TTAATCG	150	0.0	42.93333	19
CGTTTTT	2430	0.0	42.687244	1
CGGTCTA	200	0.0	42.55	30
GCCGATA	65	0.0	42.461536	8
ACGGGTA	240	0.0	42.166668	4
CATGCGG	260	0.0	41.57692	1
TATACGG	155	0.0	41.54839	1
TCGCAGG	295	0.0	41.322033	1
GCTACGA	195	0.0	41.28205	10
CATACGA	380	0.0	41.157894	17
ACGGGCG	205	0.0	40.390244	4
GCGTAGG	245	0.0	40.367348	1
GAATCGT	40	2.9116563E-7	40.25	8
CACGAGG	240	0.0	40.25	1