##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544938_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1693186
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.856810179153385	34.0	33.0	34.0	31.0	34.0
2	33.18716608807302	34.0	33.0	34.0	31.0	34.0
3	33.25503400098985	34.0	34.0	34.0	31.0	34.0
4	36.54849260506524	37.0	37.0	37.0	35.0	37.0
5	36.546060503689496	37.0	37.0	37.0	35.0	37.0
6	36.64671689938376	37.0	37.0	37.0	35.0	37.0
7	36.48499042633237	37.0	37.0	37.0	35.0	37.0
8	36.504404713953456	37.0	37.0	37.0	35.0	37.0
9	38.23502202356977	39.0	39.0	39.0	37.0	39.0
10	38.1353844173056	39.0	39.0	39.0	35.0	39.0
11	37.775777734991905	39.0	37.0	39.0	35.0	39.0
12	37.75007293941717	39.0	37.0	39.0	35.0	39.0
13	37.73868316888989	39.0	37.0	39.0	35.0	39.0
14	39.152839676208046	41.0	38.0	41.0	35.0	41.0
15	39.19222696147972	41.0	38.0	41.0	35.0	41.0
16	39.180416091321334	41.0	38.0	41.0	35.0	41.0
17	39.21447082600494	41.0	38.0	41.0	35.0	41.0
18	39.20090586621907	41.0	38.0	41.0	35.0	41.0
19	39.159688894191184	41.0	38.0	41.0	35.0	41.0
20	39.10011599434439	41.0	38.0	41.0	35.0	41.0
21	39.041841829545014	41.0	38.0	41.0	35.0	41.0
22	38.99928478028994	41.0	38.0	41.0	35.0	41.0
23	38.97673911785238	41.0	38.0	41.0	35.0	41.0
24	38.91516820951744	41.0	38.0	41.0	35.0	41.0
25	38.87814038150564	41.0	37.0	41.0	35.0	41.0
26	38.83255235987068	41.0	37.0	41.0	35.0	41.0
27	38.76599499405263	41.0	37.0	41.0	35.0	41.0
28	38.804595006100925	41.0	37.0	41.0	35.0	41.0
29	38.809909838611944	41.0	37.0	41.0	35.0	41.0
30	38.757217458684394	41.0	37.0	41.0	35.0	41.0
31	38.70784780880541	41.0	37.0	41.0	35.0	41.0
32	38.66638042128862	41.0	37.0	41.0	35.0	41.0
33	38.55672796727589	41.0	37.0	41.0	35.0	41.0
34	38.48844249834336	41.0	36.0	41.0	35.0	41.0
35	38.41539559150619	40.0	36.0	41.0	35.0	41.0
36	38.349864692951634	40.0	36.0	41.0	35.0	41.0
37	38.301796140530335	40.0	36.0	41.0	35.0	41.0
38	38.227697370519245	40.0	36.0	41.0	35.0	41.0
39	38.162426337094686	40.0	36.0	41.0	35.0	41.0
40	38.078265471129576	40.0	35.0	41.0	35.0	41.0
41	38.05928232338326	40.0	35.0	41.0	35.0	41.0
42	37.99392269957347	40.0	35.0	41.0	35.0	41.0
43	37.8980153391299	40.0	35.0	41.0	35.0	41.0
44	37.81966718364078	40.0	35.0	41.0	35.0	41.0
45	37.75320549543878	40.0	35.0	41.0	35.0	41.0
46	37.767194507868595	40.0	35.0	41.0	35.0	41.0
47	37.719978194953185	40.0	35.0	41.0	35.0	41.0
48	37.70286017011716	40.0	35.0	41.0	35.0	41.0
49	37.66027063772084	40.0	35.0	41.0	35.0	41.0
50	37.58158052334475	40.0	35.0	41.0	34.0	41.0
51	37.50852830108447	39.0	35.0	41.0	34.0	41.0
52	37.30182862367159	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	5.0
14	20.0
15	21.0
16	36.0
17	76.0
18	133.0
19	262.0
20	394.0
21	596.0
22	1033.0
23	1649.0
24	2777.0
25	4529.0
26	6597.0
27	7925.0
28	8506.0
29	9422.0
30	11378.0
31	13873.0
32	18092.0
33	24823.0
34	66962.0
35	215450.0
36	64613.0
37	100731.0
38	183909.0
39	942508.0
40	6858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.09802585185561	21.985298720872958	24.724395311560574	19.192280115710854
2	27.785252181390586	23.72037094566102	26.218915110330464	22.27546176261793
3	27.822164841901596	23.908832225166048	25.382917175077047	22.88608575785531
4	25.81494295369794	27.243197144318465	22.68870637957082	24.253153522412777
5	23.898319499452512	35.736593617003685	20.868646445222204	19.496440438321603
6	86.77316018440975	2.7859904346008055	7.534080721196608	2.9067686597928404
7	86.86310895554298	2.5915050089003806	5.687798032820966	4.857588002735671
8	75.51379470418489	5.22647836681853	12.171019604461648	7.088707324534931
9	42.60512430412252	21.150127629214982	20.72973672118716	15.515011345475335
10	32.94635084391201	23.177607185507085	28.041278394694974	15.834763575885932
11	31.62127492195187	19.018702020923868	28.264644285979212	21.09537877114505
12	21.788805246440734	25.897154831188068	31.781505398698073	20.532534523673124
13	17.17773475566181	28.368413157207772	31.858697154358705	22.59515493277171
14	16.67979773043245	25.225403470144446	37.075548699315966	21.019250100107133
15	18.78063012569204	19.851156340768235	39.889238394364234	21.478975139175496
16	18.545865604841996	23.667866377350155	29.274929039101433	28.511338978706412
17	20.416599239540133	23.398669726775438	31.78983289490936	24.39489813877507
18	22.423053344405165	28.384182245778078	28.02793077665419	21.16483363316257
19	24.412793396590807	24.261599139137697	26.780932514206945	24.54467495006455
20	21.616172115762826	29.493333868812993	27.228491140370874	21.6620028750533
21	20.5619465315683	23.759764136958374	27.804151463572225	27.874137867901105
22	19.595366368491117	27.81448700851531	25.59582940090457	26.99431722208901
23	19.511559864066914	23.12539791848031	34.4157109732776	22.94733124417518
24	21.86759162903544	22.44124390350499	30.643886731877064	25.047277735582508
25	19.099732693277645	26.667064339062573	29.382418706509505	24.85078426115028
26	19.27874433169185	26.318490703324976	27.66754508955307	26.735219875430104
27	21.491850275161735	25.52507521323706	31.163853232899395	21.819221278701807
28	19.796939025009657	24.286463507257917	28.693362690218322	27.223234777514104
29	21.411941747687496	24.86602180740923	27.84478492026275	25.87725152464053
30	24.615842559529785	24.295676907321464	24.569244016900683	26.519236516248068
31	23.75769702796976	25.34972531074554	26.578178652552054	24.31439900873265
32	24.17365841673626	25.38155878917024	23.74836550739257	26.696417286700928
33	18.036529950046834	23.34829132771001	29.97597428752659	28.639204434716564
34	20.571455232915937	24.686478626683662	28.586877047176152	26.15518909322425
35	24.04602920175338	25.20331493409466	27.723002670704815	23.027653193447144
36	21.667377358423707	26.70061056493498	26.889426205980914	24.7425858706604
37	20.379332217488212	28.982226406313305	28.56785964448088	22.070581731717603
38	20.291155254059507	27.678766538348416	25.55112078649363	26.47895742109845
39	23.63437921173456	24.17761545394304	27.415180612171376	24.77282472215102
40	17.301052571897007	22.96203724812277	30.60833245727286	29.128577722707373
41	20.13665362222461	25.496312868166875	25.59104552010234	28.775987989506174
42	20.906799371126386	21.816150145347294	29.007149834690342	28.269900648835982
43	22.804759784217445	24.01029774637872	28.682318422193426	24.502624047210407
44	19.619285772502256	25.948655375133033	29.060363126083015	25.371695726281697
45	21.450212794105315	25.298815369368754	27.746567713175047	25.504404123350888
46	22.54046513495859	23.458143405390782	27.86303453961939	26.13835692003123
47	20.348030281374875	21.378690823099173	31.850901200458782	26.42237769506717
48	20.354999391679353	20.863567263135888	28.375972870080428	30.405460475104327
49	20.462784360371515	23.10000200804873	31.38332114723368	25.05389248434608
50	19.8460180984251	23.174890413693475	30.220011268697	26.759080219184423
51	19.472993516365005	21.726496675498144	30.511296455321506	28.289213352815345
52	19.840997976595602	20.97920724598479	33.91257664544828	25.267218131971326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	496.0
2	812.0
3	3729.5
4	6647.0
5	4448.5
6	2250.0
7	2318.5
8	2387.0
9	2380.5
10	2374.0
11	2296.0
12	2218.0
13	2045.5
14	1869.0
15	1865.0
16	1735.0
17	1605.0
18	1674.0
19	1743.0
20	2334.5
21	2926.0
22	3253.0
23	3580.0
24	4019.5
25	4459.0
26	6504.0
27	8549.0
28	11376.0
29	14203.0
30	15258.5
31	16314.0
32	19107.5
33	21901.0
34	26042.5
35	30184.0
36	33431.0
37	36678.0
38	40774.0
39	50233.0
40	55596.0
41	65475.0
42	75354.0
43	94508.0
44	113662.0
45	132374.5
46	151087.0
47	170272.5
48	189458.0
49	197003.0
50	204548.0
51	184993.0
52	165438.0
53	140658.0
54	115878.0
55	100841.5
56	85805.0
57	77395.5
58	68986.0
59	66678.5
60	64371.0
61	63095.0
62	61819.0
63	54705.5
64	39834.5
65	32077.0
66	25671.0
67	19265.0
68	15700.5
69	12136.0
70	10046.0
71	7956.0
72	6797.5
73	5639.0
74	4858.5
75	4078.0
76	3303.0
77	2528.0
78	1789.5
79	1051.0
80	870.5
81	690.0
82	508.5
83	327.0
84	249.0
85	171.0
86	108.5
87	46.0
88	26.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1693186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.37145806862993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60939162579933	16.96482369191008
2	12.609782374649047	6.146375647966161
3	4.719483514098311	3.450620837083116
4	2.262305995435027	2.205427828246194
5	1.2766460749359232	1.5556863141890918
6	0.8381343187343031	1.225593324294767
7	0.5815460416514061	0.9921187478359471
8	0.4244075069666524	0.8274743808039627
9	0.37826674436837665	0.8297020889217943
>10	6.061880181300121	38.81339917448631
>50	1.0712639065778076	16.329592183930412
>100	0.15349165137318752	6.2365922221626375
>500	0.00755276340776303	1.2725303099424279
>1k	0.005116388114936246	2.0475322046448947
>5k	7.309125878480351E-4	1.1025310435822406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	6541	0.3863131398440573	Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	6070	0.3584957588829579	Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	5957	0.35182194986256676	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3287	0.1941310641595194	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2612	0.15426539080762539	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2388	0.14103589328047836	Illumina PCR Primer Index 2 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2186	0.12910572140331897	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1873	0.11061986101940366	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1816	0.10725342638079928	Illumina PCR Primer Index 2 (95% over 24bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG	1790	0.10571785970354114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36475614610562573	0.0
2	0.0	0.0	0.0	1.361693281187064	0.0
3	5.9060256817620745E-5	0.0	0.0	2.1799140791383818	0.0
4	5.9060256817620745E-5	0.0	0.0	2.690785300610801	0.0
5	5.9060256817620745E-5	0.0	0.0	5.41960540661215	0.0
6	5.9060256817620745E-5	0.0	0.0	5.940575932000383	0.0
7	5.9060256817620745E-5	0.0	0.0	8.192307283428992	0.0
8	5.9060256817620745E-5	0.0	0.0	10.544441071447554	0.0
9	1.1812051363524149E-4	0.0	0.0	12.644682864138966	0.0
10	1.1812051363524149E-4	0.0	0.0	13.76558747828059	0.0
11	1.1812051363524149E-4	0.0	0.0	14.851410299872548	0.0
12	1.1812051363524149E-4	0.0	0.0	15.730167861061927	0.0
13	1.1812051363524149E-4	0.0	0.0	16.093329380233477	0.0
14	1.1812051363524149E-4	0.0	0.0	16.357151547437788	0.0
15	1.1812051363524149E-4	0.0	0.0	17.29815861931294	0.0
16	1.1812051363524149E-4	0.0	0.0	18.334429885434915	0.0
17	1.7718077045286224E-4	0.0	0.0	19.579065737609454	0.0
18	1.7718077045286224E-4	0.0	0.0	20.205636002187592	0.0
19	2.3624102727048298E-4	0.0	0.0	20.956469047110005	0.0
20	3.543615409057245E-4	0.0	0.0	21.81939845947226	0.0
21	4.134217977233452E-4	0.0	0.0	22.64447024721442	0.0
22	4.134217977233452E-4	0.0	0.0	23.501021151840376	0.0
23	4.134217977233452E-4	0.0	0.0	24.16869735516358	0.0
24	4.134217977233452E-4	0.0	0.0	24.750854306614865	0.0
25	4.134217977233452E-4	0.0	0.0	25.27152953071901	0.0
26	4.134217977233452E-4	0.0	0.0	25.79279535739133	0.0
27	4.7248205454096596E-4	0.0	0.0	26.322388680274937	0.0
28	4.7248205454096596E-4	0.0	0.0	26.837984722292767	0.0
29	4.7248205454096596E-4	0.0	0.0	27.46443686635727	0.0
30	4.7248205454096596E-4	0.0	0.0	28.046003215240383	0.0
31	4.7248205454096596E-4	0.0	0.0	28.59195622926247	0.0
32	4.7248205454096596E-4	0.0	0.0	29.113517357218875	0.0
33	5.315423113585867E-4	0.0	0.0	29.61824631198226	0.0
34	5.315423113585867E-4	0.0	0.0	30.2240273661606	0.0
35	5.315423113585867E-4	0.0	0.0	30.718302655467266	0.0
36	5.315423113585867E-4	0.0	0.0	31.239155060341865	0.0
37	5.315423113585867E-4	0.0	0.0	31.751030306180184	0.0
38	5.315423113585867E-4	0.0	0.0	32.250266657059534	0.0
39	5.315423113585867E-4	0.0	0.0	32.801653214708836	0.0
40	5.906025681762074E-4	0.0	0.0	33.31967072725619	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	20	6.3123053E-4	46.0	14
CACGTTA	20	6.3123053E-4	46.0	3
CCAACCG	20	6.3123053E-4	46.0	21
CGAATAT	165	0.0	44.60606	14
ATAGCGG	185	0.0	43.51351	1
CGAGGGT	415	0.0	43.228916	3
TTAATCG	150	0.0	42.93333	19
CGTTTTT	2430	0.0	42.687244	1
CGGTCTA	200	0.0	42.55	30
GCCGATA	65	0.0	42.461536	8
ACGGGTA	240	0.0	42.166668	4
CATGCGG	260	0.0	41.57692	1
TATACGG	155	0.0	41.54839	1
TCGCAGG	295	0.0	41.322033	1
GCTACGA	195	0.0	41.28205	10
CATACGA	380	0.0	41.157894	17
ACGGGCG	205	0.0	40.390244	4
GCGTAGG	245	0.0	40.367348	1
GAATCGT	40	2.9116563E-7	40.25	8
CACGAGG	240	0.0	40.25	1
>>END_MODULE