FastQCFastQC Report
Thu 26 May 2016
SRR1544937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544937_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences882742
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT42960.48666541299722904Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT36450.41291793072041433Illumina PCR Primer Index 2 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA30350.34381506714306104No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT30150.34154939948478713Illumina PCR Primer Index 2 (96% over 25bp)
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTTG21550.24412569017901042Illumina PCR Primer Index 2 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.23982092162829002No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13150.1489676485315075No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT11500.13027589035074802Illumina PCR Primer Index 2 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC11360.1286899229899563Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC10080.11418964997700347Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC9740.11033801495793788Illumina PCR Primer Index 2 (95% over 24bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG9180.10399414551477103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAT206.3106534E-446.00000434
ATAGCGG1100.046.01
ATTGCGG1400.044.3571431
CATGCGG2100.043.8095251
CGGTCTA850.043.29411730
CGTTTTT15200.043.1251
TAGGGCG1550.043.0322574
CGATTAA750.042.93333423
ACGGGTA1450.042.8275834
CTAGCGG2150.042.7906951
GCGATAG601.8189894E-1242.1666688
TCGACAA601.8189894E-1242.16666818
CGAGGGA4300.041.720933
ATCGCGG1550.041.5483861
ACCGGGT1000.041.3999983
CCAACGG508.712959E-1041.3999981
ACATACG1950.041.2820516
TCACGAC950.041.15789424
CGAGGGT2350.041.1063843
GGCGATA1400.041.071437