SRR1544937_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544937_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	882742
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	4296	0.48666541299722904	Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	3645	0.41291793072041433	Illumina PCR Primer Index 2 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3035	0.34381506714306104	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3015	0.34154939948478713	Illumina PCR Primer Index 2 (96% over 25bp)
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTTG	2155	0.24412569017901042	Illumina PCR Primer Index 2 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2117	0.23982092162829002	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1315	0.1489676485315075	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1150	0.13027589035074802	Illumina PCR Primer Index 2 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1136	0.1286899229899563	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1008	0.11418964997700347	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	974	0.11033801495793788	Illumina PCR Primer Index 2 (95% over 24bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG	918	0.10399414551477103	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAT	20	6.3106534E-4	46.000004	34
ATAGCGG	110	0.0	46.0	1
ATTGCGG	140	0.0	44.357143	1
CATGCGG	210	0.0	43.809525	1
CGGTCTA	85	0.0	43.294117	30
CGTTTTT	1520	0.0	43.125	1
TAGGGCG	155	0.0	43.032257	4
CGATTAA	75	0.0	42.933334	23
ACGGGTA	145	0.0	42.827583	4
CTAGCGG	215	0.0	42.790695	1
GCGATAG	60	1.8189894E-12	42.166668	8
TCGACAA	60	1.8189894E-12	42.166668	18
CGAGGGA	430	0.0	41.72093	3
ATCGCGG	155	0.0	41.548386	1
ACCGGGT	100	0.0	41.399998	3
CCAACGG	50	8.712959E-10	41.399998	1
ACATACG	195	0.0	41.28205	16
TCACGAC	95	0.0	41.157894	24
CGAGGGT	235	0.0	41.106384	3
GGCGATA	140	0.0	41.07143	7