FastQCFastQC Report
Thu 26 May 2016
SRR1544936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544936_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1608968
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT60890.37844133630998256Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT50510.31392793393032054Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT49680.30876934780554993Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32640.2028629531476077No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26820.16669069863415556No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT25230.15680858786501659No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT20590.1279702268783469Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC19850.12337100551409351No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC17640.10963549306139089No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC17100.1062793044982871No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16630.10335817741558564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG206.3122105E-446.012
ACCGTAG206.3122105E-446.08
CTAACGG1300.045.9999961
CGGTCTA900.043.44444730
CGTTTTT25700.042.3307381
TAGTAGG2500.042.321
CGCGCAT601.8189894E-1242.16666836
CGAGGGA4500.041.911113
CGAATAT1650.041.8181814
TCGATGG1600.041.68751
CATGCGG2650.041.6603741
TACGAAA5700.041.56140519
CATACGA5700.041.56140517
ACATACG5700.041.56140516
TAGCGCA508.731149E-1041.423
TACACGG1000.041.41
TACGGGA2400.041.2083323
AAATCGG950.041.1578941
TTTACGG950.041.1578941
CACGACG950.041.15789425
Produced by FastQC (version 0.11.5)