##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544936_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1608968
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88080061256657	34.0	33.0	34.0	31.0	34.0
2	33.201480079156326	34.0	33.0	34.0	31.0	34.0
3	33.26933351067268	34.0	34.0	34.0	31.0	34.0
4	36.55570713649992	37.0	37.0	37.0	35.0	37.0
5	36.55064799299924	37.0	37.0	37.0	35.0	37.0
6	36.641585786665736	37.0	37.0	37.0	35.0	37.0
7	36.4557772435499	37.0	37.0	37.0	35.0	37.0
8	36.48441734080479	37.0	37.0	37.0	35.0	37.0
9	38.27463131647118	39.0	39.0	39.0	37.0	39.0
10	38.11759711815275	39.0	39.0	39.0	35.0	39.0
11	37.59552582773554	39.0	37.0	39.0	35.0	39.0
12	37.533060943412174	39.0	37.0	39.0	35.0	39.0
13	37.51357329667215	39.0	37.0	39.0	35.0	39.0
14	38.85789462562338	41.0	37.0	41.0	35.0	41.0
15	38.91214244161475	41.0	37.0	41.0	35.0	41.0
16	38.90081033308307	41.0	37.0	41.0	35.0	41.0
17	38.932548689594825	41.0	37.0	41.0	35.0	41.0
18	38.91831720705446	41.0	37.0	41.0	35.0	41.0
19	38.86971151694751	41.0	37.0	41.0	35.0	41.0
20	38.80627458097364	41.0	36.0	41.0	35.0	41.0
21	38.75720834721387	41.0	35.0	41.0	35.0	41.0
22	38.72482920729312	41.0	35.0	41.0	35.0	41.0
23	38.7117201833722	41.0	35.0	41.0	35.0	41.0
24	38.6390549718826	41.0	35.0	41.0	35.0	41.0
25	38.60415247537552	41.0	35.0	41.0	35.0	41.0
26	38.54814763251973	40.0	35.0	41.0	35.0	41.0
27	38.48967226197165	40.0	36.0	41.0	35.0	41.0
28	38.530646352195944	40.0	36.0	41.0	35.0	41.0
29	38.528684224919324	40.0	36.0	41.0	35.0	41.0
30	38.477254364288164	40.0	35.0	41.0	35.0	41.0
31	38.425687770048874	40.0	35.0	41.0	35.0	41.0
32	38.35876288403498	40.0	35.0	41.0	35.0	41.0
33	38.262237657927315	40.0	35.0	41.0	35.0	41.0
34	38.16696292281761	40.0	35.0	41.0	35.0	41.0
35	38.10697105225213	40.0	35.0	41.0	35.0	41.0
36	38.055425589570454	40.0	35.0	41.0	35.0	41.0
37	37.996705341560556	40.0	35.0	41.0	35.0	41.0
38	37.90546114030857	40.0	35.0	41.0	35.0	41.0
39	37.82613327300481	40.0	35.0	41.0	35.0	41.0
40	37.732196041189134	40.0	35.0	41.0	35.0	41.0
41	37.686561199476934	40.0	35.0	41.0	35.0	41.0
42	37.61605016383172	40.0	35.0	41.0	35.0	41.0
43	37.5229190387876	40.0	35.0	41.0	34.0	41.0
44	37.45374550643643	39.0	35.0	41.0	34.0	41.0
45	37.38937008069769	39.0	35.0	41.0	34.0	41.0
46	37.399197498023575	39.0	35.0	41.0	34.0	41.0
47	37.339894889146336	39.0	35.0	41.0	34.0	41.0
48	37.31651779277151	39.0	35.0	41.0	34.0	41.0
49	37.25754955971778	39.0	35.0	41.0	34.0	41.0
50	37.18251388467639	39.0	35.0	41.0	34.0	41.0
51	37.11153857627995	39.0	35.0	41.0	34.0	41.0
52	36.88487216650673	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	6.0
14	21.0
15	28.0
16	39.0
17	70.0
18	147.0
19	268.0
20	431.0
21	749.0
22	1133.0
23	1837.0
24	3069.0
25	4980.0
26	6859.0
27	8291.0
28	8711.0
29	9780.0
30	11354.0
31	13975.0
32	18016.0
33	25016.0
34	76078.0
35	260800.0
36	62322.0
37	101842.0
38	170236.0
39	815086.0
40	7817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.97637491858136	21.92343166551479	23.99538089011093	20.10481252579293
2	27.381340088802265	24.27698997121136	25.62617777357909	22.715492166407287
3	27.346286563809848	24.03037226346329	25.47900268992298	23.14433848280388
4	26.595867661755857	26.30108243296324	23.00841284599818	24.094637059282718
5	24.37059034113792	35.240228519150165	20.754235012753515	19.634946126958397
6	86.39158765121493	3.19633454487597	7.487470229364412	2.924607574544677
7	85.93719701075472	3.143008437706654	5.913604248188902	5.006190303349724
8	74.79135694432705	5.738647381427101	11.900174521805281	7.56982115244057
9	48.709607649126646	19.55775379000701	16.32226371189483	15.410374848971514
10	37.927603283595445	22.238167570765857	24.584951347696162	15.249277797942531
11	37.32709413736009	17.29760939931683	26.07516122135431	19.300135241968768
12	24.579171245170816	26.006670114010973	28.622570492390153	20.791588148428062
13	16.621772465331816	28.933577299237772	30.828145743109868	23.616504492320544
14	16.131582480198485	23.893203593856434	39.36454920172434	20.610664724220744
15	19.712200615549843	18.47793119564839	41.222075268122175	20.58779292067959
16	18.993851959765514	21.96600553895416	29.114749330005317	29.925393171275005
17	20.727136897688457	22.703994112996654	31.006272343514603	25.56259664580029
18	24.040565132432715	28.011557718985085	27.12533748340551	20.822539665176684
19	27.47133566360549	23.06161464988738	25.466820968471716	24.00022871803541
20	23.76914892030171	28.109508703715676	26.027739519990455	22.093602855992163
21	20.752246160271678	24.428702124591663	25.938365461587797	28.88068625354886
22	20.728069172289317	27.786817388537248	24.733183009233247	26.751930429940185
23	22.088817179707736	21.403408893153873	33.4186882523456	23.089085674792788
24	21.99366301877974	21.348653298263233	30.397061967671203	26.260621715285826
25	18.961595258575684	27.209552955683396	28.338910407167823	25.4899413785731
26	19.58659215099368	26.892828197950486	27.13111758593086	26.389462065124974
27	21.184945878351837	26.278894297462724	30.78451529178952	21.751644532395918
28	19.66204424202346	25.62344310141656	27.87718587318082	26.83732678337916
29	21.944003858373815	25.35215119256567	27.017069326425386	25.68677562263513
30	24.59166372482237	23.916137549037643	23.650501439431984	27.841697286708
31	26.213386468842142	23.881581237165687	25.287948548386296	24.61708374560588
32	24.40856499321304	25.77297994739485	23.06062022364646	26.757834835745648
33	19.042330239010347	23.040234485707607	29.942795630491098	27.974639644790944
34	19.652162131254318	23.08405139194813	29.164408490411247	28.099377986386305
35	25.064637705659777	24.698626697361288	26.727877745237944	23.50885785174099
36	20.991778581053197	26.72023309351087	28.42878167869094	23.859206646744994
37	22.504425196772093	25.715241073781453	27.54597978331452	24.234353946131932
38	21.662084019073095	25.064699857299832	26.475169176764236	26.798046946862836
39	26.26975800637427	20.682263413566957	29.263788962863153	23.78418961719562
40	17.811479159312054	20.072120763122697	32.50089498361683	29.615505093948418
41	20.984444687526416	24.132363104797612	25.254697420955548	29.628494786720434
42	21.450333381397268	21.171272517539194	28.794295473868964	28.584098627194578
43	22.050221011232043	25.001305184441208	28.979880271080592	23.968593533246153
44	19.664281701065526	25.906854580078658	27.68917716200695	26.739686556848863
45	21.398933975069735	24.276244151530673	26.97921897763038	27.34560289576921
46	22.305477796948107	22.046181154628307	28.327225898837018	27.321115149586568
47	21.53187633315268	19.618724548903398	32.145760512328394	26.703638605615527
48	20.708367102391097	19.375773787918714	29.85286220732793	30.06299690236226
49	20.18710129722903	22.603805669224002	31.54096290292908	25.66813013061789
50	19.668570164229493	22.451099089602778	29.75764589475987	28.122684851407858
51	19.99617145897246	20.489655481028834	30.913542096548845	28.600630963449863
52	21.59663834209257	18.449154986301778	33.852382396666684	26.101824274938966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	507.0
2	832.0
3	3834.5
4	6837.0
5	4454.5
6	2072.0
7	2246.5
8	2421.0
9	2402.0
10	2383.0
11	2292.5
12	2202.0
13	2089.0
14	1967.5
15	1959.0
16	2025.5
17	2092.0
18	2099.5
19	2107.0
20	2407.5
21	2708.0
22	2678.5
23	2649.0
24	3127.0
25	3605.0
26	5013.5
27	6422.0
28	8855.0
29	11288.0
30	12632.0
31	13976.0
32	14822.0
33	15668.0
34	18811.5
35	21955.0
36	24538.0
37	27121.0
38	29768.5
39	38244.0
40	44072.0
41	55072.5
42	66073.0
43	85093.0
44	104113.0
45	119230.0
46	134347.0
47	151336.5
48	168326.0
49	173644.5
50	178963.0
51	162372.5
52	145782.0
53	132546.0
54	119310.0
55	105802.5
56	92295.0
57	84615.0
58	76935.0
59	74994.0
60	73053.0
61	75156.5
62	77260.0
63	69308.0
64	49656.0
65	37956.0
66	30320.0
67	22684.0
68	18467.5
69	14251.0
70	12036.5
71	9822.0
72	8564.0
73	7306.0
74	6200.0
75	5094.0
76	4500.0
77	3906.0
78	2838.0
79	1770.0
80	1364.5
81	959.0
82	665.5
83	372.0
84	211.5
85	51.0
86	30.5
87	10.0
88	18.0
89	14.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1608968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.194577109603163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89676946990576	15.980314320722822
2	12.6792219510801	5.881783824682008
3	4.909527405847429	3.416232359599145
4	2.496332804440798	2.31605534895336
5	1.4345871072345835	1.6637320639597541
6	0.8626000544685082	1.2004586086870621
7	0.624439867516861	1.0138533060201151
8	0.470058399914175	0.8722244642260809
9	0.39510848459018766	0.8247936791237006
>10	5.567489173635979	33.7567768109357
>50	1.4505113283177349	21.534985188257217
>100	0.19855639017575233	7.31539718586237
>500	0.009685677516330055	1.4927713599843113
>1k	0.004573792160489193	2.035438461962639
>5k	5.380931953516697E-4	0.6951830170237544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	6089	0.37844133630998256	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5051	0.31392793393032054	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	4968	0.30876934780554993	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3264	0.2028629531476077	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2682	0.16669069863415556	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2523	0.15680858786501659	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	2059	0.1279702268783469	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1985	0.12337100551409351	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1764	0.10963549306139089	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1710	0.1062793044982871	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1663	0.10335817741558564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31523311837152757	0.0
2	0.0	0.0	0.0	1.239303702746108	0.0
3	0.0	0.0	0.0	2.002277236091706	0.0
4	6.215164005747784E-5	0.0	0.0	2.473262364447273	0.0
5	6.215164005747784E-5	0.0	0.0	4.941055384569488	0.0
6	6.215164005747784E-5	0.0	0.0	5.444670123955231	0.0
7	6.215164005747784E-5	0.0	0.0	7.631786337577876	0.0
8	6.215164005747784E-5	0.0	0.0	9.961291958572202	0.0
9	6.215164005747784E-5	0.0	0.0	11.967360444707415	0.0
10	1.8645492017243352E-4	0.0	0.0	13.064771953202301	0.0
11	1.8645492017243352E-4	0.0	0.0	14.132164219549425	0.0
12	1.8645492017243352E-4	0.0	0.0	15.00931031568061	0.0
13	1.8645492017243352E-4	0.0	0.0	15.381847246185133	0.0
14	1.8645492017243352E-4	0.0	0.0	15.652890548475794	0.0
15	1.8645492017243352E-4	0.0	0.0	16.522019083039563	0.0
16	1.8645492017243352E-4	0.0	0.0	17.463305671710064	0.0
17	1.8645492017243352E-4	0.0	0.0	18.66469687402111	0.0
18	1.8645492017243352E-4	0.0	0.0	19.30175118461026	0.0
19	2.4860656022991136E-4	0.0	0.0	20.074171767244593	0.0
20	3.1075820028738917E-4	0.0	0.0	20.91607788346319	0.0
21	3.1075820028738917E-4	0.0	0.0	21.709878630277295	0.0
22	3.1075820028738917E-4	0.0	0.0	22.534071529079508	0.0
23	3.1075820028738917E-4	0.0	0.0	23.13290258103331	0.0
24	3.1075820028738917E-4	0.0	0.0	23.666723017486987	0.0
25	3.1075820028738917E-4	0.0	0.0	24.15890185510215	0.0
26	3.1075820028738917E-4	0.0	0.0	24.636661512223984	0.0
27	3.1075820028738917E-4	0.0	0.0	25.12200366943283	0.0
28	3.1075820028738917E-4	0.0	0.0	25.610950621765006	0.0
29	3.1075820028738917E-4	0.0	0.0	26.200210321149953	0.0
30	3.1075820028738917E-4	0.0	0.0	26.772067561318806	0.0
31	3.1075820028738917E-4	0.0	0.0	27.321985272547373	0.0
32	3.1075820028738917E-4	0.0	0.0	27.83535781942214	0.0
33	3.1075820028738917E-4	0.0	0.0	28.31659796838719	0.0
34	3.1075820028738917E-4	0.0	0.0	28.91953102858478	0.0
35	3.1075820028738917E-4	0.0	0.0	29.442288473108228	0.0
36	3.7290984034486703E-4	0.0	0.0	29.98655038509156	0.0
37	4.3506148040234484E-4	0.0	0.0	30.519003485463973	0.0
38	4.3506148040234484E-4	0.0	0.0	31.040269290626043	0.0
39	4.3506148040234484E-4	0.0	0.0	31.607403006150527	0.0
40	4.3506148040234484E-4	0.0	0.0	32.190198934969494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	6.3122105E-4	46.0	12
ACCGTAG	20	6.3122105E-4	46.0	8
CTAACGG	130	0.0	45.999996	1
CGGTCTA	90	0.0	43.444447	30
CGTTTTT	2570	0.0	42.330738	1
TAGTAGG	250	0.0	42.32	1
CGCGCAT	60	1.8189894E-12	42.166668	36
CGAGGGA	450	0.0	41.91111	3
CGAATAT	165	0.0	41.81818	14
TCGATGG	160	0.0	41.6875	1
CATGCGG	265	0.0	41.660374	1
TACGAAA	570	0.0	41.561405	19
CATACGA	570	0.0	41.561405	17
ACATACG	570	0.0	41.561405	16
TAGCGCA	50	8.731149E-10	41.4	23
TACACGG	100	0.0	41.4	1
TACGGGA	240	0.0	41.208332	3
AAATCGG	95	0.0	41.157894	1
TTTACGG	95	0.0	41.157894	1
CACGACG	95	0.0	41.157894	25
>>END_MODULE