FastQCFastQC Report
Thu 26 May 2016
SRR1544935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544935_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences905995
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT44600.4922764474417629Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT37050.40894265420890846Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT30420.33576344240310374Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA30390.3354323147478739No Hit
TGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTG21840.24106093300735654Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.2238422949354025No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15330.1692062318224714No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT10930.12064084238875492Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC10860.1198682111932185No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC10680.1178814452618392No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC10120.11170039569754801No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT9980.11015513330647521No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC206.3107425E-446.013
AATCTCG206.3107425E-446.017
CGTACAT405.6097633E-946.034
CGGTCTA650.046.030
ATTGCGC206.3107425E-446.013
TTCGACA206.3107425E-446.014
CGACGGT700.045.99999627
TCGGCGT5050.044.6336634
ACGGGAT2750.044.3272744
CGAATAT1000.043.69999714
GTCGGCG5250.043.371433
CGTTTTT14500.043.1448251
TCGATGG800.043.1251
CACGACG750.042.9333325
ATGATCG700.042.71428326
GTCCCCC5200.042.461549
ATAAGCG601.8189894E-1242.16666832
ATTGGGC1200.042.1666683
TACGGGA1650.041.818183
TAGTAGG1650.041.818181
Produced by FastQC (version 0.11.5)