##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544935_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	905995
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.874794011004475	34.0	33.0	34.0	31.0	34.0
2	33.24927621013361	34.0	33.0	34.0	31.0	34.0
3	33.38176369626764	34.0	34.0	34.0	31.0	34.0
4	36.60449450604032	37.0	37.0	37.0	35.0	37.0
5	36.61114354935734	37.0	37.0	37.0	35.0	37.0
6	36.397898443148144	37.0	37.0	37.0	35.0	37.0
7	36.20385542966573	37.0	37.0	37.0	35.0	37.0
8	36.34375907151806	37.0	37.0	37.0	35.0	37.0
9	38.22773414864321	39.0	39.0	39.0	37.0	39.0
10	38.154616747333044	39.0	39.0	39.0	35.0	39.0
11	37.682501559059375	39.0	37.0	39.0	35.0	39.0
12	37.66636018962577	39.0	37.0	39.0	35.0	39.0
13	37.654929662967234	39.0	37.0	39.0	35.0	39.0
14	38.92685390095972	41.0	38.0	41.0	35.0	41.0
15	38.95487723442182	41.0	38.0	41.0	35.0	41.0
16	38.966606879728914	41.0	38.0	41.0	35.0	41.0
17	39.0223246265156	41.0	38.0	41.0	35.0	41.0
18	39.02902444273975	41.0	38.0	41.0	35.0	41.0
19	39.018297010469155	41.0	37.0	41.0	35.0	41.0
20	38.9634865534578	41.0	37.0	41.0	35.0	41.0
21	38.915273263097475	41.0	37.0	41.0	35.0	41.0
22	38.88799055182424	41.0	36.0	41.0	35.0	41.0
23	38.85401906191535	41.0	36.0	41.0	35.0	41.0
24	38.802236215431655	41.0	36.0	41.0	35.0	41.0
25	38.76313997317866	41.0	36.0	41.0	35.0	41.0
26	38.68694198091601	41.0	35.0	41.0	35.0	41.0
27	38.753932416845565	41.0	36.0	41.0	35.0	41.0
28	38.764633358903744	41.0	36.0	41.0	35.0	41.0
29	38.76785633474798	41.0	36.0	41.0	35.0	41.0
30	38.728228080728925	41.0	36.0	41.0	35.0	41.0
31	38.675914326238	41.0	36.0	41.0	35.0	41.0
32	38.60652321480803	41.0	36.0	41.0	35.0	41.0
33	38.5288472894442	41.0	36.0	41.0	35.0	41.0
34	38.42598358710589	41.0	35.0	41.0	35.0	41.0
35	38.37654512442121	40.0	35.0	41.0	35.0	41.0
36	38.31057676918747	40.0	35.0	41.0	35.0	41.0
37	38.276809474665974	40.0	35.0	41.0	35.0	41.0
38	38.180651107346065	40.0	35.0	41.0	35.0	41.0
39	38.132898084426515	40.0	35.0	41.0	35.0	41.0
40	38.06487563397149	40.0	35.0	41.0	35.0	41.0
41	38.02129813078439	40.0	35.0	41.0	35.0	41.0
42	37.96783536332982	40.0	35.0	41.0	35.0	41.0
43	37.905933255702294	40.0	35.0	41.0	35.0	41.0
44	37.77824601681025	40.0	35.0	41.0	35.0	41.0
45	37.7683828277198	40.0	35.0	41.0	35.0	41.0
46	37.698052417507824	40.0	35.0	41.0	35.0	41.0
47	37.60911704810733	39.0	35.0	41.0	35.0	41.0
48	37.53364312165078	39.0	35.0	41.0	35.0	41.0
49	37.48125100028146	39.0	35.0	41.0	35.0	41.0
50	37.409401817890824	39.0	35.0	41.0	35.0	41.0
51	37.33161662040077	39.0	35.0	41.0	35.0	41.0
52	36.86804452563204	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	7.0
15	13.0
16	15.0
17	34.0
18	58.0
19	142.0
20	205.0
21	344.0
22	479.0
23	857.0
24	1455.0
25	2459.0
26	3489.0
27	4022.0
28	4077.0
29	4154.0
30	4809.0
31	5955.0
32	7881.0
33	11513.0
34	40525.0
35	141385.0
36	33120.0
37	56233.0
38	99422.0
39	479794.0
40	3541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25779943597923	21.893277556719408	23.086882377938068	21.762040629363298
2	27.64838658049989	24.37640384328832	25.611620373180866	22.363589203030923
3	27.777195238384316	23.72198522066899	25.559412579539618	22.941406961407072
4	26.654672487155008	25.961953432414088	23.379047345735906	24.004326734695002
5	24.20278257606278	34.70604142406967	20.8524329604468	20.238743039420747
6	85.277622944939	3.115359356287838	8.138345134355045	3.4686725644181258
7	84.93336055938498	3.0404141303208077	6.523325183913818	5.502900126380388
8	73.25349477646124	5.7570957897118635	12.750953371707348	8.238456062119548
9	48.25832372143334	19.00606515488496	16.607155668629517	16.12845545505218
10	37.074487166043966	21.8621515571278	25.58568204018786	15.477679236640379
11	36.49512414527674	17.242368887245515	26.227628187793535	20.034878779684213
12	24.2055419731897	25.15422270542332	29.312192672144988	21.328042649241993
13	16.60848017925044	28.04485675969514	31.077765329830736	24.26889773122368
14	16.147219355515208	23.633463760837532	38.77239940617774	21.446917477469523
15	19.645583033018944	18.247451696753295	40.93896765434688	21.167997615880882
16	18.896241149233717	22.39515670616284	29.254797211905142	29.4538049326983
17	20.434880987201918	22.93290801825617	31.194211888586583	25.437999105955335
18	23.54317628684485	28.27189995529777	27.122666239879912	21.06225751797747
19	26.900921086760967	22.920545919127587	25.784579385095945	24.393953609015504
20	23.46326414604937	28.479958498667212	26.023653552171922	22.033123803111497
21	20.530025000137968	24.242407518805294	25.94131314190476	29.286254339151984
22	20.60441834667962	27.83138979795694	24.708414505598817	26.855777349764626
23	21.886213500074504	21.50729308660644	33.2593446983703	23.347148714948755
24	22.06965822107186	21.610273787382933	29.801930474229994	26.518137517315214
25	18.984321105524867	26.904673866853567	28.337352855148207	25.773652172473362
26	19.516222495709137	26.045176849761866	27.157986523104434	27.280614131424567
27	21.722194934850634	25.798707498385753	30.491890131843995	21.98720743491962
28	20.03432690025883	25.19517215878675	27.822780478920965	26.947720462033452
29	22.196590488909983	24.90664959519644	26.631493551288916	26.26526636460466
30	24.485565593629104	23.526951031738584	23.26160740401437	28.72587597061794
31	26.233698861472742	23.391850948404795	25.119012798083872	25.255437392038587
32	23.749248064282916	25.692305145171883	23.089862526835137	27.468584263710067
33	18.652751946754673	22.706968581504313	30.197959149884934	28.44232032185608
34	19.941390405024308	22.491735605604887	29.20325167357436	28.363622315796444
35	24.953669722239084	24.295056815986843	27.088780843161388	23.662492618612685
36	20.924839541057068	26.441978156612343	28.391657790605905	24.24152451172468
37	22.925954337496343	25.074641692283066	27.854679109708115	24.144724860512476
38	21.66071556686295	24.57872284063378	26.296502740081344	27.464058852421925
39	26.706880280796252	20.277153847427414	29.038018973614644	23.97794689816169
40	17.895131871588696	19.493374687498275	32.85558971075999	29.755903730153037
41	21.090955248097394	23.959845253009124	25.375747106772113	29.573452392121368
42	21.389742768999827	20.70850280630688	28.9466277407712	28.955126683922096
43	22.566791207456998	24.57872284063378	28.863514699308496	23.990971252600733
44	19.8172175343131	24.980601438197784	28.209206452574243	26.992974574914875
45	21.350559329797626	23.75101407844414	27.071451829204356	27.82697476255388
46	22.543281143935673	21.284775302291955	28.635146993084952	27.53679656068742
47	21.559832007902912	19.204300244482585	32.2680588744971	26.9678088731174
48	20.660820423953773	18.79237744137661	29.552260222186653	30.99454191248296
49	20.216778238290498	21.746036126027185	31.63648806008863	26.400697575593686
50	19.952759121187203	21.738530565841973	30.045640428479185	28.26306988449164
51	20.14525466476084	19.901213582856418	30.893879105293077	29.059652647089663
52	21.537204951462204	18.35595119178362	33.89720693822814	26.20963691852604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	101.0
1	319.0
2	537.0
3	2338.0
4	4139.0
5	2765.0
6	1391.0
7	1439.5
8	1488.0
9	1478.0
10	1468.0
11	1393.5
12	1319.0
13	1264.0
14	1202.5
15	1196.0
16	1199.5
17	1203.0
18	1169.5
19	1136.0
20	1300.5
21	1465.0
22	1410.5
23	1356.0
24	1608.0
25	1860.0
26	2536.5
27	3213.0
28	4252.5
29	5292.0
30	6062.0
31	6832.0
32	7256.0
33	7680.0
34	9453.5
35	11227.0
36	12543.5
37	13860.0
38	15568.0
39	19927.5
40	22579.0
41	28462.0
42	34345.0
43	44385.0
44	54425.0
45	63699.0
46	72973.0
47	85485.0
48	97997.0
49	101305.5
50	104614.0
51	95716.5
52	86819.0
53	78446.5
54	70074.0
55	61585.5
56	53097.0
57	48161.5
58	43226.0
59	42180.5
60	41135.0
61	42180.5
62	43226.0
63	40075.0
64	29213.5
65	21503.0
66	17075.5
67	12648.0
68	10502.0
69	8356.0
70	7014.5
71	5673.0
72	4922.0
73	4171.0
74	3567.0
75	2963.0
76	2562.0
77	2161.0
78	1615.5
79	1070.0
80	786.5
81	503.0
82	360.0
83	217.0
84	123.5
85	30.0
86	16.0
87	2.0
88	8.5
89	7.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	905995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.368916621719475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1146957534304	20.17446874408015
2	11.300218532237652	6.411499146965184
3	3.9821121578897047	3.389046233565254
4	1.9615697728152588	2.2259043733072517
5	1.1812367288533314	1.6755203135676406
6	0.7863018276681295	1.3383918593173683
7	0.6309160611411676	1.2528883593672218
8	0.5362489626049327	1.2170241686898322
9	0.48106770859358133	1.2282632743043655
>10	7.622948767309197	47.09694485434216
>50	0.30240299490018724	5.6887153433393935
>100	0.09013560007993547	4.486016488169609
>500	0.005852961044151645	1.1309685253819486
>1k	0.004292171432377873	2.684348315602648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	4460	0.4922764474417629	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	3705	0.40894265420890846	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	3042	0.33576344240310374	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3039	0.3354323147478739	No Hit
TGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTG	2184	0.24106093300735654	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.2238422949354025	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1533	0.1692062318224714	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	1093	0.12064084238875492	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1086	0.1198682111932185	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1068	0.1178814452618392	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1012	0.11170039569754801	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	998	0.11015513330647521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4162274626239659	0.0
2	0.0	0.0	0.0	1.4726350586923769	0.0
3	0.0	0.0	0.0	2.2804761615682207	0.0
4	0.0	0.0	0.0	2.7991324455432975	0.0
5	0.0	0.0	0.0	5.549809877537956	0.0
6	0.0	0.0	0.0	6.082373523032688	0.0
7	0.0	0.0	0.0	8.44828061965022	0.0
8	0.0	0.0	0.0	10.980634550963305	0.0
9	0.0	0.0	0.0	13.339698342706086	0.0
10	1.1037588507662846E-4	0.0	0.0	14.560566007538673	0.0
11	1.1037588507662846E-4	0.0	0.0	15.689821687757659	0.0
12	1.1037588507662846E-4	0.0	0.0	16.667089774226127	0.0
13	1.1037588507662846E-4	0.0	0.0	17.074707917814116	0.0
14	1.1037588507662846E-4	0.0	0.0	17.36687288561195	0.0
15	1.1037588507662846E-4	0.0	0.0	18.267098604296933	0.0
16	1.1037588507662846E-4	0.0	0.0	19.251761875065537	0.0
17	1.1037588507662846E-4	0.0	0.0	20.38885424312496	0.0
18	1.1037588507662846E-4	0.0	0.0	21.064023532138698	0.0
19	1.1037588507662846E-4	0.0	0.0	21.83985562834232	0.0
20	1.1037588507662846E-4	0.0	0.0	22.694496106490654	0.0
21	1.1037588507662846E-4	0.0	0.0	23.496818415112667	0.0
22	1.1037588507662846E-4	0.0	0.0	24.321105524864926	0.0
23	1.1037588507662846E-4	0.0	0.0	24.919011694325025	0.0
24	1.1037588507662846E-4	0.0	0.0	25.4579771411542	0.0
25	1.1037588507662846E-4	0.0	0.0	25.954006368688567	0.0
26	1.1037588507662846E-4	0.0	0.0	26.442640411922802	0.0
27	1.1037588507662846E-4	0.0	0.0	26.9423120436647	0.0
28	1.1037588507662846E-4	0.0	0.0	27.42785556211679	0.0
29	1.1037588507662846E-4	0.0	0.0	28.01351000833338	0.0
30	1.1037588507662846E-4	0.0	0.0	28.57565439102865	0.0
31	1.1037588507662846E-4	0.0	0.0	29.11870374560566	0.0
32	1.1037588507662846E-4	0.0	0.0	29.639457171397193	0.0
33	1.1037588507662846E-4	0.0	0.0	30.107561300007173	0.0
34	1.1037588507662846E-4	0.0	0.0	30.689904469671465	0.0
35	1.1037588507662846E-4	0.0	0.0	31.20315233527779	0.0
36	1.1037588507662846E-4	0.0	0.0	31.742117782106966	0.0
37	1.1037588507662846E-4	0.0	0.0	32.25426188886252	0.0
38	1.1037588507662846E-4	0.0	0.0	32.75945231485825	0.0
39	1.1037588507662846E-4	0.0	0.0	33.328550378313345	0.0
40	1.1037588507662846E-4	0.0	0.0	33.90316723602228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAC	20	6.3107425E-4	46.0	13
AATCTCG	20	6.3107425E-4	46.0	17
CGTACAT	40	5.6097633E-9	46.0	34
CGGTCTA	65	0.0	46.0	30
ATTGCGC	20	6.3107425E-4	46.0	13
TTCGACA	20	6.3107425E-4	46.0	14
CGACGGT	70	0.0	45.999996	27
TCGGCGT	505	0.0	44.633663	4
ACGGGAT	275	0.0	44.327274	4
CGAATAT	100	0.0	43.699997	14
GTCGGCG	525	0.0	43.37143	3
CGTTTTT	1450	0.0	43.144825	1
TCGATGG	80	0.0	43.125	1
CACGACG	75	0.0	42.93333	25
ATGATCG	70	0.0	42.714283	26
GTCCCCC	520	0.0	42.46154	9
ATAAGCG	60	1.8189894E-12	42.166668	32
ATTGGGC	120	0.0	42.166668	3
TACGGGA	165	0.0	41.81818	3
TAGTAGG	165	0.0	41.81818	1
>>END_MODULE